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Research method for pharmacologic action of mangiferin on mouse diabetes

A pharmacological effect, mangiferin technology, applied in the field of medicine, can solve the problems of limited reports, achieve the effect of inhibiting renal interstitial fibrosis, preventing renal interstitial fibrosis, and reducing phosphorylation

Inactive Publication Date: 2020-06-23
JILIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Although the therapeutic effect of mangiferin on DN has also been confirmed in previous studies, reports on the mechanism of action of mangiferin on renal interstitial fibrosis in DN are still limited.

Method used

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  • Research method for pharmacologic action of mangiferin on mouse diabetes
  • Research method for pharmacologic action of mangiferin on mouse diabetes
  • Research method for pharmacologic action of mangiferin on mouse diabetes

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Embodiment Construction

[0031] In the following, the invention will be further described with reference to the drawings and specific implementations:

[0032] See Figure 1-7 , The research method of mangiferin according to the embodiment of the present invention on the pharmacological effects of diabetes in mice, including material selection and experimental methods, biochemical parameter evaluation, histological analysis, measurement of reactive oxygen species, determination of malondialdehyde and antioxidant enzymes, kidney tissue Inflammatory factor analysis, immunofluorescence staining, western blotting and statistical analysis, the material selection and experimental methods are as follows:

[0033] Material selection: Take 70 7-week-old C57BL / 6 male mice weighing 21g±2g, and place the mice under SPF conditions without specific pathogens at a constant room temperature of 22-25°C, humidity 45-55%, light and dark Circulate for 12 hours, freely obtain clean food and water for training;

[0034] Experim...

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Abstract

The invention discloses a research method for the pharmacologic action of mangiferin on mouse diabetes. The research method comprises material selection and an experimental method, biochemical parameter evaluation, histological analysis, measurement of reactive oxygen species (ROS), determination of malondialdehyde (MDA) and antioxidase, analysis of renal tissue inflammatory factors, immunofluorescent staining, Western blotting and statistic analysis. According to the research method, a trichrome staining method is utilized to observe renal morphology; a kit is utilized to determine a blood biochemical index; levels of inflammatory cytokines, the antioxidase, the MDA and the ROS are determined; expression of fibronectin, collagen I and alpha-SMA is detected by an immunohistochemical method, and regulation of paths of TGF-beta 1 and phosphatase and tensin homolog / phosphatidylinositol 3-hydroxy kinase / protein kinase B (PTEN / PI3K / Akt) is detected by Western blotting; researches show thatthe mangiferin can significantly improve the renal dysfunction of diabetic mice; renal interstitial fibrosis can be prevented by reducing positive expression of fibronectin (FN), collagen type I (ColI) and alpha-smooth muscle actin (SMA) during therapy with the mangiferin; and meanwhile, mangiferin increases the antioxidase, reduces phosphorylation of the PI3K and Akt, inhibits the renal interstitial fibrosis, and provides more theoretical foundations for clinical application of traditional Chinese medicine in treating diabetes.

Description

Technical field [0001] The present invention relates to the field of medicine, in particular to a research method for the pharmacological effects of mangiferin on diabetes in mice. Background technique [0002] Diabetes is a metabolic disease characterized by chronic elevated blood sugar. Hyperglycemia is caused by impaired insulin production and insulin resistance. Long-term chronic hyperglycemia can cause fat and protein metabolism disorders, which in turn leads to a series of complications in the retina, kidneys and nervous system. Diabetic nephropathy (DN) is the most serious complication of diabetes. It is characterized by the loss of kidney cells, which are replaced by extracellular matrix (ECM), which ultimately leads to glomerular sclerosis and tubular interstitial fibrosis. [0003] Oxidative stress and chronic inflammation play a key role in the development of DN. Hyperglycemia increases the production of reactive oxygen species, which activate signal transduction and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/352A61P3/10A61P13/12G01N33/68
CPCA61K31/352A61K49/0008A61P3/10A61P13/12G01N33/6827G01N33/6887G01N2333/4712G01N2333/78
Inventor 宋艳艳高航唐克
Owner JILIN UNIV
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