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Gene screening and bioinformatics analysis method

A bioinformatics and molecular biology technology, applied in the field of genetic screening, can solve problems such as changing the characteristics of damage

Inactive Publication Date: 2020-04-17
JILIN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

These methods are all remedial measures for the damaged nerves, but they have no decisive effect and significance on fundamentally changing the characteristics of the damaged nerves.

Method used

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  • Gene screening and bioinformatics analysis method

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Embodiment

[0051] At the beginning of the experiment, that is, on the 0th day, 10 healthy adult male SD rats of SPF grade, weighing about 200 g, were anesthetized with pentobarbital sodium anesthetic (30 mg / kg) by intraperitoneal injection, and the left hip was tilted under aseptic conditions. Make an incision, bluntly separate the muscles, expose the sciatic nerve in the left gluteal muscle space of the rat, and transect the sciatic nerve 0.5 cm away from the lower edge of the piriformis muscle. Five of the rats were selected as the 0-day control group, and 0.5 cm of proximal and distal nerve tissues were excised to obtain the nerve fragments required for the experiment, and then the rats were sacrificed according to animal ethics. The above operations were repeated for the other 5 rats. After the sciatic nerve was exposed and transected, the proximal end was folded back and rinsed with saline to protect it from reconnection of the nerve stump, and then the wound was sutured. Then on th...

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Abstract

The invention belongs to the technical field of gene screening, and particularly relates to a gene screening and bioinformatics analysis method which comprises the following steps: step 1, preparing an experimental model; 2, carrying out RNA extraction and gene chip analysis; 3, carrying out a molecular biology experiment; and step 4, concluding a final result. The method is based on different changes of pathophysiology of the near end and the far end after nerve injury; an effective experimental model is designed; techniques using gene chips, starting from the overall angle of regeneration after peripheral nerve injury; according to the most important change characteristics in the damage and regeneration process, the method comprises the following steps: selecting genes with obvious expression difference by using a biotechnology software, performing axon guidance, axon regeneration, myelin sheath generation, extracellular matrix and signal transduction, performing comparative analysison the biological process of the genes at different time points by using the existing biotechnology software to obtain reliable data, and performing verification by using an experimental method commonly used in molecular biology.

Description

technical field [0001] The invention relates to the technical field of gene screening, in particular to a method for gene screening and bioinformatics analysis. Background technique [0002] With the process of social modernization, various types of traumatic incidents are increasing year by year. Among the many traumatic events, due to labor operations, the resulting limb injuries, especially the cases of peripheral nerve injuries, have gradually increased. [0003] Although there are more and more people suffering from peripheral nerve injury, due to the lack of specific and effective treatment methods at present, patients will suffer from neurological sequelae while suffering from pain. Limb dysfunction caused by peripheral nerve injury not only has a huge impact on the quality of life and work ability of patients, but also adds a heavy burden to society. [0004] Peripheral nerve injury refers to a clinical disease in which certain traumatic factors cause nerve or nerv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851G01N33/68G01N21/64
CPCC12Q1/6851G01N21/6428G01N33/6827C12Q2521/107C12Q2531/113C12Q2537/165
Inventor 姜南辛光大张春媚罗慧玲吴若然
Owner JILIN UNIV
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