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Method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn

A technology for rapid determination of mycotoxins, applied to measuring devices, instruments, scientific instruments, etc., to achieve the effects of improving efficiency, ensuring quality and safety, and shortening extraction time

Active Publication Date: 2020-05-12
INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

However, these detection methods are only suitable for some traditional mycotoxins, such as aflatoxins B1, B2, G1, G2, fumonisins B1, B2, B3
[0006] At present, there is no detection method for the simultaneous determination of corn pesticide residues and toxins in China, and the relevant detection research is mostly focused on the determination of aflatoxins or pesticides in grains and oils

Method used

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  • Method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn
  • Method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn
  • Method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn

Examples

Experimental program
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Embodiment 1

[0030] 1 Materials and methods

[0031] 1.1 Materials and reagents

[0032] Standards of aflatoxins B1, B2, G1, G2, ochratoxin A and zearalenone were purchased from SigmaAldrich, USA, fumonisins B1, B2, B3, deoxynivalenol, 3-acetyldeoxynivalenol Standards of enol, 15-acetyldeoxynivalenol, T-2 toxin, and penicillin were purchased from Romer Labs, QuEChERS extraction kit (10 g, Agilent). The chemicals and solvents used were of ACS grade, and the methanol, acetonitrile and formic acid used for HPLC analysis were of HPLC grade. Pesticides imidacloprid, acetamiprid, carbendazim, chlorpyrifos, thiamethoxam, difenoconazole, tebuconazole, pirimicarb, triadimefon, propiconazole, prochloraz, fipronil, triazophos , carbofuran, phoxim, isoprocarb, isocarbophos, buprofezin, 3-hydroxy carbofuran, azoxystrobin, methamidophos, pymetrozine, chlorantraniliprole, clothianidin , trifloxystrobin and dimethoate standard were purchased from First Standard Company (Tianjin, China). Pesticide stoc...

Embodiment 2

[0070] Embodiment 2: the mensuration of actual sample

[0071] The method provided in Example 1 was used to measure the residues of 14 mycotoxins and 26 pesticides in 70 corn samples collected from the Shandong area, the contamination rate of mycotoxins and pesticides, and the average and maximum values ​​of positive samples are shown in the table 5. The results showed that 6 kinds of mycotoxins and 4 kinds of pesticides were detected in 70 corn samples, and the pollution rate could reach 100% (carbendazim), followed by fumonisins B1 and B2 (65.7%), fumonisins B3 ( 54.1%), aflatoxin B1 was detected in 16 samples, and the maximum concentration could reach 113.0 μg / kg. my country has set a limit of 20 μg / kg for aflatoxin B1 in corn, and the exceeding rate has reached 7.1%.

[0072] Table 5 Test results of 70 corn samples in Shandong area

[0073]

[0074]

[0075] The above results show that the level of mycotoxin pollution in corn is relatively high, and mycotoxin poll...

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Abstract

The invention discloses a method for rapidly determining 14 mycotoxins and 26 pesticide residues in corn. According to the method of the invention, an extracting solution is adopted for extraction, the ratio of acetonitrile, water, and acetic acid in the extracting solution are 79: 20: 1; an QuEChERS extraction bag (containing MgSO4, NaCl, sodium citrate and disodium citrate) is used for purification; a UPLC-MS / MS method is adopted; a UPLC HSS T3 column is used as a chromatographic column; 0.2% formic acid water / methanol is used as a mobile phase so as to perform qualitative and quantitative analysis; the average recovery rate of all mycotoxins and pesticides ranges from 73.37% to 105.87%. The method is quite suitable for the detection of mycotoxins and pesticides in corn and has importantsignificance in guaranteeing the quality safety of corn products and maintaining human health.

Description

technical field [0001] The invention relates to a method for rapidly determining 14 kinds of mycotoxins and 26 kinds of pesticide residues in corn in combination with solid-phase extraction and UPLC-MS / MS, belonging to the technical field of agricultural product detection. Background technique [0002] Corn is an important grain and industrial processing raw material in my country, and is also the main source of nutrition for animal feed; it is the second largest grain crop in Shandong Province, with a planting area of ​​more than 56 million mu, and is an important feed and industrial processing raw material. By 2013, maize had become the first major grain crop to surpass rice in yield (Food and Agriculture Organization of the United Nations 2013). The quality and safety of corn is related to food safety, human and animal health, and is the lifeline of the corn and feed industry. It also especially affects the high-quality development of animal husbandry and industrial indus...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/7266G01N30/8637G01N30/8679G01N2030/062Y02P60/87
Inventor 梁京芸赵善仓范丽霞苑学霞董燕婕王磊
Owner INST OF QUALITY STANDARDS & TESTING TECH FOR AGRO PROD OF SHANDONG ACADEMY OF AGRI SCI
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