Process for extracting casing heparin sodium in enzymolysis mode

Abstract

The invention relates to the field of heparin sodium crude product preparation, and particularly relates to a process for extracting casing heparin sodium in an enzymolysis mode. The process comprisesa treatment stage, a primary filtration stage, a treatment stage, a secondary filtration stage, an adsorption stage, a production stage and an elution stage. The beneficial effects are that: the primary filtration stage and the secondary filtration stage are adopted, therefore, the heparin sodium in a filtrate is completely separated out and retained; then, in the adsorption stage, a resin is soaked with a sodium chloride solution, heparin sodium is separated out of the resin, and meanwhile, alcohol can be added, so that after the heparin sodium is separated out of the sodium chloride solution, precipitate is naturally generated under the action of the alcohol, the later production time of the heparin sodium is effectively prolonged, and the processing efficiency is improved.

Description

technical field [0001] The invention relates to the field of preparation of crude heparin sodium, in particular to a process for extracting intestinal-coated heparin sodium through enzymatic hydrolysis. Background technique [0002] Heparin sodium (Heparin sodium) can interfere with many links in the blood coagulation process, and has anticoagulant effects in vivo and in vitro. Its mechanism of action is relatively complicated, mainly through the combination with antithrombin III (AT-III). [0003] Heparin sodium is often extracted from the small intestinal mucosa of animals. The traditional small intestinal mucosal extraction method takes a relatively long time to extract heparin sodium in the small intestine, the extraction rate of heparin sodium is low, and the processing steps are cumbersome. The precipitation time is long, which affects the preparation time, and there is an improvement. Contents of the invention [0004] The purpose of the present invention is to pro...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a process for extracting casing heparin sodium by enzymatic hydrolysis, so as to solve the problem of relatively long extraction time, low extraction rate of heparin sodium, cumbersome processing steps and the problem of heparin sodium in alcohol solution in the later stage when extracting traditional heparin sodium. The precipitation time is long, which affects the preparation time