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Method for increasing yield of methane by using anaerobic granular sludge loaded with biological nano Fe3O4

An anaerobic granular sludge and bio-nano technology, applied in the field of anaerobic biological treatment, can solve the problems of high price, complicated preparation process, and the role of microbial electron transfer process in difficult particles, so as to improve efficiency, simple operation, and promote Effects of direct interspecies electron transfer

Active Publication Date: 2020-06-19
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these studies all use artificially synthesized chemical magnetite, which requires a strict preparation environment such as high temperature, and the preparation process is complicated and expensive.
At the same time, for the anaerobic granular sludge system, the direct addition of chemical magnetite can only make it distributed outside the aggregated particles, and it is difficult to play a role in the electron transfer process of microorganisms inside the particles.

Method used

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  • Method for increasing yield of methane by using anaerobic granular sludge loaded with biological nano Fe3O4
  • Method for increasing yield of methane by using anaerobic granular sludge loaded with biological nano Fe3O4
  • Method for increasing yield of methane by using anaerobic granular sludge loaded with biological nano Fe3O4

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The composition of the trace element solution is as follows: NiSO 4 ·6H 2 0=500mg / L, MnCl 2 ·4H 2 0=500mg / L, FeSO 4 ·7H 2 0=500mg / L, ZnSO 4 ·7H 2 0=100mg / L, H 3 BO 3 =100mg / L, Na 2 MoO 4 ·2H 2 0=50mg / L, CoCl 2 ·6H 2 0=50mg / L, CuSO 4 ·5H 2 0=5mg / L.

[0029] The composition of the inorganic salt culture solution is as follows: NH 4 HCO 3 =460mg / L, KH 2 PO 4 =70mg / L, NaHCO 3 =1000mg / L, CaCl 2 =7.5mg / L, trace element solution is 1mL / L.

[0030] 1) Take the anaerobic granular sludge from the sewage treatment plant, wash it with the inorganic salt culture solution prepared above, and then place it in the headspace bottle to add the inorganic salt culture solution, and then use glucose as the carbon source to add it to the inorganic salt culture solution , To obtain the anaerobic granular sludge mixture, so that the glucose concentration in the anaerobic granular sludge mixture is 2500mg / L, and the sludge concentration is 4g MLVSS / L.

[0031] 2) Prepare FeCl with a concentration of 0...

Embodiment 2

[0038] 1) Take the anaerobic granular sludge from the sewage treatment plant and wash it with the inorganic salt culture solution prepared in Example 1, then place it in a headspace bottle to add the inorganic salt culture solution, and then use glucose as the carbon source to add it to the inorganic salt culture In the liquid, an anaerobic granular sludge mixture is obtained, so that the glucose concentration in the anaerobic granular sludge mixture is 2500 mg / L and the sludge concentration is 4 g MLVSS / L.

[0039] 2) Prepare FeCl with a concentration of 0.4mol / L 3 ·6H 2 O solution and NaOH solution with a concentration of 10mol / L, in FeCl 3 ·6H 2 The NaOH solution was slowly added to the O solution while stirring to pH=7, and the obtained precipitate was washed with water to obtain an amorphous iron oxyhydroxide suspension.

[0040] Add amorphous iron oxyhydroxide suspension and sodium acetate as an electron donor to the anaerobic granular sludge mixture to make the iron oxyhydrox...

Embodiment 3

[0046] 1) Take the anaerobic granular sludge from the sewage treatment plant and wash it with the inorganic salt culture solution prepared in Example 1, then place it in a headspace bottle to add the inorganic salt culture solution, and then use glucose as the carbon source to add it to the inorganic salt culture In the liquid, an anaerobic granular sludge mixture is obtained, so that the glucose concentration in the anaerobic granular sludge mixture is 2500 mg / L and the sludge concentration is 4 g MLVSS / L.

[0047] 2) Prepare FeCl with a concentration of 0.4mol / L 3 ·6H 2 O solution and NaOH solution with a concentration of 10mol / L, in FeCl 3 ·6H 2 The NaOH solution was slowly added to the O solution while stirring to pH=7, and the obtained precipitate was washed with water to obtain an amorphous iron oxyhydroxide suspension.

[0048] Add amorphous iron oxyhydroxide suspension and sodium pyruvate as an electron donor to the anaerobic granular sludge mixture to make the concentration...

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Abstract

The invention discloses a method for increasing methane yield by anaerobic granular sludge loaded with biological nano Fe3O4, and belongs to the technical field of anaerobic biological treatment. Themethod comprises the following steps: adding an inorganic salt culture solution and a carbon source into anaerobic granular sludge to prepare an anaerobic granular sludge mixed solution; adding amorphous iron oxyhydroxide suspension and an electron donor into the reaction system, and controlling the pH value of the reaction system; and carrying out a reaction to obtain anaerobic granular sludge loaded with biological nano Fe3O4. The anaerobic granular sludge loaded with biological nano Fe3O4 is added into an anaerobic reactor, and organic wastewater is taken as inlet water to react to determine the yield of methane. According to the method, microorganisms in the anaerobic granular sludge are used for dissimilating and reducing Fe (III) into biological Fe3O4 nanoparticles, in-situ loading of the Fe3O4 nanoparticles in the anaerobic granular sludge is achieved, direct interspecific electron transfer of the microorganisms is enhanced through the Fe3O4 nanoparticles, and the methane production efficiency of decomposing organic matter by the anaerobic granular sludge is improved.

Description

Technical field [0001] The invention belongs to the technical field of anaerobic biological treatment, and in particular relates to a loaded biological nano Fe 3 O 4 Of anaerobic granular sludge to increase methane production. Background technique [0002] Anaerobic biological treatment technology is a sewage treatment technology that uses anaerobic and facultative anaerobic microorganisms to convert complex organic substrates in sewage into inorganic substances. The technology has the characteristics of high organic load, low energy consumption, and the ability to generate renewable energy-biogas (methane and carbon dioxide). Anaerobic granular sludge is a special kind of microbial aggregate formed by the aggregation of anaerobic / facultative microorganisms with different functions. Because of its high metabolic activity, high biological density, and good sedimentation performance, it has become an efficient anaerobic Oxygen biological treatment technology. Anaerobic granular s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28
CPCC02F3/28C02F3/2806C02F2305/06Y02E50/30
Inventor 全向春郑宇
Owner BEIJING NORMAL UNIVERSITY
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