Soil remediation agent for remedying pepper continuous cropping obstacles as well as preparation method and application thereof
A soil remediation agent and technology for continuous cropping obstacles, applied in the field of soil remediation agent for repairing pepper continuous cropping obstacles and its preparation, can solve problems such as pepper continuous cropping obstacles, achieve the effects of increasing yield and quality, improving soil compaction, and excellent repairing effect
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Embodiment 1
[0030] Trichoderma and sulfuric acid addition optimization
[0031] Set 4 recipes to optimize the addition of Trichoderma and 2M sulfuric acid: 1) 5% Trichoderma + 10% sulfuric acid; 2) 5% Trichoderma + 15% sulfuric acid; 3) 10% Trichoderma + 10% sulfuric acid ; 4) 10% Trichoderma species + 15% sulfuric acid. When raising pepper seedlings, add 20% soil remediation agent to the seedling substrate, and evaluate the growth-promoting effect on pepper seedlings. The results of seedling cultivation showed that the soil remediation agent produced by the formula of treatment 4 (10% Trichoderma + 15% sulfuric acid) had the best growth-promoting effect on pepper ( figure 1 ). The content of Trichoderma in the restorative products produced by all formulas can reach 5×10 7 CFU / g or more, and the content of Trichoderma in the soil remediation agent for continuous cropping obstacles produced by formula 4 is the highest, reaching 8×10 8 CFU / g( figure 2 ).
Embodiment 2
[0033] Optimization of conditions for sporulation by fermentation of Trichoderma
[0034] Fully mix the crushed fresh straw and mushroom slag at a ratio of 1:2, then add 15% (V / W) 2M sulfuric acid solution, mix well and stack for 1 day to stabilize the pH of the mixture at 2-2.5 , then add 10% Trichoderma liquid strains, fully mix and ferment, set 3 treatments: 1) 7 days of dark fermentation; 2) 7 days of light fermentation; 3) 7 days of daily light fermentation; 4) avoid The light was left to ferment for 3 days, and then changed to daily light to continue to stand and ferment for 4 days. Because dark fermentation is conducive to the growth of Trichoderma mycelium, light can stimulate Trichoderma sporulation, in order to improve the output of final Trichoderma spores, Trichoderma needs to carry out mycelium growth in the early stage, and then carry out concentrated sporulation in the later stage, so process 4 Trichoderma spore yield was the highest, reaching 8×10 8 CFU / g ( ...
Embodiment 3
[0036] Add 15% (V / W) of 2 moles of sulfuric acid to the mixture of crushed fresh straw and mushroom slag, mix thoroughly and stack for 1 day to stabilize the pH of the mixture at 2.5, then add 10% Trichoderma liquid Bacteria, fully mixed and then kept in the dark for 3 days, then changed to daily light and continued to stand for 4 days, then added 2M sulfuric acid solution to adjust the pH of the material to 6.0, and then added 5% Bacillus powder, After 2 days of static fermentation, a soil remediation agent for improving pepper continuous cropping obstacles was obtained. The number of Trichoderma species in soil remediation agent ≥ 5×10 8 CFU / g, the number of Bacillus species ≥2×10 8 CFU / g, pH 7.2.
[0037] A field experiment was carried out in a pepper greenhouse for 9 years of continuous cropping in Huai'an, Jiangsu
[0038] Experiments were carried out in greenhouses with high incidence of pepper soil-borne diseases, and the incidence rate of previous pepper crops was a...
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