Culture box special for culturing cordyceps militaris

Abstract

The invention relates to a culture box special for culturing cordyceps militaris. The culture box comprises a culture box body; a sealing film is clamped and fixed at the top of the culture box body through a clamping hoop; a top cover is clamped and mounted on the clamping hoop; ventilation holes are formed in the top cover; a sterile respirator component is mounted at the position of each ventilation hole; and handles are mounted at the top of the top cover. The culture box can be used for culturing the cordyceps militaris, and can be sterilized by match of a modern vacuum sterilization technology without influencing performance of a plastic film. In addition, the structure can be suitable for culturing with low oxygen demand in the initial stage, and also can be suitable for culturing requirements of large oxygen demand and high light transmittance in the later stage.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation equipment, in particular to a special cultivation box for cultivation of Cordyceps militaris. Background technique [0002] In the cultivation process of Cordyceps militaris, cultivation box is needed. In order to prevent the infection or contamination of miscellaneous bacteria, it is necessary to effectively seal the cultivation box and then sterilize it for inoculation and cultivation. However, different requirements need to be met during the cultivation process. Oxygen supply requirements, at present, the usual method is to cover the top of the culture box directly with a plastic film in the early stage, and use a rubber band to tighten the periphery of the plastic film to seal and sterilize the culture box. Carry out puncture to realize oxygenation, adopt the above-mentioned method to have the following several problems: [0003] (1) Since the entire culture box needs to be ...

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Problems solved by technology

[0003] (1) Since the entire culture box needs to be sterilized at high temperature before inoculation and culture, the culture environment also needs to be frequently sterilized in space, and rubber bands are used to tighten and seal the plastic film, so multiple high-temperature sterilization or ultraviolet disinfection are easy The aging of the rubber band will lead to insufficient tightening force, which will affect the subsequent use;

[0004] (2) In order to create the airtight conditions required for the sterilization of the culture material, it is necessary to fix the complete film on the mouth of the culture box with a rubber band (in the initial culture, the demand for oxygen is relatively low, so only a few holes are reserved on the film. A small number of air holes), so that during high temperature sterilization, especially vacuum sterilization, it is easy to cause deformation or even bursting of the plastic film, which affects subsequent use;

[0005] (3) For the later stage of cultivation, the demand for oxygen and light transmittance is relatively high, so a lot of air holes need to be pierced on the film. At present, the post-stage piercing is mainly done manually. The operation process is cumbersome and the workload is heavy;

[0006] (4) The above-mentioned plastic film cannot be used again after being used once. On the one hand, it causes environmental pollution, and on the other hand, it also causes a waste of resources. Convenience, low work efficiency

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