A double-template molecular imprinting purification method for tetrandrine A and B
A technology of molecular imprinting and purification method, which is applied in the field of tetrandrine and tetrandrine, can solve the problems of cumbersome purification steps and achieve the effects of improving safety, promoting development and application, and high loss
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Embodiment 1
[0027] A double-template molecular imprinted purification method for tetrandrine A and B comprises the following steps:
[0028] 1) Preparation of double-template molecularly imprinted polymer: Weigh 40 mmol of tetrandrine and B, mix with 160 mmol of methacrylic acid, place in a stoppered Erlenmeyer flask, and use a mixed solvent of toluene and acetonitrile at a volume ratio of 1:1 Dissolve, seal, and vibrate in a shaker for 4 hours to fully react; add 800mmol ethylene glycol dimethacrylate and 480mmol azobisisobutylcyanide, shake well, transfer the solution into a glass ampoule, and blow nitrogen for 20 minutes to remove Oxygen; react in a constant temperature water bath at 60°C for 40 hours; filter the generated polymer to remove the solvent, cool to room temperature, grind, and pass through a 100-mesh sieve; pack the sieved polymer into a column, and use 70% ethanol aqueous solution to elute the template molecules and untreated polymers. Reaction or excess functional monome...
Embodiment 2
[0032] A double-template molecular imprinted purification method for tetrandrine A and B comprises the following steps:
[0033] 1) Preparation of double-template molecularly imprinted polymer: Weigh 40 mmol of tetrandrine and B, mix with 120 mmol of methacrylic acid, place in a stoppered Erlenmeyer flask, and use a mixed solvent of toluene and acetonitrile at a volume ratio of 1:5 Dissolve, seal, shake in a shaker for 1 hour to fully react; add 1000mmol ethylene glycol dimethacrylate and 600mmol azobisisobutylcyanide, shake well, transfer the solution into a glass ampoule, blow nitrogen for 5 minutes to remove Oxygen; react in a constant temperature water bath at 40°C for 48 hours; filter the generated polymer to remove the solvent, cool to room temperature, grind, and pass through a 50-mesh sieve; pack the sieved polymer into a column, and use 50% ethanol aqueous solution to elute the template molecules and untreated polymers. Reactive or excess functional monomers, the elut...
Embodiment 3
[0037] A double-template molecular imprinted purification method for tetrandrine A and B comprises the following steps:
[0038] 1) Preparation of double-template molecularly imprinted polymer: weigh 40 mmol of tetrandrine and B, mix with 200 mmol of methacrylic acid, place in a stoppered Erlenmeyer flask, use a mixed solvent of toluene and acetonitrile with a volume ratio of 1:3 Dissolve, seal, and vibrate in a shaker for 2 hours to fully react; add 600mmol ethylene glycol dimethacrylate and 400mmol azobisisobutylcyanide, shake well, transfer the solution into a glass ampoule, and blow nitrogen for 15 minutes to remove Oxygen; react in a constant temperature water bath at 50°C for 30 hours; filter the generated polymer to remove the solvent, cool to room temperature, grind, and pass through a 100-mesh sieve; pack the sieved polymer into a column, and use 80% ethanol aqueous solution to elute the template molecules and untreated polymers. Reaction or excess functional monomers...
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