PCR identification method of soybean rhizobium strain 5873, and primers and application of method

A technology of 5873PCR and soybean rhizobia, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problems of unfavorable production strain intellectual property protection, cumbersome process, difficult to distinguish, etc., to avoid A large number of sequencing work and the effect of accurate detection
CN111235291AActive Publication Date: 2020-06-05INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Publication Date
2020-06-05

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Abstract

The invention relates to a PCR identification method of soybean rhizobium strain 5873, and primers and application of the method, and belongs to the technical field of strain identification. The method comprises the following steps: using the liquid after lysis, boiling water bath and freeze-thaw of the genomic DNA of strains to be tested or pure culture liquid of the strains or a root nodule crushed extract as templates, and performing PCR amplification using primers 4-4; performing electrophoresis of the PCR products, and identifying that the samples without bands around 355 bp are not the soybean rhizobium strain 5873; continuing PCR amplification of the strains to be test by using a primer Q1, when bands around 355 bp exist; and performing electrophoresis of the above products, and identifying that the samples with bands around 218 bp are the soybean rhizobium strain 5873. According to the present invention, the method has the following advantages that the soybean rhizobium strain5873 can be distinguished simply and quickly in terms of strain identification and evaluation, and bacterial bodies or root nodules can be directly used as templates, so that the tedious steps such asseparation, culture and DNA extraction and identification root nodules are omitted.
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Description

technical field

[0001] The invention belongs to the technical field of strain identification, and in particular relates to a PCR identification method of soybean rhizobium strain 5873, primers used therein and applications. Background technique

[0002] With the adjustment of the country's agricultural strategy, the development of resource-saving and environment-friendly "two-type agriculture" has become the only way for the sustainable development of modern agriculture. Among them, microbial fertilizers including rhizobia, as new green inputs and priority biological products, can not only repair and improve soil, reduce weight and increase efficiency, but also perform outstandingly in improving crop quality and efficiency. The action plan of reducing one increase provides an important guarantee for the realization of the goal of "one control, two reductions and three basics".

[0003] As an important functional strain in microbial fertilizer products, soybean rhizobia can ...

Claims

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