Real-time reaction thermal cycle device with air guide structure in PCR reaction container

A technology of air guide structure and reaction container, which is applied in the field of PCR reaction, can solve the problems of poor PCR reaction effect, uneven temperature of samples, and lack of air guide structure, etc., and achieves a good and effective thermal cycle system, reasonable design, and improved The effect of the reaction effect

Pending Publication Date: 2020-06-23
郑钰树
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a real-time reaction heat cycle device with an air guide structure in a PCR reaction container, so as to solve the problem that DNA fragments need to be heated or cooled during the PCR reaction in the PCR reaction container proposed in the background art , so that the DNA fragments can be lengthened or derived. The heating process in the traditional PCR reaction container is generally realized by resistance wire or electric heating module, but because the resistance wire an

Method used

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  • Real-time reaction thermal cycle device with air guide structure in PCR reaction container
  • Real-time reaction thermal cycle device with air guide structure in PCR reaction container
  • Real-time reaction thermal cycle device with air guide structure in PCR reaction container

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Embodiment 1

[0029] see Figure 1-4 , a real-time reaction heat cycle device with an air guide structure in a PCR reaction container, comprising a reactor shell 1, a top shell 3, a liquid storage chamber 19 and a reaction chamber 29, the top of the reactor shell 1 is installed with a hinge The top shell 3, the inside of the top shell 3 is fixedly equipped with a UV germicidal lamp tube 9, and the outside of the UV germicidal lamp tube 9 is fixedly installed on the outer surface of the top shell 3 with a light-transmitting plate 8, through which the light-transmitting plate is installed 8. When the UV germicidal lamp tube 9 is turned on for irradiation, it can pass through the light-transmitting plate 8 to carry out good sterilization treatment in the reaction chamber 29. The inside of the reactor shell 1 is fixedly equipped with an electric board 4, and the electric board 4 is fixed A data receiving chip 5 and a single-chip microcomputer 6 are installed, and a digital display screen 2 is i...

Embodiment 2

[0032] see Figure 1-5 , a real-time reaction heat cycle device with an air guide structure in a PCR reaction container, comprising a reactor shell 1, a top shell 3, a liquid storage chamber 19 and a reaction chamber 29, the top of the reactor shell 1 is installed with a hinge The top shell 3, the inside of the top shell 3 is fixedly equipped with a UV germicidal lamp tube 9, and the outside of the UV germicidal lamp tube 9 is fixedly installed on the outer surface of the top shell 3 with a light-transmitting plate 8, through which the light-transmitting plate is installed 8. When the UV germicidal lamp tube 9 is turned on for irradiation, it can pass through the light-transmitting plate 8 to carry out good sterilization treatment in the reaction chamber 29. The inside of the reactor shell 1 is fixedly equipped with an electric board 4, and the electric board 4 is fixed A data receiving chip 5 and a single-chip microcomputer 6 are installed, and a digital display screen 2 is i...

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Abstract

The present invention discloses a real-time reaction thermal cycle device with an air guide structure in a PCR reaction container. The device comprises a reactor outer shell, a top shell, a liquid storage cavity and a reaction chamber, the top shell is arranged at a top of the reactor shell through a hinge, UV sterilization lamp tubes are fixedly arranged inside the top shell, an electric plate isfixedly arranged inside the reactor outer shell, a data receiving chip and a single chip microcomputer are fixedly arranged on the electric plate, a digital display screen is embedded and arranged inan outer wall of the reactor outer shell, and the reaction chamber, an installation inner cavity and the liquid storage cavity are respectively arranged in the reactor outer shell from top to bottom.A vortex refrigerator is arranged inside, when the reaction chamber needs cooling, the vortex refrigerator can quickly reduce temperatures in the reaction chamber, when hot steam introduced into a heat conduction cavity cools, the steam is automatically condensed while the temperatures are reduced, condensed liquid drops can flow back into the liquid storage cavity again to be utilized for temperature-rising evaporation next time, and a good and effective thermal circulation system is formed in the reaction chamber.

Description

technical field [0001] The invention belongs to the technical field of PCR reaction, in particular to a real-time reaction heat cycle device with an air guiding structure in a PCR reaction container. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase the amount of trace DNA. , Polymerase chain reaction (PCR) has increasingly become an extremely important biotechnology, providing a lot of help for scientific research, criminal investigation and other work fields. [0003] When carrying out the PCR reaction in the PCR reaction vessel, it is necessary to heat or cool down the DNA fragments so that the DNA fragments can be lengthened or derived. The heating process in the traditional PCR reaction vessel is generally realized by a resistance wire or an electric heating modul...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/34C12M1/12C12M1/00
CPCB01L3/50851B01L2300/1805
Inventor 郑钰树
Owner 郑钰树
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