Composition for inhibiting activity of 5alpha-reductase and application of composition
A composition and reductase technology, applied in the field of medicine, can solve the problems of high cost, large side effects of drugs, long cycle, etc., and achieve the effect of inhibiting 5α-reductase activity, reducing DHT content, and preventing synergistic effects
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Embodiment 1
[0041] The in vitro study of the antiproliferative activity of embodiment 1 pharmaceutical composition
[0042] 1. Purpose of the experiment
[0043] In this experiment, the MTT method was used to measure the inhibitory effect of the drug on the abnormal proliferation of prostate epithelial and stromal cells, and the anti-proliferation activity of the drug on the prostate hyperplasia cells was preliminarily verified by in vitro experiments.
[0044] 2. Experimental materials and methods
[0045] (1) Experimental materials
[0046] Proliferating human prostate stromal cells (WPMY-1) and proliferating human prostate epithelial cells (BPH-1) were used as experimental cells.
[0047] (2) Experimental grouping
[0048] The cell experiment is divided into a blank control group and a drug group, wherein the drug group includes a lycopene group (L), a curcumin group (C), an aromatic curcumone group (O), and a composition group (L / C / O), The dose concentrations used in each group we...
Embodiment 2
[0062] Example 2 Study on the proliferative activity of the pharmaceutical composition on papillary dermal cells
[0063] 1. Purpose of the experiment
[0064] Papillary dermal cells are specially differentiated mesenchymal cells that play a role in traditional Chinese medicine in the hair growth cycle. Stimulation and proliferation of these cells promote hair growth in the anagen phase, and such cells can be used as model cells for evaluating hair growth promoting agents.
[0065] 2. Materials and methods
[0066] Papillary dermal cells (DP cells) from human hair follicles were cultured in DMEM supplemented with 10% fetal bovine serum and 10 μl / ml streptomycin / penicillin; the culture was maintained at 37°C in an incubator with 5% CO2 In 2-3 days, the medium was changed; then the DP cells were inoculated on a 96-well plate, and after culturing for 24 hours, according to the drug concentrations of different groups (drug concentrations were the same as in Example 1), the drugs...
Embodiment 3
[0069] Example 3 Study on the Proliferation Inhibitory Activity of Pharmaceutical Compositions on Human Sebocytes
[0070] 1. Purpose of the experiment
[0071] The pilosebaceous unit constitutes a skin structure that is more sensitive to the action of androgens. In particular, sebocytes have significant 5α-reductase activity, so this cell is used as a target cell and model for androgen-dependent skin diseases such as acne.
[0072] 2. Materials and methods
[0073] Human immortalized sebocytes (SZ95 sebocyte line) were cultivated in DMEM medium supplemented with 10% fetal bovine serum, streptomycin / penicillin of 10 μl / ml and recombinant human epidermal growth factor of 5 μg / mL; the culture was maintained at 37 Under ℃, 5% CO In the incubator, change culture medium every 2-3 days; Then SZ95 sebocytes are inoculated on 96 well plate, after cultivating 24h, according to the drug concentration of different groups (drug concentration is the same as embodiment 1) , drugs were ad...
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