The invention discloses a preparation method of mulberry whole fruit powder, which comprises the following steps: (1) removing impurities from fresh mulberries, conducting cleaning and pulping, and carrying out enzymolysis on endogenous pectin; (2) adding a softener, stirring, dissolving, dispersing and softening the mulberry seeds, conducting heating for degasing, conducting sterilization, deactivating enzyme and softening the mulberry seeds by heating power; (3) refining the slurry by a colloid mill for 2-3 times to refine the mulberry seed particles and release the mulberry seed oil, adding citric acid fatty acid glyceride, conducting stirring, dispersing and dissolving, carrying out micronization treatment for 1-2 times by using a colloid mill to obtain emulsified mulberry pulp, and using a homogenizer for carrying out particle ultrafine pulverization, so as to obtain slurry homogenized liquid; and (4) performing spray drying and cooling, adding an anti-caking agent, and performing uniform mixing so as to obtain the mulberry whole fruit powder product. According to the method, the mulberry pulp and the mulberry seeds are completely utilized, the raw material utilization rate is high, the nutritional value is high, and the product flavor is pure.