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UIMC1 gene mutation site detection kit and application thereof

A detection kit and gene technology, applied in the field of UIMC1 gene mutation site detection kits, can solve the problems of different degrees of radiation damage, adverse reactions, poor treatment effects, etc., and achieve the effect of low cost and high efficiency

Pending Publication Date: 2020-04-28
CHINA INST FOR RADIATION PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even with the same radiation therapy plan, the degree of radiation injury in different patients is different. The reason is mainly related to the individual differences in susceptibility to radiation injury: under the same treatment conditions, radiation injury susceptible groups may cause more serious adverse effects. response, while those who are not susceptible to radiation injury may be at risk of suboptimal treatment

Method used

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  • UIMC1 gene mutation site detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Sample collection, irradiation and chromosome aberration analysis

[0022] Collect peripheral blood from healthy adult males aged 20-30, give 0, 2Gy 60 Co gamma ray irradiation. Chromosomal aberration analysis was performed on samples irradiated with 2Gy γ-rays, and the population was divided into susceptible group, general group and non-susceptible group. Chromosomes were harvested after blood samples were cultured for 52 hours after irradiation, and slides were prepared. 200 metaphases were analyzed per sample.

[0023] 2) Genomic DNA extraction from peripheral blood

[0024] Genomic DNA was extracted from the 0Gy irradiated samples of the susceptible group and the non-susceptible group. Genomic DNA extraction kit from Beijing Tiange Biochemical Technology Co., Ltd. was used to extract genomic DNA from whole blood according to the product instructions. See the instructions for specific steps. The A260 / 280 of the sampled nucleic acid quantitative detection is b...

Embodiment 2

[0032] 1) Using Beijing Tiange Biochemical Technology Co., Ltd. Blood Genomic DNA Extraction Kit (non-centrifugal column type; catalog number: DP319), perform human whole blood genome (source is the same as step 1) in Example 1) DNA extraction according to the product instructions.

[0033] 2) Using the amplification primer pair of SEQ ID NO.3 and SEQ ID NO.4, the specific reaction system (5 μl reaction system includes 0.95 μl H 2 O, 0.625μl PCR Buffer (10×), 0.325μl MgCl 2 (25mM), 1μl dNTP (2.5mM), 1μl primer, 0.1μl HotstarTaq (5U / μL)) for PCR reaction, the reaction program is: 94°C, 15min; [94°C, 20sec, 56°C, 30sec] 45 cycles ; 72°C, 4min. The reaction product was stored at 4°C.

[0034] 3) Use SAP reaction solution (2μl SAP reaction solution includes 1.53μl H 2 0, 0.17 μl SAP Buffer (10×), 0.3 μl SAP enzyme (1 U / μL)) to treat the reaction product of step 2), the treatment program is: 37° C., 40 min; 85° C., 5 min. The processed product was stored at 4°C.

[0035] 4) us...

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Abstract

The invention belongs to the technical field of biological detection, and relates to a UIMC1 gene mutation site detection kit and an application thereof. The detection kit comprises an amplification primer and an extension primer, wherein a forward primer of the amplification primer has a sequence as shown in SEQ ID NO. 3; a reverse primer of the amplification primer has a sequence as shown in SEQID NO. 4; the extension primer has a sequence as shown in SEQ ID NO. 5; the UIMC1 gene has a sequence as shown in SEQ ID NO. 1; and the mutated UIMC1 gene has a sequence as shown in SEQ ID NO. 2. TheUIMC1 gene mutation site detection kit provided by the invention can be used for detecting radiation damage with high efficiency and low cost.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a UIMC1 gene mutation site detection kit and application thereof. Background technique [0002] At present, there are about 200,000 radiation workers in our country. Radiation workers have to accept occupational exposure for a long time in their daily work, and the damage of cumulative exposure is obvious to susceptible people. Therefore, an effective method is needed to judge whether the personnel meet the job requirements to reduce occupational injuries. [0003] In addition, radiation therapy is one of the main treatments for malignant tumors, and more than 50% of cancer patients require radiation therapy. However, even with the same radiation therapy plan, the degree of radiation injury in different patients is different. The reason is mainly related to the individual differences in susceptibility to radiation injury: under the same treatment conditions, radiation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6883C12Q1/6858C12Q2600/156C12Q2600/172C12Q2533/101C12Q2531/113C12Q2565/627
Inventor 原雅艺董娟聪党旭红张忠新张睿凤刘红艳任越左雅慧
Owner CHINA INST FOR RADIATION PROTECTION