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Cell surface modification method

A cell surface and cell technology, applied in the field of cell surface modification, can solve the problems of biomass loss, restricted development, quantitative evaluation of biosorption performance, etc., and achieve the effect of improving stability and reducing dissolution

Inactive Publication Date: 2020-05-01
GUANGDONG UNIV OF PETROCHEMICAL TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The substantial loss of biomass during pretreatment may lead to some confusion in the quantitative assessment of biosorption performance
In addition, the poor mechanical stability of microorganisms may limit their further development in practical applications

Method used

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  • Cell surface modification method
  • Cell surface modification method
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Experimental program
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Embodiment 1

[0049] 1.1 Experiment

[0050] Media and Collection Techniques

[0051] Bacillus (Gram-positive bacteria), Escherichia coli (Gram-negative bacteria), Pseudomonas aeruginosa (Gram-negative bacteria), and Mucormyces ruckeri (fungi) were purchased from Guangdong Microbial Culture Collection Center (GMCCC, China). The growth medium of Bacillus contains maltose 10.0g / L, casein peptone 10.0g / L, beef extract 5.0g / L, NaCl 2.0g / L, maintain pH 7.2 and 32℃ in a rotary shaker, stirring speed It is 200r / min. The growth medium of Mucor ruckeri contains 50.0g / L glucose, 20.0g / L casein peptone, 2.0g / L yeast extract, 4.0g / L KH 2 PO 4 , 2.0g / L MgSO 4 , maintain pH 4.5 and 28° C. in a rotary vibrator with a stirring speed of 300 r / min. The growth medium of Escherichia coli and Pseudomonas aeruginosa contained 10.0 g / L casein peptone, 5.0 g / L yeast extract and 10.0 g / L NaCl, maintained at pH 7.0 and 37 °C in a rotary shaker with a stirring speed of 120r / min. Cells were centrifuged at 4°C, ...

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Abstract

The invention discloses a cell surface modification method. The method comprises the following steps of obtaining a cell suspension; centrifuging the suspension to form agglomerated cells; resuspending the obtained agglomerated cells by using a ClO2 solution; culturing for 0.1 to 120 minutes at room temperature; and washing with ddH2O to obtain the modified cell. The cells obtained by the modification method have high metal ion adsorption capacity, high mechanical stability and very strong NaOH cracking resistance.

Description

technical field [0001] The present application relates to the technical field of cell surface modification, in particular to a method for cell surface modification. Background technique [0002] Dyes and heavy metals are common pollutants in industrial wastewater, many of which are toxic and carcinogenic. Therefore, effective and thorough approaches to remediate wastewater contaminated with dyes and metals are urgently needed. Among the many technologies used to treat industrial dye and metal polluted wastewater, biosorption has become a favorable biological treatment method due to its environmental protection, good performance, low cost and containing a large amount of effective biomass. [0003] Many native microorganisms have been reported to be able to accumulate dyes or metals. However, further studies have shown that the adsorption capacity of cells can be improved by pretreatment of cell biomass with surface group modification. (Vijayaraghavan, K.; Yun, Y.S. Bacter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/38C12R1/07C12R1/19C12R1/385C12R1/785
CPCC12N1/14C12N1/20C12N1/38
Inventor 韦明肯李长秀向音波孙巍汤保贵韩寒冰
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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