Immune targeting functional liposome as well as preparation method and application thereof

A liposome and functional technology, applied in the field of medicine, can solve problems such as inability to obtain teaching and prompting

Active Publication Date: 2020-04-28
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
View PDF10 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Then, on the basis of PEGylated phospholipids, modify mannose (Man) or galactose (Gal) to obtain PEGylated phospholipids modified by carbohydrate ligands, and then use PEGylated phospholipids modified by carbohydrate ligands as membrane materials ,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immune targeting functional liposome as well as preparation method and application thereof
  • Immune targeting functional liposome as well as preparation method and application thereof
  • Immune targeting functional liposome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0048] Mannose-modified phospholipids: Accurately weigh 4-aminophenyl α-D-mannopyranoside, add DMSO solvent, and dissolve with magnetic stirring to make a solution with a concentration of 1 mg / mL; add distearoylphosphatidylethanolamine- PEG 2000 - Aminosuccinimide, the amount added is about 5 times the mass of 4-aminophenyl α-D-mannopyranoside; filled with nitrogen protection, stirred at room temperature and protected from light for 48 hours; dialyzed the reacted solution in DMSO 24 hours to remove unreacted 4-aminophenyl α-D-mannopyranoside, then dialyzed in pure water for 24 hours to remove DMSO, freeze-dried to obtain mannose-modified phospholipids: distearoylphosphatidylethanolamine -PEG 2000 - Mannose;

[0049] Galactose-modified phospholipids: Accurately weigh 4-aminophenyl α-D-galactopyranoside, add DMSO, and dissolve with magnetic stirring to make a solution with a concentration of 1mg / mL; add distearoylphosphatidylethanolamine- PEG 2000 - Aminosuccinimide, the add...

Embodiment 1

[0050] Embodiment 1: the liposome of mannose modification

[0051] Soy Lecithin, Cholesterol, Distearoyl Phosphatidylethanolamine-PEG 2000 and distearoylphosphatidylethanolamine-PEG 2000 - Mannose, add chloroform to dissolve to prepare solutions with concentrations of 20mg / ml, 10mg / ml, 30mg / mL and 30mg / mL, store at -20°C for later use; measure according to the specified mass ratio and the ratio in Table 1 Put various lipid material solutions in a round bottom flask, 40°C, 100rpm under reduced pressure rotary evaporation to remove chloroform, continue rotary evaporation for 1h, add 4mL 55°C preheated PBS (10mM, pH7.4) buffer, 55°C, 100rpm conditions After hydration for 1 h, the mannose-modified liposome suspension was obtained. According to the liposome particle size measurement method in the summary of the invention, the particle size of the prepared liposomes was measured, and the results are shown in Table 1.

[0052] Table 1 The composition of mannose-modified liposome f...

Embodiment 2

[0054] Embodiment 2: the liposome of galactose modification

[0055] Soy Lecithin, Cholesterol, Distearoyl Phosphatidylethanolamine-PEG 2000 and distearoylphosphatidylethanolamine-PEG 2000 -Galactose, add chloroform to dissolve and make solutions with concentrations of 20mg / ml, 10mg / ml, 30mg / mL and 30mg / mL respectively, store at -20°C for later use; Phosphatidylethanolamine-PEG 2000 15% by weight, distearoylphosphatidylethanolamine-PEG 2000 - The weight percentage of galactose is 10%, and the weight percentage of cholesterol is 15%. Measure the above solution in a round bottom flask, remove chloroform by rotary evaporation at 40°C and 100rpm under reduced pressure, continue rotary evaporation for 1h, add 4mL of PBS preheated at 55°C (10 mM, pH 7.4) buffer solution, hydrated for 1 h at 55° C. and 100 rpm to obtain a galactose-modified liposome suspension. According to the liposome particle size measurement method in the summary of the invention, the particle size of the pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of medicines, and particularly relates to an immune targeting functional liposome as well as a preparation method and application thereof. The functional liposome disclosed by the invention is a blank liposome which does not entrap a drug, a membrane material contains carbohydrate ligand modified PEGylated phospholipid, the average particle size is 50-500nm, and the functional liposome has a targeting function on immunosuppressive cells in a tumor microenvironment and can promote tumor-associated macrophages to differentiate into M1 type macrophages; the polypeptide has a certain capacity of inhibiting tumor growth, can be used as an adjuvant of a tumor immune treatment, and can also be used as an entrapped delivery tool of an immunomodulator oran anti-tumor drug.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an immune-targeting functional liposome, a preparation method and application of the liposome. Background technique [0002] Immunotherapy is a therapeutic measure to fight diseases by regulating the body's own immune system. It has important clinical significance for the treatment of viral infections, autoimmune diseases and cancer, especially the immunotherapy of cancer, which has attracted widespread attention at home and abroad. . Different from chemotherapy, tumor immunotherapy acts on the cells of the immune system, especially the immune cells in the tumor microenvironment, to stimulate and enhance the immune function of the body, and improve the anti-tumor immunity of the tumor microenvironment, so as to control and kill tumor cells antitumor therapy. Its target is immune cells in the tumor microenvironment rather than tumor cells, which has the advantage of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K47/69A61K47/60A61K47/54A61K9/127A61P35/00
CPCA61K47/6911A61K47/60A61K47/549A61K9/1277A61P35/00
Inventor 刘玉玲叶军杨艳芳陈晓光孟盈盈高越刘东东冯遇季鸣金晶
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap