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Blood cell quick detection method based on surface enhanced Raman

A surface-enhanced Raman and detection method technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of complicated operation process, complicated operation, time-consuming detection, etc., and achieve simple operation method, simple operation process, blood sample little effect

Pending Publication Date: 2020-05-19
XUZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection method of red blood cells, that is, blood routine examination, needs to dilute the red blood cells into Hayem's solution. This kind of method is not only complicated to operate, but also has high requirements on the quality of the personnel who operate the instrument and the performance of the instrument itself.
And detection time-consuming, high operating costs
Surface-enhanced Raman and the traditional detection method, the SERS substrate needs to use the chemical reduction method to prepare gold or silver sol. The control is difficult to accurately quantify, resulting in inconsistent particle size and dispersion of gold and silver nanoparticles prepared each time, and poor reproducibility of the obtained substrate
When using a liquid SERS substrate, the prepared nanoparticles need to be cleaned repeatedly, the operation process is complicated, and the detection is easily affected by the external temperature and the properties of the sample itself, making it difficult to perform qualitative and quantitative detection.
[0004] At present, with regard to the detection of red blood cells, there are problems such as high detection cost, long cycle, and only a single component can be detected at a time.

Method used

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  • Blood cell quick detection method based on surface enhanced Raman
  • Blood cell quick detection method based on surface enhanced Raman
  • Blood cell quick detection method based on surface enhanced Raman

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Embodiment 1

[0025] Embodiment 1: as figure 1 Shown is a picture of a blood sample under a microscope. This embodiment provides a method for rapid detection of red blood cell structure provided by the present invention, which includes the following steps:

[0026] Step 1, using a dynamic shadow deposition method to prepare a silver nanorod array substrate. Cut the glass sheet into 1cm×1cm, add ethanol and clean it in an ultrasonic cleaner for 5min, then dry it with nitrogen gas, and fix it in the deposition chamber; when the pressure is less than 5×10 -7 In the Torr vacuum deposition chamber, through electron beam evaporation, a layer of titanium thin film and silver thin film were successively deposited on the cleaned glass at the speed of 0.2nm / s and 0.3nm / s, and then the sample stage was rotated so that the normal line of the substrate was in line with the The included angle of the deposition direction is 86 °, and a 900nm thick silver nanorod array is grown at a speed of 0.3nm / s by el...

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Abstract

The invention discloses a blood cell quick detection method based on surface enhanced Raman. The method is based on a surface enhanced Raman spectroscopy (SERS) detection technology. According to themethod, a clinical blood sample is quickly diagnosed and analyzed, the component and structure change of the detected erythrocyte can be quickly judged, the sensitivity of the detection method is higher and more stable, the influence of the external temperature and the sample is greatly reduced, the detection is more convenient, the single-time measurement cost is lower, and the measurement cost is greatly saved.

Description

technical field [0001] The invention relates to a rapid detection method of blood cells based on surface-enhanced Raman, in particular to a method for measuring the structure of red blood cells. Background technique [0002] Erythrocytes, also known as red blood cells, are the most numerous blood cells in the blood. They are also the main medium for transporting oxygen through the blood in vertebrates, and they also have immune functions. Red blood cells release energy by breaking down glucose. transport oxygen and some carbon dioxide. It is dark purple when transporting carbon dioxide and bright red when transporting oxygen. In addition, erythrocytes have the following immune functions: identify carrying antigens; clear immune complexes in circulation; enhance T cell-dependent responses; promote phagocytosis, etc. [0003] At present, the detection method of red blood cells, that is, blood routine examination, needs to dilute the red blood cells into Hayem's solution. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 田康振聂新明田亚平
Owner XUZHOU NORMAL UNIVERSITY
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