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Method for extracting anthocyanin from fresh tea leaves

A technology for anthocyanin and fresh tea leaves is applied in the field of natural medicine extraction, which can solve the problems of low purity and low extraction efficiency, and achieve the effects of high extraction efficiency, good reproducibility, and large-scale industrial application.

Active Publication Date: 2020-06-19
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above prior art, the present invention provides a method for extracting anthocyanins from fresh tea leaves, so as to solve the problems of low extraction efficiency and low purity when using the prior art to extract tea anthocyanins

Method used

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  • Method for extracting anthocyanin from fresh tea leaves
  • Method for extracting anthocyanin from fresh tea leaves
  • Method for extracting anthocyanin from fresh tea leaves

Examples

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Effect test

Embodiment 1

[0041] A method for extracting anthocyanins from fresh tea leaves, comprising the following steps:

[0042] S1: Accurately weigh 5g of "Ziyan" tea tree fresh leaves and add them to a pre-cooled mortar, then add liquid nitrogen for quick grinding; then transfer the ground tea leaves to a beaker, and add 40mL of them to a concentration of 0.4g / mL citric acid solution, after mixing to obtain a slurry;

[0043]S2: Weigh 1g of cellulase, 0.5g of pectinase and 0.3g of tannase, and mix them evenly to obtain mixed enzyme; weigh 180mg of mixed enzyme, and add it to the slurry, stir the slurry evenly Transfer to an ultrasonic reactor, perform ultrasonic enzymolysis at 37°C for 60 minutes, and control the ultrasonic frequency at about 45 kHz; then filter with cation exchange resin coated with montmorillonite, and collect the filtrate;

[0044] S3: Mix the filtrate with 1% HCl-MeOH solution at a volume ratio of 1:1.5, extract at 0°C for 2 hours, then centrifuge to collect the supernatant...

Embodiment 2

[0048] A method for extracting anthocyanins from fresh tea leaves, comprising the following steps:

[0049] S1: Accurately weigh 5g of "Zijuan" tea tree fresh leaves and add them to a pre-cooled mortar, then add liquid nitrogen to quickly grind; then transfer the ground tea leaves to a beaker, and add 50mL of them to a concentration of 0.1g / mL citric acid solution, after mixing to obtain a slurry;

[0050] S2: Weigh 1g of cellulase, 0.4g of pectinase and 0.5g of tannase, and mix them evenly to obtain mixed enzyme; weigh 275mg of mixed enzyme, and add it to the slurry, stir the slurry evenly Transfer to an ultrasonic reactor, perform ultrasonic enzymolysis at 40°C for 45 minutes, and control the ultrasonic frequency at about 50 kHz; then filter with cation exchange resin coated with montmorillonite, and collect the filtrate;

[0051] S3: Mix the filtrate with 1% HCl-MeOH solution at a volume ratio of 1:1.5, extract at 2°C for 2 hours, then centrifuge to collect the supernatant...

Embodiment 3

[0055] A method for extracting anthocyanins from fresh tea leaves, comprising the following steps:

[0056] S1: Accurately weigh 5g of "Ziyan" fresh tea leaves and add them to a pre-cooled mortar, then add liquid nitrogen to quickly grind; then transfer the ground tea leaves to a beaker, and add 25mL of 0.5g / mL Citric acid solution, get slurry after mixing;

[0057] S2: Weigh 1g of cellulase, 0.4g of pectinase and 0.5g of tannase, and mix them uniformly to obtain mixed enzyme; weigh 60mg of mixed enzyme, and add it to the slurry, stir the slurry evenly Transfer to an ultrasonic reactor for enzymatic hydrolysis at 37°C for 45 minutes; then filter with a cation exchange resin coated with montmorillonite, and collect the filtrate;

[0058] S3: Mix the filtrate with 1% HCl-MeOH solution at a volume ratio of 1:1.5, extract at 0°C for 3 hours, then centrifuge to collect the supernatant; then mix the resulting supernatant with 1% HCl-MeOH The solution was mixed at a volume ratio of...

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Abstract

The invention discloses a method for extracting anthocyanin from fresh tea leaves. The method comprises the following steps: (1) preparing fresh tea leaves into slurry; (2) carrying out enzymolysis treatment on the slurry, and collecting filtrate after enzymolysis; (3) extracting the filtrate obtained in the step (2) for at least twice to obtain an extracting solution; (4) distilling the extracting solution at the temperature of 70-75 DEG C and the pressure of 0.05-0.1 MPa to obtain an extract; and (5) carrying out adsorption and elution treatment on the extract by using a DM130 resin adsorption column, concentrating the eluent, and drying the eluent to obtain anthocyanin. By adopting the method disclosed by the invention, the anthocyanin yield is 2% or above, the product purity is 40% orabove, and the problems of low extraction efficiency and low purity when the anthocyanin of the tea leaves is extracted in the prior art can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of natural medicine extraction, and in particular relates to a method for extracting anthocyanins from fresh tea leaves. Background technique [0002] Anthocyanin, also known as anthocyanin, is a water-soluble natural pigment that widely exists in plants in nature. Anthocyanins are secondary metabolites produced by plants, which help plants resist adversity, such as freezing damage, salt damage, and low phosphorus stress. Many studies have shown that anthocyanin is also a safe, non-toxic, beneficial antioxidant and antibacterial agent for human health, and has physiological functions such as lowering blood pressure, lowering blood fat, preventing colon cancer, and anti-inflammation. Therefore, it has great application value in food, chemical industry, medicine, etc., and how to extract anthocyanins from plants has become a key issue in its development and application. [0003] At present, plants rich in an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 唐茜李伟谭晓琴谭礼强陈玮黄嘉诚范虹利杨纯婧谢文钢张利萍王鑫
Owner SICHUAN AGRI UNIV
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