Iridoid glycoside compound as well as preparation method and application thereof

A technology of iridoid glycosides and compounds, which is applied in the field of medicine and can solve problems such as toxic and side effects

Inactive Publication Date: 2020-06-19
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because many synthetic drugs have strong toxic and side effects, people pay m

Method used

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  • Iridoid glycoside compound as well as preparation method and application thereof
  • Iridoid glycoside compound as well as preparation method and application thereof
  • Iridoid glycoside compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation of embodiment 1 compound of the present invention

[0039] (1) Take the dried medicinal material of Flos Japonica, extract twice with 70% ethanol under reflux for 2 hours each time, combine the extracts, and remove the solvent under reduced pressure to obtain the total extract. The total extract was dissolved in water, separated by HP-20 macroporous adsorption resin column chromatography, eluted with water, 25% ethanol, 45% ethanol, and 90% ethanol in sequence, and each eluate was collected separately, concentrated under reduced pressure to no alcohol Taste, get water elution site, 25% ethanol elution site, 45% ethanol elution site, 90% ethanol elution site;

[0040](2) Take the 45% ethanol elution part of step (1), separate it by silica gel column chromatography, and use chloroform-methanol-water gradient elution (100:0:0; 95:5:0; 90:10:0; 85:15:4; 75:25:6to 60:40:8, v / v / v), and 16 fractions (A-P) were collected, and fraction F was subjected to SiO2 co...

Embodiment 2

[0042] The preparation of embodiment 2 compounds of the present invention

[0043] (1) Take the dried medicinal material of Flos Japonica, extract twice with 70% ethanol under reflux for 2 hours each time, combine the extracts, and remove the solvent under reduced pressure to obtain the total extract. The total extract is dissolved in water, separated by HP-20 macroporous adsorption resin column chromatography, eluted with water, 30% ethanol, 50% ethanol, and 95% ethanol in turn, and the eluents are collected respectively, concentrated under reduced pressure to no alcohol Taste, get water elution site, 30% ethanol elution site, 50% ethanol elution site, 95% ethanol elution site;

[0044] (2) Take the 50% ethanol elution part of step (1), separate through silica gel column chromatography, and use chloroform-methanol-water gradient elution (95:5:0; 90:10:0; 85:15:0; 80:20:4; 70:30:6to 60:40:8, v / v / v), and 16 fractions (A-P) were collected, and Fraction F was subjected to SiO2 c...

Embodiment 3

[0046] The preparation of embodiment 3 compounds of the present invention

[0047] (1) Take the dried medicinal material of Flos Japonica, extract twice with 70% ethanol under reflux for 2 hours each time, combine the extracts, and remove the solvent under reduced pressure to obtain the total extract. The total extract is dissolved in water, separated by HP-20 macroporous adsorption resin column chromatography, eluted with water, 35% ethanol, 55% ethanol, and 100% ethanol in turn, and the eluents are collected respectively, concentrated under reduced pressure to no alcohol Taste, get water elution site, 35% ethanol elution site, 55% ethanol elution site, 100% ethanol elution site;

[0048] (2) Take the 50% ethanol eluted part of step (1), separate it by silica gel column chromatography, and use chloroform-methanol-water gradient elution (90:10:0; 80:20:0; 75:25:0; 70:30:4to 60:40:8, v / v / v), collected 16 fractions (A-P), and fraction F was subjected to SiO2 column chromatograp...

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Abstract

The invention discloses an iridoid glycoside compound. The iridoid glycoside compound is a new chemical component discovered in lonicerae flos. A compound separated by virtue of a method is further structurally identified by virtue of physicochemical properties and modern spectroscopic measures. Activity evaluation is carried out by virtue of activity screening systems such as an LPS-induced RAW 264.7 cell inflammation model, and a result shows that the compound has a certain protecting effect on a mouse macrophage system RAW 264.7, is capable of remarkably inhibiting IL-6 and presents a relatively strong anti-inflammatory effect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a novel compound and its preparation method and application. Background technique [0002] The traditional Chinese medicine Lonicerae Flos is Lonicera macranthoides Hand.-Mazz., Lonicera hypoglauca Miq., Lonicera confusa D C., Lonicera fulvotomentosa, Lonicera fulvotomentosa, Caprifoliaceae Lonicera The dried buds or flowers with first blooms of Hsu etS.C.Cheng are important medicinal resources. Have heat-clearing and toxic substances removing, evacuate wind-heat effect, be clinically used for carbuncle furuncle, sore throat, erysipelas, pyretic toxin bloody dysentery, wind-heat cold, febrile disease heating. [0003] Anti-inflammatory drugs are the second largest class of drugs after anti-infective drugs in clinical treatment, including steroidal anti-inflammatory drugs (SAIDs) and non-steroidal anti-inflammatory drugs (NSAIDs). However, because many synthetic drugs have stro...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61P29/00
CPCC07H17/04C07H1/08A61P29/00
Inventor 肖伟李海波姚新生于洋梅玉丹王振中邓奕杨彪
Owner JIANGSU KANION PHARMA CO LTD
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