Lactobacillus reuteri with weight reducing function and application of lactobacillus reuteri

A Lactobacillus reuteri, functional technology, applied in the field of microbiology, can solve problems such as gastrointestinal discomfort, allergies, and decreased plasma concentration of fat-soluble vitamins, and achieve weight loss, fat accumulation, and good acid and bile salt resistance. Effect

Active Publication Date: 2020-06-09
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The only weight loss drug approved in China is orlistat, but it has certain side effects, such a...

Method used

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  • Lactobacillus reuteri with weight reducing function and application of lactobacillus reuteri
  • Lactobacillus reuteri with weight reducing function and application of lactobacillus reuteri
  • Lactobacillus reuteri with weight reducing function and application of lactobacillus reuteri

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Embodiment 1

[0040] The bacterial strain provided by the present invention was identified to belong to Lactobacillus reuteri (Lactobacillus reuteri), named 1723, and was preserved on October 23, 2019 at the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee, Chaoyang, Beijing, China No. 3, No. 1 Yard, Beichen West Road, District, the microbial preservation number is CGMCC NO.18729.

[0041] The bacterial strain Lactobacillus reuteri 1723 provided by the present invention is isolated from ghee collected in the village of Shigatse City, Tibet Autonomous Region of my country.

[0042] The biological properties of bacterial strain Lactobacillus reuteri 1723 of the present invention are as follows:

[0043] Morphological characteristics: In MRS agar medium, the growth form is milky white colony, opaque, round, smooth and moist surface, neat edges, and raised in the center. Gram staining is typically positive, and the cells are short ro...

Embodiment 2

[0051] After the bacterial strain Lactobacillus reuteri 1723 of the present invention, the commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) are activated by the second generation, the bacteria liquid at the end of logarithmic growth is taken, centrifuged at 4000rpm for 10min, and discarded. To obtain the bacteria sludge, carry out the following operations respectively: ①Add the same volume of MRS solution with pH 2.0 and 2.5, mix by pipetting, incubate at 37°C, measure 0 point by dilution coating counting method and incubate for 1h, 2h, Changes in the number of bacteria after 4 hours; ② Add the same volume of MRS solution containing 0.3% bile salts, mix well by pipetting, incubate at 37°C, and measure the number of bacteria at 0 point and after incubation for 4 hours and 8 hours by dilution coating counting method The change. Strain survival rate calculation formula:

[0052] Strain survival rate (%)=N1 / N0*100%.

[0053]...

Embodiment 3

[0059] The HT-29 cell culture system was established, and the cells were grown in DMEM medium containing 10% fetal bovine serum (containing penicillin 100 U / mL, streptomycin 100 mg / mL). After the cells were transferred to the third passage, they were digested with 0.25% trypsin (containing EDTA) to obtain a single cell suspension, and the cells were divided into 1×10 6 The density of cells / well was seeded in a 12-well cell culture plate with cell slides placed, at 37°C, 5% CO 2 Cultured in the incubator for 2 days.

[0060] After the bacterial strain Lactobacillus reuteri 1723 of the present invention, the commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) are activated by the second generation, take the bacterial liquid at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard Clear, obtain bacteria sludge, resuspend in DMEM complete medium (without double antibody) containing 10% fetal bovine serum, take ...

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Abstract

The invention relates to the field of microorganisms and discloses lactobacillus reuteri with a weight reducing function and an application of the lactobacillus reuteri. The lactobacillus reuteri 1723strain is separated from butter collected from countries in Xigaze City, Tibet Autonomous Region, China. The strain is preserved in CGMCC (China General Microbiological Culture Collection Center) inOctober 23, 2019, and the microbial preservation number is CGMCC NO.18729. The lactobacillus reuteri 1723 has good tolerance and adhesiveness, can remarkably reduce body weight of rats, reduces fat accumulation in body, reduces body fat ratio, has good fermentation characteristics, can be applied to fermented milk, and can also be applied to drugs, health care products or healthy food for preventing and treating obesity.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a strain of Lactobacillus reuteri with weight loss function and its application. Background technique [0002] In recent years, with the development of social economy and the continuous improvement of people's living standards, the prevalence of overweight and obesity among Chinese residents has shown a rapid growth trend. According to reports, the average obesity rate in China reached 12% in 2018, and the total number of obese people ranks first in the world. At the same time, the characteristics of the obese population are showing a younger trend, and this proportion may continue to rise in the future. According to the results of the Chinese Resident Health and Nutrition Survey, from 1997 to 2009, the adult overweight and obesity rate increased from 25.1% to 39.6%. In 2012, the overweight rate of adults aged 18 and over in my country reached 30.1%, the obesity rate reached 11.9%, and t...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P3/04A23L33/135A23C9/123C12R1/225
CPCC12N1/20A61K35/747A61P3/04A23L33/135A23C9/1234A23V2002/00C12R2001/225C12N1/205A23V2400/173A23V2200/332
Inventor 俞赟霞孙盛陈苏陈丽娥李言郡陈彩玲朱珺陈作国翁璐溦周晴晴
Owner HANGZHOU WAHAHA TECH
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