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Method for alleviating iron-deficiency symptom of apples

A technology for apples and iron deficiency, applied in fertilization methods, botany equipment and methods, planting substrates, etc., can solve the problems of iron deficiency and iron nutrition difficult to be obtained by plants, and achieve the promotion of accumulation, improvement of stress-resistant nutrient absorption, and improvement of The effect of absorption utilization efficiency

Inactive Publication Date: 2020-06-26
JIANGSU FOUTAR AGRI SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Phosphorus and iron will antagonize to form iron-phosphorus complexes, making it difficult for plants to obtain iron nutrition. Even under normal iron conditions, excessive application of phosphorus will cause iron deficiency

Method used

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  • Method for alleviating iron-deficiency symptom of apples
  • Method for alleviating iron-deficiency symptom of apples

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The cultivation of embodiment 1 apple material

[0020] 1. The apple material comes from Pingyi sweet tea apples. Mix the seeds and wet sand evenly, and stratify them at 4°C for 1 month, and replenish water in time when the humidity is not enough. The stratified seeds were sown in the substrate and grown for 2 months under the conditions of 16h / 8h light / dark, 25°C / 20°C day / night.

[0021] 2. Select apple seedlings with consistent growth, rinse them with clean water, transplant them into a culture medium containing only vermiculite, and grow them under 16h / 8h light / dark conditions, 25°C / 20°C day / night conditions for 1 week, The culture medium used was Hoagland's nutrient solution containing 50 μM iron.

[0022] 3. Before the experimental treatment, it was transferred to clean vermiculite and water for 7 days of starvation treatment.

Embodiment 2

[0023] Embodiment 2 carries out iron deficiency and organic acid treatment to apple

[0024] The apple seedlings of above-mentioned treatment are divided into 3 groups (-Fe+5mM KH 2 PO 4 , -Fe+5mM KH 2 PO 4 +Malic acid and -Fe+5mM KH 2 PO 4 + citric acid), the -Fe treatments used were iron-free Hoagland nutrient solution and 300 μM ferrozine (an iron chelator), and all treatments were root watering.

[0025] The experimental results showed that after 14 days of cultivation, if figure 1 Shown, -Fe+5mM KH 2 PO 4 +Malic acid and -Fe+5mMKH 2 PO 4 + Citric acid treatment significantly alleviated the yellowing of leaves caused by iron deficiency.

Embodiment 3

[0026] Embodiment 3 Determination of Iron Content in the Underground Part of the Apple Shoot

[0027] Determination of total iron content: the underground part and aerial part of apple or Arabidopsis were separated, dried in a blast drying oven at 80°C for 2 days, and then ground into powder. Add 5ml of H 2 SO 4 Treat overnight to completely carbonize the material, and then digest it in a digestion furnace at 300°C. During the digestion process, add an appropriate amount of 30% H 2 o 2 until the solution is clear. After filtration, total iron / phosphorus concentrations were determined by inductively coupled plasma spectrometer (ICP-AES; Fisons ARL Accuris, Ecublens, Switzerland). Total iron / phosphorus content = C x V / M. (C: concentration, V: filtrate volume, M: dry weight).

[0028] Determination of soluble iron content: Plant material was ground in liquid nitrogen and extracted with 10 volumes of deionized water. It was then centrifuged at 5000×g for 10 minutes, and the...

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Abstract

The invention discloses a method for alleviating the iron-deficiency symptom of apples in the technical field of fruit tree physiology. By externally applying 0.5mM malic acid or citric acid, the antagonistic effect of iron and phosphorus in the apples can be effectively alleviated, and the absorption and utilization efficiency of the iron is improved, thereby alleviating the apple leaf yellowingphenomenon; the method can be applied to fruit farming in soil with the low nutrient utilization ratio; the iron-deficiency symptom of the apples can be alleviated by adding the malic acid or the citric acid into soil with iron phosphorus nutritional imbalance; it is revealed that organic acid plays an important role in an iron-deficiency-resistant process of the apples; and malic acid and citricacid supply can facilitate accumulation of the iron in rootstocks, the absorption and utilization efficiency of the iron is improved, and particularly in fruit trees, the method can be applied to theimprovement of stress-resistant nutrient absorption of the fruit trees.

Description

technical field [0001] The invention relates to the technical field of fruit tree physiology, in particular to a method for alleviating iron deficiency symptoms of apples. Background technique [0002] Iron is an essential trace element for plants. Although iron is the fourth most abundant element in the earth's crust, in alkaline or calcareous soils with good aeration, it is often difficult to meet the needs of normal plant growth and development due to the low availability of iron. According to statistics, about 40% of the cultivated land in the world suffers from different degrees of iron deficiency stress, and iron deficiency has become one of the main factors limiting crop yield and quality in agricultural production. As one of the four major fruits in the world, apples are widely faced with iron deficiency stress, and iron deficiency will seriously affect the yield and quality of fruits. At present, in order to increase fruit yield in fruit tree cultivation, a large ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C21/00A01G31/00A01G24/15
CPCA01C21/005A01G31/00
Inventor 郝玉金由春香李媛媛王小非张帅甘信环祝娜
Owner JIANGSU FOUTAR AGRI SCI & TECH
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