Research method for pharmacologic action of mangiferin on mouse diabetes

Abstract

The invention discloses a research method for the pharmacologic action of mangiferin on mouse diabetes. The research method comprises material selection and an experimental method, biochemical parameter evaluation, histological analysis, measurement of reactive oxygen species (ROS), determination of malondialdehyde (MDA) and antioxidase, analysis of renal tissue inflammatory factors, immunofluorescent staining, Western blotting and statistic analysis. According to the research method, a trichrome staining method is utilized to observe renal morphology; a kit is utilized to determine a blood biochemical index; levels of inflammatory cytokines, the antioxidase, the MDA and the ROS are determined; expression of fibronectin, collagen I and alpha-SMA is detected by an immunohistochemical method, and regulation of paths of TGF-beta 1 and phosphatase and tensin homolog/phosphatidylinositol 3-hydroxy kinase/protein kinase B (PTEN/PI3K/Akt) is detected by Western blotting; researches show thatthe mangiferin can significantly improve the renal dysfunction of diabetic mice; renal interstitial fibrosis can be prevented by reducing positive expression of fibronectin (FN), collagen type I (ColI) and alpha-smooth muscle actin (SMA) during therapy with the mangiferin; and meanwhile, mangiferin increases the antioxidase, reduces phosphorylation of the PI3K and Akt, inhibits the renal interstitial fibrosis, and provides more theoretical foundations for clinical application of traditional Chinese medicine in treating diabetes.

Description

Technical field [0001] The present invention relates to the field of medicine, in particular to a research method for the pharmacological effects of mangiferin on diabetes in mice. Background technique [0002] Diabetes is a metabolic disease characterized by chronic elevated blood sugar. Hyperglycemia is caused by impaired insulin production and insulin resistance. Long-term chronic hyperglycemia can cause fat and protein metabolism disorders, which in turn leads to a series of complications in the retina, kidneys and nervous system. Diabetic nephropathy (DN) is the most serious complication of diabetes. It is characterized by the loss of kidney cells, which are replaced by extracellular matrix (ECM), which ultimately leads to glomerular sclerosis and tubular interstitial fibrosis. [0003] Oxidative stress and chronic inflammation play a key role in the development of DN. Hyperglycemia increases the production of reactive oxygen species, which activate signal transduction and...

AI Technical Summary

Problems solved by technology

Although the therapeutic effect of mangiferin on DN has also been confirmed in previous studies, reports on the mechanism of action of mangiferin on renal interstitial fibrosis in DN are still limited.

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