Polyiodocarboxylic acid modified Anderson polyacid derivatives and their application in anti-adv7 virus
A technology of iodocarboxylic acid and its derivatives, which is applied in antiviral agents, medical preparations containing active ingredients, compounds of Group 7/17 elements of the periodic table, etc., can solve the problems of unreported inhibitory activity and achieve The effect of susceptibility to resistance
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Embodiment 1
[0034] [Example 1] Anti-ADV7 Activity Research Experiment of Polyiodocarboxylic Acid Modified Anderson Polyacid Organic Derivatives (A4~A7)
[0035] In this example, the above-mentioned compounds (A4-A7) were subjected to anti-ADV7 activity research experiments, and the experimental conditions were as follows:
[0036] 1. Test content:
[0037] Analysis of compound anti-ADV7 activity: the present invention evaluates the anti-ADV7 activity of polyiodocarboxylic acid-modified Anderson polyacid organic derivatives (A4-A7) by combining cytopathic effect analysis and MTT assay cell viability detection method.
[0038] 2. Test method:
[0039] 2.1.1 Toxicity of compounds to host Hela cells
[0040] Plate HeLa cells in 96-well plates at 37°C, 5% CO 2 After the incubator was full of monolayers, the cell culture medium was discarded, and cell maintenance solutions containing different concentrations of test compounds were added to continue the culture. SPSS 11.5 software was used t...
Embodiment 2
[0052] [Example 2] Inhibitory effect test of compounds A4, A5 and A7 on the production of ADV7 progeny virus
[0053] In this example, an in-depth study on the anti-ADV7 activity of the iodocarboxylic acid-modified Anderson polyacid organic derivatives was carried out, and the inhibitory effect test of compounds A4, A5, and A7 on the production of ADV7 progeny virus was carried out. The test conditions are as follows:
[0054] 1. Test content
[0055] The inhibitory effects of compounds A4, A5, and A7 on the virus production of ADV7 progeny were detected after ADV7 infection of Hela cells.
[0056] 2. Test method
[0057] HeLa cells in logarithmic growth phase were plated in 24-well plates, 100 TCID after confluent monolayer 50 ADV7-infected cells, incubated at 37°C for 1.5 h, removed the virus solution, washed three times with PBS, and added cell maintenance solution containing 100 μg / mL A4, A5, and A7. Cells and supernatant culture medium were collected after 48h, and aft...
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