Gene screening and bioinformatics analysis method

Abstract

The invention belongs to the technical field of gene screening, and particularly relates to a gene screening and bioinformatics analysis method which comprises the following steps: step 1, preparing an experimental model; 2, carrying out RNA extraction and gene chip analysis; 3, carrying out a molecular biology experiment; and step 4, concluding a final result. The method is based on different changes of pathophysiology of the near end and the far end after nerve injury; an effective experimental model is designed; techniques using gene chips, starting from the overall angle of regeneration after peripheral nerve injury; according to the most important change characteristics in the damage and regeneration process, the method comprises the following steps: selecting genes with obvious expression difference by using a biotechnology software, performing axon guidance, axon regeneration, myelin sheath generation, extracellular matrix and signal transduction, performing comparative analysison the biological process of the genes at different time points by using the existing biotechnology software to obtain reliable data, and performing verification by using an experimental method commonly used in molecular biology.

Description

technical field [0001] The invention relates to the technical field of gene screening, in particular to a method for gene screening and bioinformatics analysis. Background technique [0002] With the process of social modernization, various types of traumatic incidents are increasing year by year. Among the many traumatic events, due to labor operations, the resulting limb injuries, especially the cases of peripheral nerve injuries, have gradually increased. [0003] Although there are more and more people suffering from peripheral nerve injury, due to the lack of specific and effective treatment methods at present, patients will suffer from neurological sequelae while suffering from pain. Limb dysfunction caused by peripheral nerve injury not only has a huge impact on the quality of life and work ability of patients, but also adds a heavy burden to society. [0004] Peripheral nerve injury refers to a clinical disease in which certain traumatic factors cause nerve or nerv...

AI Technical Summary

Problems solved by technology

These methods are all remedial measures for the damaged nerves, but they have no decisive effect and significance on fundamentally changing the characteristics of the damaged nerves.

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