A loaded bionano fe 3 o 4 Anaerobic Granular Sludge as a Method for Improving Methane Production

An anaerobic granular sludge and bio-nano technology, which is applied in the field of anaerobic biological treatment, can solve the problems of difficult particle internal microbial electron transfer process, complex preparation process, high price, etc., to achieve the promotion of direct interspecies electron transfer, Ease of operation and improved efficiency

Active Publication Date: 2021-03-16
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these studies all use artificially synthesized chemical magnetite, which requires a strict preparation environment such as high temperature, and the preparation process is complicated and expensive.
At the same time, for the anaerobic granular sludge system, the direct addition of chemical magnetite can only make it distributed outside the aggregated particles, and it is difficult to play a role in the electron transfer process of microorganisms inside the particles.

Method used

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  • A loaded bionano fe <sub>3</sub> o <sub>4</sub> Anaerobic Granular Sludge as a Method for Improving Methane Production
  • A loaded bionano fe <sub>3</sub> o <sub>4</sub> Anaerobic Granular Sludge as a Method for Improving Methane Production
  • A loaded bionano fe <sub>3</sub> o <sub>4</sub> Anaerobic Granular Sludge as a Method for Improving Methane Production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]The composition of the formulation of trace elements is as follows: NISO4· 6h20 = 500mg / L, MNCL2· 4h20 = 500mg / L, FESO4· 7h20 = 500mg / L, ZNSO4· 7h20 = 100mg / L, H3BO3= 100mg / L, NA2MOO4· 2h20 = 50mg / L, CoCl2· 6h20 = 50mg / L, CUSO4· 5h20 = 5 mg / L.

[0029]The composition for formulated inorganic salt culture is as follows: NH4HCO3= 460mg / l, kh2PO4= 70mg / L, NAHCO3= 1000mg / L, CACL2= 7.5 mg / L, the microelectric solution is 1 mL / L.

[0030]1) Take the anaerobic particle sludge of the sewage treatment plant, cleaned with the above-formulated inorganic salt culture fluid, and then placed in the top and space bottle, then add inorganic salt culture solution, then adding glucose to the inorganic salt culture solution. The anaerobic particle sludge mixture was obtained, allowing the concentration of glucose concentration in anaerobic particulate sludge mixture to 2500 mg / L, so that the sludge concentration was 4 g mlVSS / L.

[0031]2) FECL for formulation concentration ...

Embodiment 2

[0038]1) Take the anaerobic particle sludge of the sewage treatment plant, cleaned with the inorganic salt culture fluid formulated by Example 1, and then placed in the top and spaced bottle, then adding the inorganic salt culture, and added to the inorganic salt culture. In the liquid, anaerobic particulate sludge mixture was obtained, and the concentration of the glucose concentration in anaerobic particulate sludge mixture was 2500 mg / L, so that the sludge concentration was 4 g mlVSS / L.

[0039]2) FECL for formulation concentration is 0.4mol / L3· 6h2O solution and NaOH solution having a concentration of 10 mol / L, in FeCl3· 6h2The NaOH solution was slowly added while stirring while stirring, and the resulting precipitate was washed to obtain an amorphous hydroxyl oxide suspension obtained by PH = 7.

[0040]An amorphous hydroxylide iron hydrogen peroxide suspension is added to the anaerobic particulate sludge mixture and the sodium acetate as the electron donate, and the concentr...

Embodiment 3

[0046]1) Take the anaerobic particle sludge of the sewage treatment plant, cleaned with the inorganic salt culture fluid formulated by Example 1, and then placed in the top and spaced bottle, then adding the inorganic salt culture, and added to the inorganic salt culture. In the liquid, anaerobic particulate sludge mixture was obtained, and the concentration of the glucose concentration in anaerobic particulate sludge mixture was 2500 mg / L, so that the sludge concentration was 4 g mlVSS / L.

[0047]2) FECL for formulation concentration is 0.4mol / L3· 6h2O solution and NaOH solution having a concentration of 10 mol / L, in FeCl3· 6h2The NaOH solution was slowly added while stirring while stirring, and the resulting precipitate was washed to obtain an amorphous hydroxyl oxide suspension obtained by PH = 7.

[0048]An amorphous hydroxylide iron oxide suspension and a pyruvate of the electron donor were added to the anaerobic particulate sludge mixture, and the concentration of the hydrox...

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Abstract

The invention discloses a method for increasing methane yield by anaerobic granular sludge loaded with biological nano Fe3O4, and belongs to the technical field of anaerobic biological treatment. Themethod comprises the following steps: adding an inorganic salt culture solution and a carbon source into anaerobic granular sludge to prepare an anaerobic granular sludge mixed solution; adding amorphous iron oxyhydroxide suspension and an electron donor into the reaction system, and controlling the pH value of the reaction system; and carrying out a reaction to obtain anaerobic granular sludge loaded with biological nano Fe3O4. The anaerobic granular sludge loaded with biological nano Fe3O4 is added into an anaerobic reactor, and organic wastewater is taken as inlet water to react to determine the yield of methane. According to the method, microorganisms in the anaerobic granular sludge are used for dissimilating and reducing Fe (III) into biological Fe3O4 nanoparticles, in-situ loading of the Fe3O4 nanoparticles in the anaerobic granular sludge is achieved, direct interspecific electron transfer of the microorganisms is enhanced through the Fe3O4 nanoparticles, and the methane production efficiency of decomposing organic matter by the anaerobic granular sludge is improved.

Description

Technical field[0001]The present invention belongs to the field of anaerobic biological treatment, in particular, to a load biological nanometers.3O4Aerobic particulate sludge to increase methane production.Background technique[0002]Anaerobic biological treatment techniques are a sewage treatment technique for transforming complex organic matter substrates in sewage with anaerobic and polyaerobic microorganisms into inorganic substances. The technique has the characteristics of high organic load, small energy consumption, and can produce renewable energy-biogas (methane and carbon dioxide). Anaerobic particle sludge is a special microbial aggregate formed by different functions of anaerobic / polymultry microbial aggregation, which has become an efficient hate that it has the advantages of high metabolic activity, high biological density and good segmentation performance. Oxygen treatment technology. Anaerobic particle sludge is constructed of hydrolyzed fermenting bacteria, hydroge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28
CPCC02F3/28C02F3/2806C02F2305/06Y02E50/30
Inventor 全向春郑宇
Owner BEIJING NORMAL UNIVERSITY
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