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Method and product for constructing calcium bisphosphonate crystals on surface of composite support frame

A technology of composite scaffold and calcium bisphosphonate, which can be applied in the fields of coating, medical science, and prosthesis, etc., can solve the problems of toxicity, etc., and achieve the effect of long acting time, good promotion of cell osteogenic differentiation, and good cytocompatibility

Inactive Publication Date: 2020-04-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of constructing calcium bisphosphonate crystals on composite scaffolds while taking into account the biological properties of materials such as cytotoxicity and osteogenic differentiation, the present invention provides a method and product for constructing calcium bisphosphonate crystals on the surface of composite scaffolds

Method used

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  • Method and product for constructing calcium bisphosphonate crystals on surface of composite support frame
  • Method and product for constructing calcium bisphosphonate crystals on surface of composite support frame
  • Method and product for constructing calcium bisphosphonate crystals on surface of composite support frame

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Step 1: Synthesis of modified gelatin (GelMA)

[0045] (1) Add 5g of gelatin into 100mL of PBS solution, stir at 50°C until dissolved;

[0046] (2) According to the feeding ratio (gelatin: methacrylic anhydride = 1g: 1mL), add 5mL methacrylic anhydride and react at 50°C for 3h;

[0047] (3) Transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 5000, and dialyze at 40°C for 7 days;

[0048] (4) Use a lyophilizer to freeze the pre-frozen dialysate to obtain dry modified gelatin GelMA. figure 1 Shown are the infrared spectra of gelatin (Gelatin) and modified gelatin (GelMA). Compared with Gelatin, GelMA has characteristic peaks of double bonds at 5.3 and 5.6ppm, indicating that the double bond has been successfully introduced into the gelatin molecular chain to obtain modification. gelatin.

[0049] Step 2: Preparation of hydroxyapatite-modified gelatin (HAp-GelMA) composite scaffold

[0050] (1) Dissolve 0.5g GelMA in 10mL deionized water...

Embodiment 2

[0059] Step 1: Synthesis of modified gelatin (GelMA)

[0060] (1) Add 10g of gelatin into 100mL of PBS solution, stir at 50°C until dissolved;

[0061] (2) According to the feeding ratio (gelatin: methacrylic anhydride = 1g: 2mL), add 20mL methacrylic anhydride and react at 55°C for 2h;

[0062] (3) Transfer the reaction solution to a dialysis bag with a molecular weight cut off of 7000, and dialyze at 40°C for 5 days;

[0063] (4) Use a lyophilizer to freeze the pre-frozen dialysate to obtain dry modified gelatin GelMA.

[0064] Step 2: Preparation of hydroxyapatite-modified gelatin (HAp-GelMA) composite scaffold

[0065] (1) Dissolve 1g of GelMA in 10mL of deionized water at 50°C to obtain a GelMA solution;

[0066] (2) Add 1g of hydroxyapatite and 0.1g of photoinitiator Irgacure 2959 in sequence, and stir until uniformly mixed;

[0067] (3) Transfer the pre-solution to the mold, and cross-link under ultraviolet light (365nm, 10mW) for 10min to obtain the HAp-GelMA compo...

Embodiment 3

[0074] Step 1: Synthesis of modified gelatin (GelMA)

[0075] (1) Add 15g of gelatin into 100mL of PBS solution, stir at 50°C until dissolved;

[0076] (2) According to the feeding ratio (gelatin: methacrylic anhydride = 1g: 3mL), add 45mL methacrylic anhydride and react at 60°C for 1h;

[0077] (3) Transfer the reaction solution to a dialysis bag with a molecular weight cut-off of 10 000, and dialyze at 40°C for 3 days;

[0078] (4) Use a lyophilizer to freeze the pre-frozen dialysate to obtain dry modified gelatin GelMA.

[0079] Step 2: Preparation of hydroxyapatite-modified gelatin (HAp-GelMA) composite scaffold

[0080] (1) Dissolve 1.5g of GelMA in 10mL of deionized water at 50°C to obtain a GelMA solution;

[0081] (2) Add 1.5g of hydroxyapatite and 0.15g of photoinitiator Irgacure 2959 in sequence, and stir until uniformly mixed;

[0082] (3) Transfer the pre-solution to the mold, and cross-link for 5 minutes under ultraviolet light (365nm, 15mW) and cure to obtain...

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Abstract

The invention discloses a method and a product for constructing calcium bisphosphonate crystals on the surface of a composite support frame. The method is based on a recrystallization technology. Firstly, a bioactive ceramic-biomedical high polymer composite support frame is immersed into a bisphosphonate water solution for incubation, and calcium bisphosphonate crystals are constructed on the surface of the bioactive ceramic-biomedical high polymer composite support frame. The invention effectively solves the problems that a bioactive ceramic-biomedical high polymer composite support frame lacks osteoinduction and cytotoxicity exists by directly using high-concentration bisphosphonate, and has great research significance on applications of bioactive ceramic-biomedical high polymer composite support frames in bone tissue engineering.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical bone repair materials, in particular to a method and product for constructing calcium bisphosphonate crystals on the surface of a composite bracket. Background technique [0002] Bone is a natural phosphorus-containing composite material. Bioactive ceramic materials (such as calcium phosphate, hydroxyapatite, β-tricalcium phosphate, and bioglass) have similar chemical compositions to the mineral phases of natural bone tissue, exhibiting good bioactivity, osteoconductivity, and mechanical strength , is an excellent choice for bone injury repair and regeneration and has been used in bone tissue engineering for decades. However, bioactive ceramic materials are fragile and their degradation properties cannot be regulated. Therefore, the combination of osteoconductive bioactive ceramic materials and biodegradable biomedical polymers has become a choice for bone tissue engineering. Although b...

Claims

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Application Information

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IPC IPC(8): A61L27/10A61L27/12A61L27/22A61L27/32A61L27/50
CPCA61L27/10A61L27/12A61L27/222A61L27/32A61L27/50A61L2420/02A61L2430/02
Inventor 王迎军刘磊施雪涛郑志雯朱光林张惠琳
Owner SOUTH CHINA UNIV OF TECH