Temperature-sensitive coacervation layer type hyperbranched polyethylene glycol, and preparation method and application thereof

Abstract

The invention discloses temperature-sensitive coacervation layer type hyperbranched polyethylene glycol, and a preparation method and an application thereof, belonging to the field of biomedical materials. The temperature-sensitive coacervation layer type hyperbranched polyethylene glycol disclosed by the invention has large phase transition temperature range by changing of the composition proportion, the molecular weight and the branching degree of the temperature-sensitive coacervation layer type hyperbranched polyethylene glycol. The invention also discloses a preparation method for the temperature-sensitive coacervation layer type hyperbranched polyethylene glycol. The temperature-sensitive coacervation layer type hyperbranched polyethylene glycol is prepared from polyethylene glycol diacrylate, diethylene glycol diacrylate, a chain transfer agent and an initiator through reversible addition-fragmentation chain transfer free radical polymerization under an anaerobic condition. Thepreparation method is simple and highly-efficient, uses cheap and easily-available raw materials, and well reduces the cost of production. Meanwhile, the temperature-sensitive coacervation layer typehyperbranched polyethylene glycol and sulfhydrylated gelatin are subjected to a cross-linking reaction to prepare an injectable hydrogel. The temperature-sensitive coacervation layer type hyperbranched polyethylene glycol has good biocompatibility, so the temperature-sensitive coacervation layer type hyperbranched polyethylene glycol can be applied to the fields of medicine / protein purification, delivery, single cell wrapping, injectable tissue engineering hydrogel stents and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a temperature-sensitive coacervate type hyperbranched polyethylene glycol and a preparation method and application thereof. Background technique [0002] Because coacervate polymers are not completely dehydrated, they will not cause significant changes in molecular conformation, so they have a wide range of applications in the fields of biomaterials, for example, they can be used to wrap biological macromolecules such as proteins without damaging their biological activity. Layered polymers are used as purification reagents for proteins and nucleic acids, controllable delivery vehicles for sensitive physiologically active molecules, and injectable tissue engineering scaffolds. For example, the current purification of proteins and nucleic acids mainly relies on electrophoresis and chromatographic column methods. The process is relatively cumbersome and the efficiency is low. Howev...

AI Technical Summary

Problems solved by technology

[0004] At present, the coacervate polymers are mainly of the coil-globule type, such as specific types of elastin-like polypeptides (ELPs), while the temperature-sensitive coacervate polymers are relatively rare, and only a few The temperature-sensitive coacervate-type polymerization process of polyesters is relatively complicated. It needs to carry out multi-step organic chemical reactions to synthesize monomers, and then carry out polymerization to obtain products.

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