Core-shell type nano-contrast agent, preparation method and application thereof

A nano-contrast agent and reaction technology, which can be used in pharmaceutical formulations, preparations for in vivo tests, and emulsion delivery, etc., can solve problems such as reduced efficiency and sensitivity, and achieve simple preparation methods, low biological toxicity, and improved biocompatibility. effect of sex and tumor targeting

Inactive Publication Date: 2013-04-17
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, nanoparticles with a diameter larger than 20 nm are easily phagocytosed by the reticuloendothelial system (RES), resulting in reduced efficiency and sensitivity.

Method used

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  • Core-shell type nano-contrast agent, preparation method and application thereof
  • Core-shell type nano-contrast agent, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] a. Preparation of manganese nanoclusters:

[0035] Add 2mL of 2mol / L manganese acetate solution into 30mL of HSA solution (50mg / mL), mix well, then add 1.5mL of 1mol / L NaOH solution, adjust the pH value to be strongly alkaline (pH10.0-14.0), and the obtained The mixed solution was placed on a shaking table at room temperature for 1 h, and the supernatant was collected by centrifugation, which was the manganese nanocluster solution (core-shell nano contrast agent solution).

[0036] b. Purification of core-shell nano-contrast agents:

[0037] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for 24 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Dialyze for 12 hours to remove small inorganic molecules in the solution.

[0038] c. Core-shell nano-contrast agent drying

[0039] The dialysate wa...

Embodiment 2

[0042] a. Preparation of manganese nanoclusters:

[0043] Add 2mL of 1mol / L manganese acetate solution into 30mL of HSA solution (40mg / mL), mix well, then add 0.5mL of 2mol / L NaOH solution, adjust the pH value to be strongly alkaline (pH10.0-14.0), and the obtained The mixed solution was placed on a shaking table at room temperature for 1 h, and the supernatant was collected by centrifugation, which was the manganese nanocluster solution (core-shell nano contrast agent solution).

[0044] b. Purification of core-shell nano-contrast agents:

[0045] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for 72 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Dialyze for 6 hours to remove small inorganic molecules in the solution.

[0046]c. Core-shell nano-contrast agent drying

[0047] The dialysate was ...

Embodiment 3

[0049] a. Preparation of manganese nanoclusters:

[0050] Add 20mL of 2mol / L manganese acetate solution into 300mL of HSA solution (300mg / mL), mix well, then add 2mL of 0.5mol / L NaOH solution, adjust the pH value to be strongly alkaline (pH10.0-14.0), and the obtained The mixture was reacted on a shaker at room temperature for 2 hours, and the supernatant was collected by centrifugation, which was the manganese nanocluster solution (core-shell nano contrast agent solution).

[0051] b. Purification of core-shell nano-contrast agents:

[0052] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for 72 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Internal dialysis for 6 hours to remove small inorganic molecules in the solution.

[0053] c. Core-shell nano-contrast agent drying

[0054] The dialysate...

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Abstract

The invention discloses a core-shell type nano-contrast agent. The core-shell type nano-contrast agent comprises manganese ions and human serum albumin assembled outside the manganese ions and serving as a protecting group. The invention further provides a preparation method of the core-shell type nano-contrast agent. The core-shell type nano-contrast agent disclosed by the invention is combined with a nano-technology, and the human serum albumin is taken as a carrier for wrapping the paramagnetic metal manganese ions, thus MRI (magnetic resonance imaging) is realized, and biocompatibility and a tumor targeting property are improved; the prepared core-shell type nano-contrast agent has the advantages of small particle size and low biotoxicity; and the preparation method provided by the invention is simple.

Description

technical field [0001] The invention relates to molecular imaging technology, in particular to a core-shell nano contrast agent, its preparation method and application. Background technique [0002] Tumor is one of the important diseases that threaten human health. Early diagnosis and treatment of tumor is the key to improve the quality of life and cure rate of patients. At present, molecular imaging technologies applied to tumors include optical imaging technology, CT imaging technology, MRI molecular imaging technology, ultrasonic molecular imaging technology, SPECT technology, PET technology and near-infrared fluorescence imaging technology. Among them, MRI is a medical imaging technology that uses different magnetic resonance signals generated by different tissues of the body under the influence of an external magnetic field for imaging. Imaging, scanning at any orientation level without changing the body position, high contrast without bone artifacts, reflecting the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12
Inventor 蔡林涛胡德红盛宗海龚萍高笃阳张鹏飞
Owner SHENZHEN INST OF ADVANCED TECH
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