Culture medium for selectively isolating bacteria of genus clostridium

Abstract

To provide a culture medium in which plural kinds of bacteria of genus Clostridium can be selectively detected on the same culture medium and identified for every species. A culture medium for detecting bacteria of genus Clostridium contains (a) cycloserine, (b) polymyxin B, (c) an aminoglycoside-based antibiotic, (d) sodium taurocholate and (e) a phosphatase substrate capable of releasing a colored chromogen compound.

Description

technical field [0001] The present invention relates to a culture medium for selecting and isolating bacteria of the genus Clostridium to detect said bacteria. Background technique [0002] Clostridium bacteria are Gram-positive obligate anaerobic spore-forming bacteria. Among the bacteria, especially Clostridium perfringens (hereinafter, also referred to as "Cp") or Clostridium difficile (hereinafter, also referred to as "Cd") is called pathogenic bacteria. Furthermore, Clostridium sporogenes (hereinafter, also referred to as "Cs") is a non-toxin producer, but is used as an indicator of food contamination. [0003] Cp is an inherent bacterium in the large intestine of humans or animals, and is also widely distributed in soil, sewage, rivers, oceans, etc., and a large number of foods such as meat, fish and shellfish, and vegetables are contaminated with Cp. In addition, Cp produces enterotoxin as a toxin when spores are formed and the spores are not completely killed by h...

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Problems solved by technology

If the analyte is applied to CCFA agar and then anaerobic culture is performed, Cd forms rough colonies, but the growth of most intestinal bacteria etc. is inhibited by cycloserine and cefoxitin (Non-Patent Document No. 5)

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