Construction method and application of medicine Terminalia fruit and Terminalia chebula Retz.var.tomentella Kurt. medicinal material specific chromatogram

A technology of characteristic map and construction method, applied in the field of construction of characteristic map of Myrobalan and Myrobalan tomentosa medicinal materials

Active Publication Date: 2020-06-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The two basic medicinal materials of Myrobalan (collectively referred to as) are not distinguished in clinical application at present, and there is still a lack of research on whether there is a difference in the quality of the two. Comparative Study on the Quality of Myrobalan and Myrobalan

Method used

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  • Construction method and application of medicine Terminalia fruit and Terminalia chebula Retz.var.tomentella Kurt. medicinal material specific chromatogram
  • Construction method and application of medicine Terminalia fruit and Terminalia chebula Retz.var.tomentella Kurt. medicinal material specific chromatogram
  • Construction method and application of medicine Terminalia fruit and Terminalia chebula Retz.var.tomentella Kurt. medicinal material specific chromatogram

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Experimental program
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Effect test

Embodiment 1

[0101] 1 Instruments and reagents

[0102] 1.1 Instruments Waters H-Class ultra-high performance liquid chromatography (Waters Co., Ltd., USA), WatersCORTECS T3 chromatographic column (2.1mm×150mm, 1.6μm), KQ-500DE ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd. ), ME204E one-millionth balance, XP26 one-millionth balance (Mettler-Toledo), Milli-QDirect ultrapure water system (Merck Co., Ltd.).

[0103] 1.2 Test drug 30 batches of myrobalan (collectively referred to as) medicinal materials were identified by Wei Mei, the chief pharmacist of Guangdong Yifang Pharmaceutical Co., Ltd., as the dried and mature fruit of Junziaceae plants Terminalia chebu1a Retz. and Terminalia chebula Retz.var.tomentella Kurt. . See Table 1 for sample numbers and details.

[0104] Gallic acid (batch number: 110831-201805, content: 90.8%); Corilagin (batch number: 29111623-200301, content: 100%), provided by China Food and Drug Control Institute; -5, content: 97%) is Shanghai Shidande ...

Embodiment 2

[0139] The present embodiment provides a kind of detection method of chebula (collectively referred to as) medical material, and the steps are as follows:

[0140] Preparation of the sample solution to be tested Take about 0.25 g of the chebula to be tested (generally referred to as) medicinal material powder (passed through a No. 3 sieve), accurately weighed, put in a stoppered Erlenmeyer flask, accurately add 25 ml of 70% methanol, weigh, and ultrasonically Treat (power 250W, frequency 40kHz) for 30 minutes, let cool, weigh again, make up the lost weight with 70% methanol, shake well, filter, and take the filtrate to obtain the final product.

[0141] Determination According to the chromatographic conditions in item 2.1 of Example 1, the samples were injected into an ultra-high performance liquid chromatograph.

[0142] The result is as Figure 5 As shown, compared with the characteristic maps of the medicinal material Myrobalan and the medicinal material Myrobalan versicol...

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Abstract

The invention relates to a construction method and application of a medicine Terminalia fruit and Terminalia chebula Retz.var.tomentella Kurt. medicinal material characteristic spectrum. The construction method of the medicine Terminalia fruit medicinal material specific chromatogram comprises the following steps: preparing a reference substance solution by taking gallic acid, ethyl gallate, corilagin, Chebulagic acid, myrobalan acid and chebulic acid as reference substances; dissolving a medicine Terminalia fruit in a solvent, performing ultrasonic extraction, filtering an extracting solution, and taking subsequent filtrate to obtain a medicine Terminalia fruit test solution; respectively carrying out ultra-high performance liquid chromatography detection on the reference substance solution and the medicine Terminalia fruit test solution. Chromatographic conditions of the ultra-high performance liquid chromatography comprise that a mobile phase A is acetonitrile, a mobile phase B is aphosphoric acid aqueous solution with a volume fraction of 0.1-0.3%, and an elution mode is gradient elution. The invention provides a comprehensive, effective and rapid method for controlling and evaluating the quality of the medicine terminalia fruit (collectively referred to as myrobalan).

Description

technical field [0001] The invention relates to the technical field of drug analysis and drug quality control, in particular to a construction method and application of a characteristic map of myrobalan and myrobalan tomentosa medicinal materials. Background technique [0002] Myrobalan (collectively referred to as) is a commonly used medicine of traditional Chinese medicine, and also a commonly used medicine of ethnic medicines such as Tibetan medicine and Mongolian medicine. According to the first part of the 2015 edition of "Chinese Pharmacopoeia", Myrobalan (collectively referred to as) is the Junziceae plant Terminalia chebula Retz .Or the dry ripe fruit of Terminalia chebula Retz.var.tomentella Kurt. Myrobalan (collectively) is bitter, sour, puckery in nature, flat, returns lung, large intestine meridian. It has the effects of astringing the intestines to relieve diarrhea, astringing the lungs to relieve cough, reducing fire and pharynx, and is clinically used for chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8679G01N30/8686
Inventor 李国卫吴文平邓淙友索彩仙邱韵静何嘉莹程学仁魏梅孙冬梅陈向东
Owner GUANGDONG YIFANG PHARMA
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