Real-time reaction thermal cycle device with air guide structure in PCR reaction container

Abstract

The present invention discloses a real-time reaction thermal cycle device with an air guide structure in a PCR reaction container. The device comprises a reactor outer shell, a top shell, a liquid storage cavity and a reaction chamber, the top shell is arranged at a top of the reactor shell through a hinge, UV sterilization lamp tubes are fixedly arranged inside the top shell, an electric plate isfixedly arranged inside the reactor outer shell, a data receiving chip and a single chip microcomputer are fixedly arranged on the electric plate, a digital display screen is embedded and arranged inan outer wall of the reactor outer shell, and the reaction chamber, an installation inner cavity and the liquid storage cavity are respectively arranged in the reactor outer shell from top to bottom.A vortex refrigerator is arranged inside, when the reaction chamber needs cooling, the vortex refrigerator can quickly reduce temperatures in the reaction chamber, when hot steam introduced into a heat conduction cavity cools, the steam is automatically condensed while the temperatures are reduced, condensed liquid drops can flow back into the liquid storage cavity again to be utilized for temperature-rising evaporation next time, and a good and effective thermal circulation system is formed in the reaction chamber.

Description

technical field [0001] The invention belongs to the technical field of PCR reaction, in particular to a real-time reaction heat cycle device with an air guiding structure in a PCR reaction container. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA replication outside the body. The biggest feature of PCR is that it can greatly increase the amount of trace DNA. , Polymerase chain reaction (PCR) has increasingly become an extremely important biotechnology, providing a lot of help for scientific research, criminal investigation and other work fields. [0003] When carrying out the PCR reaction in the PCR reaction vessel, it is necessary to heat or cool down the DNA fragments so that the DNA fragments can be lengthened or derived. The heating process in the traditional PCR reaction vessel is generally realized by a resistance wire or an electric heating modul...

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Problems solved by technology

[0004] The object of the present invention is to provide a real-time reaction heat cycle device with an air guide structure in a PCR reaction container, so as to solve the problem that DNA fragments need to be heated or cooled during the PCR reaction in the PCR reaction container proposed in the background art , so that the DNA fragments can be lengthened or derived. The heating process in the traditional PCR reaction container is generally realized by resistance wire or electric heating module, but because the resistance wire and the electric heating module cannot spread all over the inner wall of the device, it will cause the samples in the device to be affected. The temperature is not uniform, resulting in poor overall processing effect, and the traditional PCR reaction container does not have an air guide structure, some hot air cannot be introduced into the reaction chamber, making the PCR reaction effect poor. In order to improve the PCR reaction effect, and in order to To ensure that the samples in the PCR reaction container are heated more evenly

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