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DNA based on FAPalpha and survivin and application of DNA based on FAPalpha and survivin in production of tumor vaccine

A DNA vaccine, DNA sequence technology, applied in the field of tumor DNA vaccine, to achieve the effect of strong anti-tumor activity

Active Publication Date: 2020-05-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no tumor vaccine targeting FAPα and survivin at the same time at home and abroad, but studies have shown that targeting FAPα can significantly improve the anti-tumor effect of other therapies, including immunotherapy, so it is speculated that this strategy is feasible

Method used

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  • DNA based on FAPalpha and survivin and application of DNA based on FAPalpha and survivin in production of tumor vaccine
  • DNA based on FAPalpha and survivin and application of DNA based on FAPalpha and survivin in production of tumor vaccine
  • DNA based on FAPalpha and survivin and application of DNA based on FAPalpha and survivin in production of tumor vaccine

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Experimental program
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Effect test

Embodiment Construction

[0040] 1. Construction and protein expression identification of CpVR-OsFS DNA vaccine.

[0041] The vector used is the eukaryotic expression plasmid VR1012 with the introduction of CpG motif, referred to as CpVR, which contains promoter CMV, intron A, BGH sequence, CpG sequence, kanamycin resistance sequence, a total of 5305bp, see the map figure 1 .

[0042] Using CpVR-OsF and CpVR-OS as templates, the nucleotide sequence OsF and OS fragment SEQ ID NO:1 were obtained by PCR, and PstI / BamHI restriction sites and homologous sequences identical to the two ends of the vector were introduced at both ends For the arm sequence, perform DNA electrophoresis and gel recovery on the target DNA fragment and the vector fragment obtained by PstI / BamHI digestion to obtain the target fragment and vector, use a homologous recombination kit, incubate at 50°C for 15 minutes, transform Escherichia coli, and coat Kanamycin-resistant LB plates were cultured overnight at 37°C. Select positive col...

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Abstract

The invention relates to DNA based on FAPalpha and survivin and application of the DNA based on the FAPalpha and the survivin in production of a tumor vaccine, in particular to a fused sequence, whichcan be secreted and expressed, shown in SEQ ID NO: 1 as shown in the description of an FAPalpha segment and a survivin segment and application of a DNA vaccine constructed on the basis of the sequence shown in SEQ ID NO: 1 as shown in the description in production of a therapeutic tumor vaccine, and belongs to the field of tumor DNA vaccines. According to the fused sequence, which can be secretedand expressed, shown in SEQ ID NO: 1 as shown in the description of the FAPalpha segment and the survivin segment and application of the DNA vaccine constructed on the basis of the sequence shown inSEQ ID NO: 1 as shown in the description in production of the therapeutic tumor vaccine, through an anti-tumor experiment, it is determined that compared with an individual FAPalpha vaccine and an individual survivin vaccine, the DNA vaccine has higher anti-tumor activity, and shows excellent anti-tumor effects on a mice breast cancer model and a mice pancreatic cancer model, so that the sequenceshown in SEQ ID NO: 1 as shown in the description has better application prospects in production of various tumor vaccines.

Description

technical field [0001] The invention relates to the field of tumor DNA vaccines. Specifically, the present invention relates to the application of the recombinant DNA carrier CpVR-OsFS targeting FAPα and survivin in the preparation of anti-tumor vaccines. Background technique [0002] Cancer has become a major disease that seriously endangers human health. At present, the main methods of tumor treatment include surgery, chemotherapy and radiotherapy. However, traditional treatment methods will cause irreversible physical trauma and secondary physical and psychological damage to patients, and they cannot completely cure cancer. Therefore, there is an urgent need for new cancer treatment methods to relieve pain and improve the quality of life of patients, and ultimately achieve a cure. In recent years, the clinical success of immune checkpoint blockade therapy represented by PD-1 and PD-L1 has opened a new era of cancer immunotherapy. But like most immunotherapies, the pre...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12N15/62A61K39/00A61K45/06A61K31/704A61K31/7068A61P35/00
CPCC12N15/85C07K14/4747C12N9/6424A61K39/00115A61K39/00116A61K45/06A61K31/704A61K31/7068A61P35/00C12N2800/107C07K2319/00C07K2319/02A61K2039/53A61K2300/00
Inventor 张海红于湘晖孔维耿飞郭杰
Owner JILIN UNIV
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