Normal-temperature preservation method of nitrifying bacteria

A technology of nitrifying bacteria and normal temperature, which is applied in the field of normal temperature preservation of nitrifying bacteria, and achieves the effect of long storage period at normal temperature, good preservation activity and low preservation cost

Active Publication Date: 2022-07-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method also requires the addition of a preserved nutrient solution of a specific composition

Method used

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  • Normal-temperature preservation method of nitrifying bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The microalgae used in this example is the chlorella disclosed in CN109576158A ( Chlorella sp. ), the deposit number is CGMCC No.11005. When the microalgae was cultured to a concentration of 5 g / L, the algal cells were collected by filtration.

[0034] (1) The nitrifying bacteria were aerated and cultivated to the stable growth stage. The cultivation conditions were as follows: the temperature was 25 °C, the pH was 7.0 to 7.5, and the dissolved oxygen concentration was 2-3 mg / L. After the cultivation was stopped in the stable growth stage, the bacteria were collected by filtration.

[0035] (2) Add the bacterial cells and microalgae cells to the preservation solution. The bacterial cells are added according to the mass ratio of the bacterial mass to the culture solution as 5:100, and the amount of the microalgae cells is 5% of the nitrifying bacteria cell mass. , the preservation conditions are: 20 ℃, alternating light and dark culture, the total time of sunlight is 12...

Embodiment 2

[0038] The microalgae used in this embodiment is Pseudochlorella kjeldahl disclosed in CN106467896A ( Parachlorella kessleri ) FSH-Y3, deposit number CGMCC No.9238. When the microalgae was cultured to a concentration of 5 g / L, the algal cells were collected by filtration.

[0039] (1) The nitrifying bacteria were aerated and cultivated to the stable growth stage. The cultivation conditions were as follows: the temperature was 25 °C, the pH was 7.0 to 7.5, and the dissolved oxygen concentration was 2-3 mg / L. After the cultivation was stopped in the stable growth stage, the bacteria were collected by filtration.

[0040] (2) Add the bacterial cells and microalgae cells to the preservation solution. The bacterial cells are added according to the mass ratio of the bacterial mass to the culture solution as 10:100, and the amount of the microalgal cells is 3% of the nitrifying bacteria mass. , the preservation conditions are: 15 ℃, alternating light and dark culture, the total tim...

Embodiment 3

[0043] The microalgae used in this example is the fibrous algae disclosed in CN105713836A ( Ankistrodesmus sp. ) SS-B7, the deposit number is CGMCC No.7478. When the microalgae were cultured to a concentration of 6 g / L, the algal cells were collected by filtration.

[0044] (1) The nitrifying bacteria were cultured by aeration to the stable growth stage. The culture conditions were as follows: the temperature was 15 °C, the pH was 7.0 to 7.5, and the dissolved oxygen concentration was 2-3 mg / L. The cultured to the stable growth stage, and the bacteria were collected by filtration.

[0045] (2) Add the bacterial cells and microalgae cells to the preservation solution. The bacterial cells are added according to the mass ratio of the bacterial mass to the culture solution as 20:100, and the amount of the microalgae cells is 10% of the nitrifying bacteria mass. , the preservation conditions are: 25 ℃, alternating light and dark culture, the total time of sunlight is 14h, and the...

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Abstract

The invention relates to a normal-temperature preservation method of nitrifying bacteria, which comprises the following steps: (1) carrying out aeration culture on the nitrifying bacteria to a stable growth period, and collecting thalli; (2) adding the thalli and the microalgae into a preservation solution, and preserving the thalli and the microalgae; the preservation condition is room temperature, light and dark alternate culture is carried out, and the total sunlight illumination time is longer than 8 h; and (3) after the preservation is finished, cutting out an upper microalgae layer, and adding the rest materials into a culture solution for aeration culture to obtain the nitrifying bacteria with stable activity. According to the invention, large-batch normal-temperature preservation of thalli is realized by adopting a phycomycete symbiosis mode, and the method has the advantages of long normal-temperature preservation period, good preservation activity, high recovery speed and the like.

Description

technical field [0001] The present invention relates to the preservation of nitrifying bacteria, in particular to a normal temperature preservation method of nitrifying bacteria. Background technique [0002] Nitrogen is one of the important factors causing water pollution, and it is mostly removed by biological methods at present. Biological denitrification is to first oxidize ammonia nitrogen to nitrate nitrogen or / and nitrous nitrogen through nitrification reaction, and then reduce nitrate or nitrite nitrogen to gaseous nitrogen through denitrification reaction and remove it from water, mainly relying on nitrifying bacteria and denitrifying bacteria. . Strain is one of the important biological resources. After an excellent strain is selected, it must keep its excellent properties unchanged or slow down as little as possible, so as not to reduce the performance of the bacteria, and it can be used in production for a long time. application. [0003] The basic principle o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12C12N1/04C12R1/89
CPCC12N1/20C12N1/12C12N1/04Y02W10/10
Inventor 孙丹凤高会杰陈明翔王刚李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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