Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of amomum maximum extract in preparation of alpha-glucosidase inhibitor drug

A technology of glucosidase and cardamom, which is applied in the field of medicinal chemistry to improve the occurrence and development, reduce production and absorption, and protect the function of the pancreas

Active Publication Date: 2020-06-19
广东省科学院南繁种业研究所
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the effect of some specific components in the extract of cardamom japonica on α-glucosidase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of amomum maximum extract in preparation of alpha-glucosidase inhibitor drug
  • Application of amomum maximum extract in preparation of alpha-glucosidase inhibitor drug
  • Application of amomum maximum extract in preparation of alpha-glucosidase inhibitor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation and identification of embodiment 1 formula I compound

[0032] Take 2 kg of dry cardamom rhizomes and crush them, heat and reflux them with 5L of 95% ethanol for three times, combine the three extracts and concentrate under reduced pressure until there is no alcohol smell, add pure water until the total volume is 1L, and use an equal volume of ethyl acetate The ester was extracted three times, and the combined ethyl acetate extracts were concentrated to dryness under reduced pressure.

[0033] The ethyl acetate extract was subjected to silica gel column chromatography with petroleum ether-acetone gradient elution. Collect petroleum ether-acetone 95:5 eluted fractions and recover to dryness under reduced pressure. After silica gel column chromatography, dichloromethane-methanol gradient elution, collect 99:1 elution fraction, this fraction utilizes Sephadex LH-20 gel column chromatography, dichloromethane-methanol 1:1 elution, obtain formula I compound.

Embodiment 2

[0034] Preparation and identification of embodiment 2 formula I compound

[0035] Take 2 kilograms of dried cardamom rhizomes and grind them, extract by percolation with 20L 95% ethanol, combine the alcohol extracts, concentrate under reduced pressure to about 2L, add 500 grams of pretreated macroporous resin (resin model can be D101, AB -8, HPD-100 and HP20, etc.). After fully volatilizing to remove ethanol, pack the column, ethanol-water gradient elution (0, 30%, 60%, 80% and 95% ethanol), 80% ethanol eluted fraction is the target fraction, and concentrate to dryness under reduced pressure. Through silica gel column chromatography, petroleum ether-acetone gradient elution. Collect petroleum ether-acetone 95:5 eluted fractions and recover to dryness under reduced pressure. After silica gel column chromatography, dichloromethane-methanol gradient elution, collect 99:1 elution fraction, this fraction utilizes Sephadex LH-20 gel column chromatography, dichloromethane-methanol ...

Embodiment 3

[0036] Preparation and identification of embodiment 3 formula I compound

[0037]Take 2 kilograms of dried cardamom rhizomes and grind them, heat and reflux them with 5L of methanol to extract three times, combine the three extracts and combine the alcohol extracts, concentrate under reduced pressure to about 2L, add 500 grams of pretreated macroporous resin (resin type can be for D101, AB-8, HPD-100 and HP20, etc.). After fully volatilizing to remove methanol, pack the column, ethanol-water gradient elution (0, 30%, 60%, 80% and 95% ethanol), the fraction eluted with 80% ethanol is the target fraction, and concentrate to dryness under reduced pressure. Through silica gel column chromatography, petroleum ether-acetone gradient elution. Collect petroleum ether-acetone 95:5 eluted fractions, and recover to dryness under reduced pressure. After silica gel column chromatography, dichloromethane-methanol gradient elution, collect 99:1 elution fraction, this fraction utilizes Seph...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of medical chemistry, in particular to application of amomum maximum extract in preparation of alpha-glucosidase inhibitor drugs. Specifically, the invention relatesto application of a compound shown in a formula I or pharmaceutically acceptable salt thereof in preparation of alpha-glucosidase inhibitor drugs. The compound shown in the formula I is extracted andseparated from complicated chemical components of amomum maximum, and can remarkably inhibit the activity of alpha-glucosidase.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to the application of cardamom japonicus extract in the preparation of α-glucosidase inhibitor drugs. Background technique [0002] α-glucosidase (α-glucosidase) mainly includes enzymes such as maltase, sucrase, isomerized maltase, and lactase, which are widely distributed in the brush border of the small intestinal villous mucosa cells and participate in the digestion of food, glycoprotein It is a general term for a class of enzymes that can catalyze the hydrolysis of α-glucose groups from the non-reducing ends of substrates containing α-glucosidic bonds. [0003] α-glucosidase affects the formation of complex carbohydrates on the cell surface, and diseases such as diabetes, obesity, hyperlipidemia, inflammation, cancer, immune response, and viral infection are closely related to the formation of complex carbohydrates on the cell surface, so α-glucosidase inhibitors not only hav...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/341A61P3/10A61P3/04A61P3/06A61P29/00A61P35/00A61P37/02A61P31/12A61P31/18A61P35/02C07D307/60
CPCA61K31/341A61P3/04A61P3/06A61P3/10A61P29/00A61P31/12A61P31/18A61P35/00A61P35/02A61P37/02C07D307/60
Inventor 卢传礼刘娟李亚军
Owner 广东省科学院南繁种业研究所