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Yellow light emission fluorescent probe and preparation method thereof, method for selectively detecting iron ions and PPi and cell imaging method

A fluorescent probe and selective technology, applied in the field of yellow light-emitting fluorescent probes, which can solve the problem of low detection limit

Pending Publication Date: 2020-06-26
ANHUI NORMAL UNIV
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Problems solved by technology

[0003] In recent decades, PPi-specific fluorescent probes have been developed, and fluorescence assays have attracted much attention due to their relatively low cost, high sensitivity, easy operation, reliable methods, and low detection limits; although various types of Fluorescent chemosensors can selectively recognize PPi, but only a few fluorescent chemosensors can recognize PPi in aqueous solution

Method used

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  • Yellow light emission fluorescent probe and preparation method thereof, method for selectively detecting iron ions and PPi and cell imaging method
  • Yellow light emission fluorescent probe and preparation method thereof, method for selectively detecting iron ions and PPi and cell imaging method
  • Yellow light emission fluorescent probe and preparation method thereof, method for selectively detecting iron ions and PPi and cell imaging method

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preparation example Construction

[0044] In the above preparation method, the purification method can be selected in a wide range, but in order to further increase the yield of nitrogen-doped carbon quantum dots, preferably, after the hydrothermal reaction is completed, the purification includes: centrifuging the system and collecting the upper layer solution, and then dialyze the upper layer solution in a 900-1200Da dialysis bag for 10-14h.

[0045] The present invention also provides a yellow light emitting fluorescent probe prepared by the above preparation method.

[0046] The present invention also provides a selective detection of Fe 3+ methods, including:

[0047] 1) Hydroxyethylpiperazine ethanesulfonic acid HEPES buffer solution, different known concentrations of Fe 3+ The solution and the above-mentioned yellow light-emitting fluorescent probe solution are mixed and constant volume is obtained to obtain a known solution to be tested;

[0048] 2) the Fe in step 1) 3+ After the solution is removed,...

Embodiment 1

[0076] Dissolve 0.2160g o-phenylenediamine in 30mL double-distilled water, and ultrasonically dissolve to obtain a uniform mixed solution. The OPD concentration in the mixed solution is 0.066mmol / L; transfer the mixed solution to a 50ml stainless steel polytetrafluoroethylene high-temperature reactor , hydrothermally reacted at 160°C for 8 hours, took out the reactor and cooled it down to 25°C naturally, after that, collected the upper solution of the product by centrifugation, and dialyzed it with a 1000Da dialysis bag for 12 hours to obtain a nitrogen-doped carbon quantum dot solution, which was stored in the dark for future use .

[0077] TEM characterization of nitrogen-doped carbon quantum dots figure 1 As shown, it can be seen from the figure that the size distribution of nitrogen-doped carbon quantum dots is relatively uniform, and they are close to spherical particles with an average size of 2.6nm, which is consistent with the size distribution characteristics of carbo...

Embodiment 2

[0079] Carry out according to the method of Example 1, the only difference is that the hydrothermal reaction is carried out at 150° C. for 9 hours.

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Abstract

The invention discloses a yellow light emission fluorescent probe and a preparation method thereof, a method for selectively detecting Fe < 3 + > and PPi and a cell imaging method. The preparation method comprises the following steps: carrying out hydrothermal reaction on o-phenylenediamine OPD and water, and then purifying to obtain yellow light nitrogen-doped carbon quantum dots, namely the yellow light emission fluorescent probe. The yellow light emission fluorescent probe has good selectivity and high sensitivity in detection of Fe < 3 + > and PPi, the response time is short, real-time detection can be realized, and the preparation method has the characteristics of simple process and simple operation; in addition, the cell imaging method can realize adjustment of intracellular fluorescence intensity.

Description

technical field [0001] The invention relates to a yellow light emitting fluorescent probe, in particular to a yellow light emitting fluorescent probe and a preparation method thereof, a method for selectively detecting iron ions and PPi, and a cell imaging method. Background technique [0002] Developing anion sensing systems has become an important research area. Therefore, the detection and identification of PPi based on fluorescence changes has become the main focus of many research groups; a precise phosphate anion sensing system could provide a new explanation for the phosphorylation process. [0003] In recent decades, PPi-specific fluorescent probes have been developed, and fluorescence assays have attracted much attention due to their relatively low cost, high sensitivity, easy operation, reliable methods, and low detection limits; although various types of Fluorescent chemosensors can selectively recognize PPi, but only a few fluorescent chemosensors can recognize ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
CPCC09K11/65C09K11/0883B82Y20/00B82Y40/00G01N21/6428G01N21/6486G01N2021/6432
Inventor 杜金艳杨影漆双庆范婷婷王超峰舒琴
Owner ANHUI NORMAL UNIV
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