Process for extracting casing heparin sodium in enzymolysis mode

An enteric-coated heparin sodium and enzymatic hydrolysis technology, which is applied in the field of extracting enteric-coated heparin sodium by enzymatic hydrolysis, can solve the problems of affecting preparation time, long extraction time, and long precipitation time of heparin sodium, and achieve the goal of improving production time and processing efficiency Effect

Inactive Publication Date: 2020-05-19
霍邱县天泽生物科技有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide a process for extracting casing heparin sodium by enzymatic hydrolysis, so as to solve the problem of relatively long extraction time, low extraction rate of heparin sodium, cumbersome processing steps and the problem of heparin sodium in alcohol solution in the later stage when extracting traditional heparin sodium. The precipitation time is long, which affects the preparation time

Method used

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Embodiment Construction

[0021] The specific implementation of the present invention will be further described below in conjunction with the examples. The following examples are only used to illustrate the technical solution of the present invention more clearly, but not to limit the protection scope of the present invention.

[0022] The specific steps of the production method are as follows:

[0023] ①. Processing stage: clean the small intestine, put it in a container, add 10% sodium chloride by weight of the small intestine, and then add water to completely submerge the small intestine, soak for 30 minutes, and then stir for 15 minutes to form a filtrate.

[0024] ②. Primary filtration stage: Use a gauze filter between 100-150 mesh, introduce the small intestine together with the filtrate into the gauze filter for filtration, and generate the filtrate.

[0025] ③. Processing stage: put the filtrate in a container, add 20%-30% sodium hydroxide, control the pH value between 7-9, then heat it to 45°...

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Abstract

The invention relates to the field of heparin sodium crude product preparation, and particularly relates to a process for extracting casing heparin sodium in an enzymolysis mode. The process comprisesa treatment stage, a primary filtration stage, a treatment stage, a secondary filtration stage, an adsorption stage, a production stage and an elution stage. The beneficial effects are that: the primary filtration stage and the secondary filtration stage are adopted, therefore, the heparin sodium in a filtrate is completely separated out and retained; then, in the adsorption stage, a resin is soaked with a sodium chloride solution, heparin sodium is separated out of the resin, and meanwhile, alcohol can be added, so that after the heparin sodium is separated out of the sodium chloride solution, precipitate is naturally generated under the action of the alcohol, the later production time of the heparin sodium is effectively prolonged, and the processing efficiency is improved.

Description

technical field [0001] The invention relates to the field of preparation of crude heparin sodium, in particular to a process for extracting intestinal-coated heparin sodium through enzymatic hydrolysis. Background technique [0002] Heparin sodium (Heparin sodium) can interfere with many links in the blood coagulation process, and has anticoagulant effects in vivo and in vitro. Its mechanism of action is relatively complicated, mainly through the combination with antithrombin III (AT-III). [0003] Heparin sodium is often extracted from the small intestinal mucosa of animals. The traditional small intestinal mucosal extraction method takes a relatively long time to extract heparin sodium in the small intestine, the extraction rate of heparin sodium is low, and the processing steps are cumbersome. The precipitation time is long, which affects the preparation time, and there is an improvement. Contents of the invention [0004] The purpose of the present invention is to pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0003C08B37/0075
Inventor 胡安国
Owner 霍邱县天泽生物科技有限公司
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