Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of collagen hydrophobic modifier and its preparation method and the method for modifying collagen

A hydrophobic modifier and collagen technology, applied in textiles and papermaking, single-component protein rayon, organic chemistry, etc., can solve the problems of easy introduction of toxicity by organic solvents, low modification efficiency, and difficulty in control, etc., to improve the structure Stability and electrostatic spinnability, effect of reducing denaturation and improving hydrophobicity

Active Publication Date: 2021-08-03
FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current collagen hydrophobic modifiers (acid anhydride, acid chloride, fatty aldehyde, etc.) have problems such as uneven solid / liquid reaction, difficulty in control, easy introduction of toxicity by organic solvents, and low modification efficiency.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of collagen hydrophobic modifier and its preparation method and the method for modifying collagen
  • A kind of collagen hydrophobic modifier and its preparation method and the method for modifying collagen
  • A kind of collagen hydrophobic modifier and its preparation method and the method for modifying collagen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0035] Dissolve 0.1 part of lauric acid in 1 part of dimethylformamide (DMF) and place in a 100 mL round bottom flask. Afterwards, weigh 0.5 part of thio-NHS and 0.5 part of dicyclohexylcarbodiimide, add them in sequence to the DMF solution of lauric acid, keep stirring under airtight conditions, and react at room temperature for 2 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 0.5 parts of ethyl acetate, let it stand for 48 hours, precipitate out, filter the filter cake, dry in air, and obtain lauric acid-thio NHS ester.

[0036] A method for modifying collagen, comprising the steps of:

[0037] Weigh 10 parts of fish skin collagen and dissolve in 5 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 0.5 mol / L NaOH solution to adjust the pH to 9-10. Add 0.01 part of lauri...

Embodiment 2

[0039] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0040] 10 parts of octanoic acid was dissolved in 20 parts of dimethylformamide (DMF) and placed in a 100 mL round bottom flask. Afterwards, 20 parts of thio-NHS and 30 parts of dicyclohexylcarbodiimide were weighed, added sequentially to the DMF solution of octanoic acid, stirred under airtight conditions, and reacted at room temperature for 2 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 50 parts of ethyl acetate, let it stand for 2 hours, precipitate out, collect the filter cake by filtration, and dry in air to obtain octanoic acid-thio NHS ester.

[0041] A method for modifying collagen, comprising the steps of:

[0042] Weigh 150 parts of porcine cartilage collagen and dissolve in 50 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 5 mol / L NaOH solution to adjust t...

Embodiment 3

[0044] A preparation method of collagen hydrophobic modifier, comprising the following steps:

[0045] 5 parts of palmitic acid were dissolved in 10 parts of dimethylformamide (DMF) and placed in a 100 mL round bottom flask. Afterwards, weigh 10 parts of thio-NHS and 15 parts of dicyclohexylcarbodiimide, add them in order to the DMF solution of palmitic acid, keep stirring under closed conditions, and react at room temperature for 13 hours. After the reaction was over, the insoluble matter was removed by filtration and the filtrate was collected. Add 25 parts of ethyl acetate, let it stand for 24 hours, precipitate out, collect the filter cake by filtration, and dry in air to obtain palmitic acid-thio NHS ester.

[0046] A method for modifying collagen, comprising the steps of:

[0047]Weigh 80 parts of cowhide collagen and dissolve in 25 parts of 0.1 mol / L acetic acid solution. After the collagen is completely dissolved, add 3 mol / L NaOH solution to adjust the pH to 9-10. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a collagen hydrophobic modifier, a preparation method thereof and a method for modifying collagen. The preparation method of the collagen hydrophobic modifier: add thio-NHS and dicyclohexylcarbodiimide to the fatty acid solution in sequence, stir and mix well, react at room temperature for 2-24 hours, filter after the reaction, collect the filtrate, and then add The organic reagent is allowed to stand for 2 to 48 hours, and the solid phase product can be collected by filtration after precipitation and dried. The collagen hydrophobic modifier prepared by the invention can effectively improve the structural stability and hydrophobicity of collagen and electrospun collagen fibers.

Description

technical field [0001] The invention belongs to the technical field of collagen modification, and in particular relates to a collagen hydrophobic modifier, a preparation method thereof and a method for modifying collagen. Background technique [0002] Electrospinning technology is currently one of the most important methods for preparing nanofibers. Electrospun fibers have the characteristics of controllable diameter, large specific surface area, and high porosity, which can meet the requirements of tissue engineering scaffolds for fiber size and spatial structure. In recent years, collagen-based electrospun nanofiber materials have attracted great interest from researchers all over the world. However, most of the current reports on collagen electrospinning involve the phenomenon of collagen denaturation, such as the loss of collagen triple helix conformation, the decrease of collagen denaturation temperature and relative molecular weight, and the dissolution of electrospun ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D207/46C08H1/00D01F4/00D01F8/02
CPCC07D207/46C08H1/00D01F4/00D01F8/02
Inventor 刘文涛李培玉白聪张元智陈胜李国英
Owner FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com