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Earthworm avoidance experiment box and method for evaluating joint action toxicity of imidacloprid and carbendazim

An experimental box and imidacloprid technology, which is applied in the fields of material inspection, soil material testing, animal husbandry, etc., can solve the problem that the joint toxic effect of earthworms has not been reported, and achieve the effects of avoiding external environmental interference, accurate results, and simple structure

Inactive Publication Date: 2020-06-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the single toxic effects of imidacloprid and carbendazim on environmental organisms have been reported, the combined toxic effects on earthworms when the two coexist have not been reported

Method used

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  • Earthworm avoidance experiment box and method for evaluating joint action toxicity of imidacloprid and carbendazim
  • Earthworm avoidance experiment box and method for evaluating joint action toxicity of imidacloprid and carbendazim
  • Earthworm avoidance experiment box and method for evaluating joint action toxicity of imidacloprid and carbendazim

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment one: prepare artificial standard soil and clean soil and polluted soil

[0053] 1. Preparation of artificial standard soil

[0054] Take kaolin, peat and quartz sand, grind them into powder, and then mix them, and adjust the pH value of the mixture to 6.0±0.5 with calcium carbonate to obtain artificial standard soil. The mass percentages of each component in the prepared artificial standard soil are: 65-75% of quartz sand (sand with a particle size of 0.05-0.2mm), 15-25% of kaolin (the content of kaolinite is not less than 30%), Peat (crushed, visible plant tissue required) 9-11%, calcium carbonate (powder, analytically pure) 0.1-0.3%.

[0055] 2. Preparation of contaminated soil

[0056] Contaminated soil is the soil containing pesticides, which has certain toxicity. The polluted soil in the present invention is divided into single polluted soil and joint polluted soil; wherein, single polluted soil is soil containing a single pesticide, and joint pollute...

Embodiment 2

[0061] Example 2: Test of single pesticide on avoidance behavior of earthworms

[0062] In the present embodiment, the avoidance behavior of earthworms to single polluted soil is evaluated, and the evaluation method comprises the following steps:

[0063] S1: Assemble the test box, insert the partition 4 into the compartment 3; then fill the clean soil, imidacloprid-contaminated soil or carbendazim-contaminated soil into the left chamber 5 and right chamber 6 respectively, and then remove the compartment Plate 4, put 15 earthworms into the partition room 3, cover the escape-proof cover 11, control the temperature in the experimental box to 20±1°C, the light intensity is 400-800lux, and the light time is 16h / 8h (light / darkness) , cultivated under this condition for 2 days;

[0064] S2: After the cultivation is completed, insert the dividing plate 4, count the number of earthworms in the left chamber 5 and the right chamber 6 respectively, and calculate the avoidance rate NR ac...

Embodiment 3

[0068] Example 3: Test of combined pesticides on earthworm avoidance behavior

[0069] In the past, the method of equal toxicity and equal concentration is usually used for the combined action of pesticides, but in the actual environment, it is rare for pesticides to be mixed with equal toxicity or equal concentration. The present invention considers that the concentration ratio of the mixed pesticide in the soil environment changes at different times and in different regions, and the concentration ratio of the two pesticides in the joint polluted soil is designed as 4:1, 3:2, 1:1, 2:3 and 1:4, which can represent the concentration ratio of mixed pesticides changing with time and space, which is more in line with the actual environment. In the present embodiment, the method for evaluating the avoidance behavior of earthworms to joint polluted soil comprises the following steps:

[0070] S1: Assemble the experimental box, insert the partition 4 into the compartment 3; then fil...

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Abstract

The invention discloses an earthworm avoidance experiment box and a method for evaluating the joint action toxicity of imidacloprid and carbendazim. The experiment box comprises an inner box body andan outer box body, wherein the inner box body is made of transparent materials, the middle of the inner box body is provided with a partitioning chamber, and the partitioning chamber segments the inner space of the inner box body into a left cavity and a right cavity; the side wall of the partitioning chamber is provided with through holes for earthworms to pass, and a clapboard is movably splicedin the partitioning chamber; the inner box body is positioned in the center of the outer box body; the outer box body is provided with a box cover; and the inner wall of the outer box body is provided with an illumination device and a heating device. The experiment box in the invention can accurately simulate an earthworm growth environment and avoid external environmental disturbance, and an obtained result is more accurate. Researches on an influence of the joint action of imidacloprid and carbendazim on earthworm avoidance behaviors can provide a scientific basis for quickly diagnosing a soil combined pollution effect, and meanwhile, a theoretical foundation is laid for formulating a soil combined pollution standard.

Description

technical field [0001] The invention belongs to the technical field of soil toxicity detection, and in particular relates to an earthworm avoidance test box and a method for evaluating the toxicity of the combined action of imidacloprid and carbendazim. Background technique [0002] As an important part of the terrestrial ecosystem, earthworms play an important role in the improvement of soil physical properties and plant nutrient cycle. Its life activities can not only improve soil structure, but also benefit soil aeration, drainage and water retention. Improve soil fertility by decomposing and transforming organic matter in the soil. From an ecological point of view, earthworms are at the bottom of the terrestrial ecological food chain and can enrich most pollutants in the soil. These enriched pollutants may not cause serious harm to earthworms, but they may affect Higher organisms in the food chain. At the same time, because earthworms are directly exposed to soil pollu...

Claims

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Application Information

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IPC IPC(8): A01K67/033G01N33/24
CPCA01K67/0331G01N33/24
Inventor 王彦华王强杨桂玲李薪芳王新全
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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