Fluorescent SiNDs (silicon nanodots) as well as preparation method and application thereof

A silicon nanometer and fluorescent technology, applied in the fields of nanomaterials and biology, can solve the problems of short imaging time, high cost, poor stability, etc., and achieve the effects of simple preparation method, good cleaning resistance, and good biocompatibility

Active Publication Date: 2017-11-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to provide a fluorescent silicon nano-dot (SiNDs) to realize ultra-long-time, cleaning-resistant

Method used

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  • Fluorescent SiNDs (silicon nanodots) as well as preparation method and application thereof
  • Fluorescent SiNDs (silicon nanodots) as well as preparation method and application thereof
  • Fluorescent SiNDs (silicon nanodots) as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0047] Example 1

[0048] Taking AEEA as an example, the preparation of fluorescent silicon nanodots includes the following steps:

[0049] (1) Weigh and fully dissolve Rose Bengal in ultrapure water, add an appropriate volume of AEEA, so that the mass ratio of AEEA and Rose Bengal is 100:3. Mix the two thoroughly and transfer them to the hydrothermal reactor;

[0050] (2) Reaction: react in a hydrothermal reactor at 160°C for 3 hours to form a silicon nanodot solution;

[0051] (3) Purification: Dialysis to obtain the target super bright fluorescent silicon nanodot solution.

[0052] See the schematic diagram of the reaction figure 1 , The transmission electron microscope results of the prepared SiNDs can be seen figure 2 , See the statistical results of particle size distribution image 3 , And its ultraviolet-visible absorption spectrum see Figure 4 , The fluorescence excitation emission spectrum can be seen Figure 5 , Its extinction at 650nm in different pH environments can be s...

Example Embodiment

[0053] Example 2

[0054] To test the lysosomal imaging effect of the SiNDs prepared in Example 1 on A549 cells, the method is as follows:

[0055] (1) Cell culture: divide A549 cells into 5×10 3 The density of each well will be planted in a 96-well plate at 37℃, 5% CO 2 Culture for 24h in the environment;

[0056] (2) Cell staining: replace the culture medium in each well with a fresh culture medium containing 20 g / mL SiNDs, at 37℃, 5% CO 2 After incubating for 2, 4, 18 and 24 hours in the environment, they were washed twice with phosphate buffered saline (PBS). Then, it was stained with a commercial red lysosomal fluorescent dye (LT-Red) and a nuclear staining reagent (Hoechst 33342) for 30 and 10 minutes, respectively. Finally, wash the cells twice with PBS;

[0057] (3) Confocal fluorescence microscopy imaging observation: Using lasers with wavelengths of 405, 488 and 552nm as excitation light, Hoechst 33342 dye emits blue fluorescence under the excitation of 405nm excitation lig...

Example

[0059] Examples 3 and 4

[0060] Test the lysosomal imaging effect of commercial lysosomal green fluorescent dye (LT-Green) and red fluorescent dye (LT-Red) on A549 cells.

[0061] The operation process of Examples 3 and 4 is basically the same as that of Example 1, except that the SiNDs in step (2) are replaced with 1LT-Green and LT-Red and incubated for 30 minutes, 4 hours and 8 hours respectively.

[0062] See the effect of lysosomal imaging Figure 8 with 9 . Observed Figure 8 with Picture 9 It can be seen that the change of dosing time has little effect on the green lysosomal fluorescent dye LT-Green, but it greatly changes the staining site of LT-Red. As the dosing time was extended to 4h, LT-Red was transferred to the cytoplasm and nuclear membrane other than lysosomes. Even at 8h, some dyes had entered the nucleus. Therefore, compared with LT-Red, the synthesized SiNDs of the present invention are more suitable for long-term tracking lysosomal imaging.

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Abstract

The invention discloses fluorescent SiNDs (silicon nanodots). The fluorescent SiNDs are prepared from silane and Bengal rose with a hydrothermal method through one step. Compared with the prior art, the prepared SiNDs have ultrahigh fluorescent quantum yield (100%) and can realize long-time specific imaging of mammal cytolysosome. Besides, the imaging effect of lysosome of the SiNDs is not affected by cell cleaning, immobilization, permeabilization and the like and the fluorescent SiNDs have the advantages of cleaning resistance, immobilization resistance and permeabilization resistance. Meanwhile, the SiNDs also have the advantages of low preparation cost, simple synthesis method, good water dispersity, narrow fluorescent emission peak width, good photostability, good cytocompatibility, low photocytotoxicity and the like and are expected to become novel lysosome fluorescent probes.

Description

technical field [0001] The invention belongs to the field of nano-materials and biotechnology, and in particular relates to a fluorescent silicon nano-dot and its preparation method and application. Background technique [0002] Fluorescent materials are widely used in biomedicine. Compared with traditional organic fluorescent small molecules, fluorescent nanomaterials have attracted extensive attention in recent years because of their advantages such as better photostability and tunable excitation and emission wavelengths. At present, there are many types of fluorescent nanomaterials, but they all have certain shortcomings. For example, traditional semiconductor quantum dots (such as cadmium selenide, lead sulfide, etc.) generally contain toxic heavy metal elements, so they are widely used in biomedicine. The use dose is greatly limited; although the biocompatibility of noble metal nanoclusters (such as gold nanoclusters, silver nanoclusters) and upconversion nanoparticles...

Claims

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Application Information

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IPC IPC(8): C09K11/59B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/59G01N21/6458
Inventor 吴富根陈晓凯张晓东
Owner SOUTHEAST UNIV
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