Blood cell quick detection method based on surface enhanced Raman

A surface-enhanced Raman and detection method technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of complicated operation process, complicated operation, time-consuming detection, etc., and achieve simple operation method, simple operation process, blood sample little effect

A surface-enhanced Raman and detection method technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of complicated operation process, complicated operation, time-consuming detection, etc., and achieve simple operation method, simple operation process, blood sample little effect

CN111175279APending Publication Date: 2020-05-19XUZHOU NORMAL UNIVERSITY

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  • Blood cell quick detection method based on surface enhanced Raman
  • Blood cell quick detection method based on surface enhanced Raman
  • Blood cell quick detection method based on surface enhanced Raman

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Embodiment 1

[0025] Embodiment 1: as figure 1 Shown is a picture of a blood sample under a microscope. This embodiment provides a method for rapid detection of red blood cell structure provided by the present invention, which includes the following steps:

[0026] Step 1, using a dynamic shadow deposition method to prepare a silver nanorod array substrate. Cut the glass sheet into 1cm×1cm, add ethanol and clean it in an ultrasonic cleaner for 5min, then dry it with nitrogen gas, and fix it in the deposition chamber; when the pressure is less than 5×10 -7 In the Torr vacuum deposition chamber, through electron beam evaporation, a layer of titanium thin film and silver thin film were successively deposited on the cleaned glass at the speed of 0.2nm / s and 0.3nm / s, and then the sample stage was rotated so that the normal line of the substrate was in line with the The included angle of the deposition direction is 86 °, and a 900nm thick silver nanorod array is grown at a speed of 0.3nm / s by el...

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Abstract

The invention discloses a blood cell quick detection method based on surface enhanced Raman. The method is based on a surface enhanced Raman spectroscopy (SERS) detection technology. According to themethod, a clinical blood sample is quickly diagnosed and analyzed, the component and structure change of the detected erythrocyte can be quickly judged, the sensitivity of the detection method is higher and more stable, the influence of the external temperature and the sample is greatly reduced, the detection is more convenient, the single-time measurement cost is lower, and the measurement cost is greatly saved.

Description

technical field [0001] The invention relates to a rapid detection method of blood cells based on surface-enhanced Raman, in particular to a method for measuring the structure of red blood cells. Background technique [0002] Erythrocytes, also known as red blood cells, are the most numerous blood cells in the blood. They are also the main medium for transporting oxygen through the blood in vertebrates, and they also have immune functions. Red blood cells release energy by breaking down glucose. transport oxygen and some carbon dioxide. It is dark purple when transporting carbon dioxide and bright red when transporting oxygen. In addition, erythrocytes have the following immune functions: identify carrying antigens; clear immune complexes in circulation; enhance T cell-dependent responses; promote phagocytosis, etc. [0003] At present, the detection method of red blood cells, that is, blood routine examination, needs to dilute the red blood cells into Hayem's solution. Th...

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Application Information

Patent Timeline
19 May 2020
Publication
CN111175279A
IPC
G01N21/65
CPC
G01N21/658
Inventors
田康振; 聂新明