Extraction and purification method of active polysaccharide of Dendrobium huoshanense C.Z.Tang et S.J.Cheng

Abstract

The invention discloses an extraction and purification method of active polysaccharide of Dendrobium huoshanense C.Z.Tang et S.J.Cheng. The method comprises: a step of treating raw materials, namely astep of cleaning fresh dendrobium, and slicing to obtain dendrobium slices with the thickness of 0.2-0.4 mm; a soaking step, namely a step of putting the dendrobium slices into a container, and adding distilled water into the container until the distilled water is 0.8-1.2 cm higher than the tops of the dendrobium slices; a dissolving step, namely a step of carrying out water bath heating on the container, firstly heating for 20-30 minutes at 90-100 DEG C, and then carrying out heat preservation soaking at 25-40 DEG C to obtain a dendrobium extract solution; a concentrating step, namely a stepof concentrating the extract solution under the conditions that the vacuum degree is 0.05 to 0.07 MPa and the temperature is 20 to 25 DEG C until the relative density is 1.20 to 1.45 to obtain a concentrate; an extraction step, namely a step of adding ethanol into the concentrate, stirring for 10-15 minutes, and standing for 6-8 hours at the constant temperature of 10-25 DEG C, so as to obtain aprecipitate; a filtering step, namely a step of adding distilled water, having temperature of 50 DEG C, which is 30-60 times of the precipitate into the precipitate for dissolving, and filtering in avacuum filtering manner to obtain a filtrate; and a drying step, namely a step of heating the filtrate to 70-80 DEG C, evaporating for 50-10 minutes, concentrating under the condition that the vacuumdegree is 0.04-0.05 MPa, and drying to obtain a polysaccharide extract of Dendrobium huoshanense C.Z.Tang et S.J.Cheng.

Description

technical field [0001] The invention belongs to the technical field of medicine extraction, and specifically relates to an extraction and purification method of active polysaccharides of Dendrobium huoshanense. Background technique [0002] 1. Medicinal value of medicinal dendrobium and its polysaccharides [0003] The wild Huoshan Dendrobium, known internationally as the "Giant Panda in the Medicine World", also known as Mi Hu, has a history of more than 200 years. Originally produced in Huoshan, Anhui, my country and other places, it is the first protected variety among more than 200 endangered and scarce varieties in the national major modern Chinese medicine project. It is the best product among medicinal Dendrobium, and it is also a rare and precious Chinese herbal medicine unique to Huoshan County with geographical indications of origin. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have records. The polysaccharide contained in Dendrobium Huos...

AI Technical Summary

Problems solved by technology

In the polysaccharide extraction process of the traditional water extraction and alcohol precipitation method, the decomposition of insoluble polysaccharides such as cellulose and hemicellulose basically does not occur, so the extracted product does not contain monosaccharides and oligosaccharides produced by the decomposition of cellulose and hemicellulose chain, the above detection method is suitable; while the enzyme technology decomposes cellulose and hemicellulose during the extraction process to produce an uncertain amount of reducing monosaccharides and oligosaccharides, the above detection method is actually used to determine the total sugar in the extract content, the total sugar content is regarded as the polysaccharide content, which actually amplifies the polysaccharide content. Therefore, any patent that uses enzyme technology to extract polysaccharides from Dendrobium dendrobium claims to increase the content of polysaccharides when using the above detection method (or without explicitly mentioning the detection method) Yes, it's all unbelievable