High performance liquid chromatography method for determining soluble sugar in tomato fruits

A high-performance liquid chromatography and soluble technology, which is applied in the field of high-performance liquid chromatography for the determination of soluble sugars in tomato fruits, can solve problems such as the inability to detect multiple soluble sugars at the same time

Inactive Publication Date: 2020-05-19
SHENZHEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-performance liquid chromatography method for measuring soluble sugar in tomato fruit, aiming to solve the problem that high-performance liquid chromatography cannot simultaneously detect multiple soluble sugars in the prior art

Method used

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  • High performance liquid chromatography method for determining soluble sugar in tomato fruits
  • High performance liquid chromatography method for determining soluble sugar in tomato fruits
  • High performance liquid chromatography method for determining soluble sugar in tomato fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Preparation of Standard Curve for Standards

[0072] Prepare the mixed standard solution of glucose standard, sucrose standard and fructose standard: accurately weigh the appropriate amount of glucose, sucrose and fructose respectively, and use Wahaha purified water to make a mixed standard solution containing 50 mg of glucose, sucrose and fructose per 1 mL. The mixed standard solution was filtered using a 0.45 μm filter membrane.

[0073] Prepare the mixed standard diluent: dilute the mixed standard solution step by step to prepare 6 mixed standard diluents with different concentrations, the 6 mixed standard diluents include 30mg / mL mixed standard diluent, 20mg / mL / mL mixed standard diluent, 10mg / mL mixed standard diluent, 5mg / mL mixed standard diluent, 2.5mg / mL mixed standard diluent, 1.25mg / mL mixed standard diluent.

[0074] High-performance liquid chromatography series RID-20A differential refractive index detector analysis: the standard spectrum is obtained by de...

Embodiment 2

[0077] Determination of Soluble Sugar Content in Tomato at Different Development Stages

[0078] Prepare tomato samples: take 4 tomato samples at different development stages, separate the peel and pulp, and remove the inner seeds of the pulp, grind the pulp with liquid nitrogen into powder; add 0.1g of the sample to a 15mL centrifuge tube , accurately weighed in a test tube, recorded the fresh weight of the sample, 1mL 80% ethanol, mixed evenly, kept the extract in a water bath at 35°C for 30min, centrifuged at 12000rcf for 20min at room temperature, and took the supernatant in another centrifuge tube. The fruit sample was extracted twice with 80% ethanol, the extract was collected, centrifuged and concentrated to dryness, the residue was dissolved in 200 μL Wahaha purified water, filtered through a 0.45 μm filter membrane, and ultrasonically degassed to prepare 4 different Tomato test samples in developmental stages; 3 different samples were taken in each stage, and 3 groups...

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Abstract

The invention provides a high performance liquid chromatography method for determining soluble sugar in tomato fruits. The method is characterized in that a high performance liquid chromatography is connected with an RID-20A differential refraction detector in series to detect the soluble sugar, glucose and fructose which are both pentose can be distinguished, the effect of accurately detecting three soluble sugars including glucose, fructose and cane sugar at the same time is achieved, the needed sample amount is small, and accurate detection can be conducted with only 0.1 g or even less samples. The method has advantages of few detection steps, simplicity, rapidness, good reproducibility, reduction of experiment errors, improvement of the accuracy of data, simultaneous qualitative and quantitative determination of three soluble sugars comprising glucose, fructose and sucrose, and provision of a scientific basis for accurate determination and rapid detection of mixture of the solublesugars in tomatoes.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a high-performance liquid chromatography method for determining soluble sugar in tomato fruit. Background technique [0002] Tomato is an annual or perennial plant belonging to Solanaceae (Solanaceae) and is a vegetable economic crop with high nutritional value. It is widely planted in the north and south of my country. Because of its sweet and sour taste and rich nutrition, it is deeply loved by consumers. [0003] The taste of tomato is determined by many factors, and the taste of tomato is affected by many factors, and soluble sugar is one of the important determinants. The main soluble sugar components in tomato are glucose, fructose and sucrose, of which the content of glucose and fructose is more. As the tomato fruit ripens, the content of soluble sugar in the tomato will change, which in turn will affect the flavor of the tomato. [0004] The current analysis of solu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14G01N30/86G01N30/06
CPCG01N30/02G01N30/04G01N30/14G01N30/8631G01N2030/042G01N2030/146
Inventor 黄腾波邱琦芸李紫薇
Owner SHENZHEN UNIV
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