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Encapsulation method and application of zif-hemin based on melamine sponge

A technology of melamine sponge and hemin, which is applied in the field of nanomaterials, can solve problems such as easy agglomeration and difficult separation, and achieve the effects of simple synthesis method, excellent catalytic and cycle performance

Active Publication Date: 2021-06-11
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the MOFs material encapsulating enzymes is a nanomaterial, making it difficult to separate from aqueous solutions and easily causing agglomeration

Method used

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  • Encapsulation method and application of zif-hemin based on melamine sponge
  • Encapsulation method and application of zif-hemin based on melamine sponge
  • Encapsulation method and application of zif-hemin based on melamine sponge

Examples

Experimental program
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Effect test

Embodiment 1

[0027] The encapsulation method based on melamine sponge loaded ZIF-hemin provided by the present embodiment comprises the following steps:

[0028] 1) 1.0g Zn(NO 3 ) 2 ·6H 2 O. Dissolve 0.2g hemin in 20ml ethanol, stir at room temperature for 20min, add 0.7g 2-methylimidazole as an organic ligand, and react for 4h;

[0029] 2) centrifuging the product obtained in step 1), washing with ethanol to obtain a black product, and drying it in an oven at 40° C. to obtain a ZIF-loaded hemin material;

[0030] 3) Load the ZIF-loaded hemin material obtained in step 2) on three melamine sponges, specifically weigh 1.0 g of the ZIF-loaded hemin material in 100 ml of absolute ethanol, To obtain a mixed solution, immerse the melamine sponge material block in the mixed solution, ultrasonically react for 2h, then condense and reflux at 80°C for 4.5h, then centrifuge and wash twice with absolute ethanol at a speed of 300rpm, and place at 60°C Dry under vacuum to obtain a three-dimensional ...

Embodiment 2

[0032] The encapsulation method based on melamine sponge loaded ZIF-hemin provided by the present embodiment comprises the following steps:

[0033] 1) 1.2g Zn(NO 3 ) 2 ·6H 2 O. Dissolve 0.25g of hemin in 20ml of ethanol, stir at room temperature for 30min, add 0.8g of 2-methylimidazole as an organic ligand, and react for 4h;

[0034] 2) centrifuging the product obtained in step 1), washing with ethanol to obtain a black product, and drying it in an oven at 60° C. to obtain a ZIF-loaded hemin material;

[0035] 3) The ZIF-loaded hemin material obtained in the step 2) is loaded on 5 melamine sponges, specifically, 1.2 g of the ZIF-loaded hemin material is weighed and dispersed in 120 ml of absolute ethanol to obtain Mix the solution, immerse the melamine sponge material block in the mixed solution, ultrasonically react for 2.5h, then condense and reflux at 90°C for 5.0h, then centrifuge and wash with absolute ethanol at a speed of 400rpm for 3 times, and vacuum dry at 70°C. ...

Embodiment 3

[0037] The encapsulation method based on melamine sponge loaded ZIF-hemin provided by the present embodiment comprises the following steps:

[0038] 1) 1.5g Zn(NO 3 ) 2 ·6H 2 O. Dissolve 0.3g of hemin in 20ml of ethanol, stir at room temperature for 40min, add 0.9g of 2-methylimidazole as an organic ligand, and react for 4h;

[0039] 2) centrifuging the product obtained in step 1), washing with ethanol to obtain a black product, and drying it in an oven at 80° C. to obtain a ZIF-loaded hemin material;

[0040] 3) The ZIF-loaded hemin material obtained in step 2) is loaded on 7 melamine sponges. The difference between this embodiment and Example 1 is that 1.5g of ZIF-loaded chlorine is weighed in step 3). The heme material is dispersed in 150ml of absolute ethanol to obtain a mixed solution, the melamine sponge material block is immersed in the mixed solution, ultrasonically reacted for 3h, then condensed and refluxed at 100°C for 6h, and then refluxed with absolute ethanol ...

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Abstract

The present invention provides a ZIF-hemin encapsulation method based on melamine sponge, comprising the following steps: 1) dissolving metal salts and hemin in ethanol, stirring at room temperature, adding imidazole as an organic ligand, and reacting; 2) ) Centrifuge the product obtained in the step 1), wash it with ethanol to obtain a black product, and dry it in an oven at 40°C to 80°C to obtain a ZIF-loaded hemin material; 3) The step 2) The obtained ZIF-loaded hemin material is loaded on multiple melamine sponges to form a three-dimensional network nanomaterial based on melamine sponge-loaded ZIF-hemin. The synthesis method provided by the invention is simple and easy, can maintain the morphology and crystallinity of the ZIF material, and has excellent catalytic and cycle performance. The resulting novel nanomaterials offer new avenues for encapsulating enzymes.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and in particular relates to a hemin encapsulation method based on a melamine sponge loaded with a ZIF material and an application thereof. Background technique [0002] As a biocatalyst, enzymes have distinct advantages, such as high activity, good specificity, and mild reaction conditions; however, their disadvantages are also distinct, such as poor chemical stability and thermal stability, easy inactivation, difficult separation and purification, high cost, Poor universality. Most of the previous industrial-level enzyme catalysis applications did not directly use isolated and purified enzymes, but used cells containing these enzymes (including genetically engineered bacteria or animal cells, etc.), which caused great limitations. Cell-free biosynthesis technology is a new field of biotechnology. It explores how to use enzyme-based reaction pathways without using living cells. This technolog...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J31/22C08G83/00
CPCB01J31/1691B01J31/1815C08G83/008B01J2531/26B01J2231/70B01J35/40
Inventor 王颖李风亭封涛
Owner TONGJI UNIV
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