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Method for determining decyl methyl ammonium chloride in plant

A decamethonium chloride and plant technology, applied in the field of plant detection, can solve the problems of reducing the molecular ionization ratio, increasing the risk of target loss, poor sensitivity and stability, etc. reliable results

Active Publication Date: 2020-06-05
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cao H W used water-methanol mixed solvent as the extractant and carried out solid-phase extraction with weak cation exchange columns, and established an analysis method for five kinds of quaternary ammonium salts in apples, spinach and rice; this method was applied to the detection of decyl ammonium chloride There are three problems: one is that the pH of the extract is not adjusted, which reduces the ratio of molecular ionization, and the recovery stability of the sample is questionable; the other is the sample concentration process. Due to the physical and chemical properties of methanol itself, low-temperature concentration will take time. Longer, increasing the risk of loss of the target; third, using GC-MS analysis, the sensitivity and stability of the instrument are worse than UPLC-MS / MS method

Method used

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  • Method for determining decyl methyl ammonium chloride in plant
  • Method for determining decyl methyl ammonium chloride in plant
  • Method for determining decyl methyl ammonium chloride in plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The mensuration of decyl ammonium chloride in the rice straw of embodiment 1

[0041] The harvested Nanjing 5055 rice stalks were selected, and the decyl ammonium chloride was determined. The rice straw was collected from a test base in Jiangning, Nanjing, and the soil and weeds adhering to it were removed, pre-frozen in the refrigerator for 24 hours, and then the sample was placed in a vacuum freeze dryer, freeze-dried at -70°C and vacuum degree of 100Pa Until the water in the rice stalk is completely removed. Manually cut and grind the sample, weigh 5g / part in the Erlenmeyer flask for later use.

[0042]Prepare an acidic organic extraction solution in the quantitative tube, accurately pipette 15mL of acetonitrile / part and 0.10mL of formic acid / part into the quantitative tube, make up to 20mL with ultrapure water, fully mix the solution, pour it into the sample, and Ultrasonic extraction was performed at 25° C. at an ultrasonic frequency of 90 kHz for 18 min; the mix...

Embodiment 2

[0048] The mensuration of decyl ammonium chloride in embodiment 2 ryegrass

[0049] A typical forage grass in my country - ryegrass was selected as the test plant, and the decyl ammonium chloride was determined. The ryegrass was collected from a planting base in Jiangning, Nanjing. The adhered soil and rotten individuals were manually removed, and the samples were pre-frozen in the refrigerator for 24 hours. Then, the samples were placed in a vacuum freeze dryer and freeze-dried at -70°C and a vacuum of 10Pa. Until the moisture in the grass sample is completely removed, manually cut and grind the sample, and weigh 5g / part in the Erlenmeyer flask for later use.

[0050] Prepare the acidic organic extraction solution in the quantitative tube: accurately pipette 15mL of acetonitrile / part and 0.10mL of formic acid / part into the quantitative tube, make up to 20mL with ultrapure water, fully mix the solution, and pour it into the sample. Ultrasonic extraction was performed at 25° C...

Embodiment 3

[0055] The mensuration of decyl ammonium chloride in embodiment 3 Haiqing

[0056] A typical vegetable variety in the Yangtze River Basin of my country-Shanghaiqing was selected as the test plant, and the decyl ammonium chloride was determined. Shanghai Green is collected from a vegetable base in Jiangning, Nanjing. Remove the soil and weeds adhering to it, pre-freeze in the refrigerator for 24 hours, and then put the sample in a vacuum freeze dryer, freeze-dry at -70°C, vacuum degree 1Pa until all the moisture in the sample was removed. Cut and grind the sample manually, and weigh 5g / part in the Erlenmeyer flask for later use.

[0057] Prepare the acidic organic extraction solution in the quantitative tube: accurately pipette 15mL of acetonitrile / part and 0.10mL of formic acid / part into the quantitative tube, make up to 20mL with ultrapure water, fully mix the solution, and pour it into the sample. Ultrasonic extraction was performed at 25° C. for 20 min at an ultrasonic fr...

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Abstract

The invention discloses a method for determining decyl methyl ammonium chloride in a plant. Freeze drying, acidified organic solvent extraction and Florisil soil purification are adopted as a plant sample pretreatment technology, UPLC-MS / MS is adopted as a detection technology of a sample, and the whole operation process is optimized from the aspects of equipment, materials and technologies. The combination technology is used for analyzing decyl methyl ammonium chloride in plants, and has the technical advantages of simplicity, convenience, practicability, accuracy and high selectivity. The method provides an important reference for establishing a standard method for detecting decyl methyl ammonium chloride in plants, provides an important method basis for evaluating the current situationof decyl methyl ammonium chloride residues in economic and edible plant agricultural products in China and organic food authentication work, and can be applied to detection and research of decyl methyl ammonium chloride in various plants.

Description

technical field [0001] The invention relates to the field of plant detection, in particular to a method for determining decyl ammonium chloride in plants. Background technique [0002] Decyl ammonium chloride, also known as didecyl dimethyl ammonium chloride, is a cationic surfactant. Its quaternary ammonium cations can actively attract and accumulate on the surface of negatively charged bacteria and viruses, hindering bacterial metabolism and causing their membrane permeability changes. It can be widely used in various farms and pet clinics for disinfection of the indoor environment, drinking water, animal body surfaces, milk cows and sow udders, breeding eggs, various equipment and utensils, vehicles, and staff. Simultaneously, because decyl ammonium chloride is used in a large amount, causes it to discharge with waste water, waste residue, causes environmental residue and pollution, and decyl ammonium chloride is not easy to degrade in the environment, especially in anae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/146
Inventor 孔祥吉张雪梅田丰许静孔德洋
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA