Fluorescent test strip for quantitatively detecting thyroglobulin and preparation method of fluorescent test strip

A technology of thyroglobulin and test strips, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems that FNA-Tg technology has not attracted enough attention

Pending Publication Date: 2020-07-07
WUXI PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been recognized abroad and widely used clinically, but...

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  • Fluorescent test strip for quantitatively detecting thyroglobulin and preparation method of fluorescent test strip
  • Fluorescent test strip for quantitatively detecting thyroglobulin and preparation method of fluorescent test strip
  • Fluorescent test strip for quantitatively detecting thyroglobulin and preparation method of fluorescent test strip

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Embodiment 1

[0035] A preparation method for a fluorescent test strip for detecting thyroglobulin, comprising the steps of:

[0036] (1) Pretreatment of the sample pad: soak the glass fiber membrane in the buffer solution of the sample pad to completely soak it, take it out, drain it and dry it for later use;

[0037] (2) Pretreatment of the bonding pad: Soak another piece of glass fiber membrane in the buffer of the bonding pad until it is completely soaked, take it out, drain it and dry it for later use;

[0038](3) Preparation of fluorescent microsphere-labeled thyroglobulin antibody: Nano-quantum dot fluorescent microspheres with a particle size of 100nm were added to reaction buffer and 10mg / ml EDC, mixed at room temperature for 0.5h, centrifuged at 10,000rpm for 10min, and precipitated with 0.05M Dissolve in / LMES buffer or 0.02M / LPBS, resuspend by ultrasonication; add 1 μl of anti-thyroglobulin antibody to the above resuspension, oscillate to mix, sonicate for 10s, mix and react at ...

Embodiment 2

[0043] A preparation method for a fluorescent test strip for detecting thyroglobulin, comprising the steps of:

[0044] (1) Pretreatment of the sample pad: soak the glass fiber membrane in the buffer solution of the sample pad to completely soak it, take it out, drain it and dry it for later use;

[0045] (2) Pretreatment of the bonding pad: Soak another piece of glass fiber membrane in the buffer of the bonding pad until it is completely soaked, take it out, drain it and dry it for later use;

[0046] (3) Preparation of fluorescent microsphere-labeled thyroglobulin antibody: Nano-quantum dot fluorescent microspheres with a particle size of 150nm were added to reaction buffer and 10mg / ml EDC, mixed at room temperature for 0.8h, centrifuged at 12000rpm for 20min, and precipitated with 0.05M Dissolve in / LMES buffer or 0.02M / LPBS, resuspend by ultrasonication; add 10 μl of anti-thyroglobulin antibody to the above resuspension, oscillate and mix, ultrasonicate for 11s, mix at roo...

Embodiment 3

[0051] A preparation method for a fluorescent test strip for detecting thyroglobulin, comprising the steps of:

[0052] (1) Pretreatment of the sample pad: soak the glass fiber membrane in the buffer solution of the sample pad to completely soak it, take it out, drain it and dry it for later use;

[0053] (2) Pretreatment of the bonding pad: Soak another piece of glass fiber membrane in the buffer of the bonding pad until it is completely soaked, take it out, drain it and dry it for later use;

[0054] (3) Preparation of fluorescent microsphere-labeled thyroglobulin antibody: Nano-quantum dot fluorescent microspheres with a particle size of 200nm were added to reaction buffer and 10mg / ml EDC, mixed at room temperature for 1h, centrifuged at 15000rpm for 30min, and precipitated with 0.05M / ml Dissolve in LMES buffer or 0.02M / LPBS, resuspend by ultrasonication; add 20 μl of anti-thyroglobulin antibody to the above resuspension, oscillate to mix, sonicate for 12 s, mix at room tem...

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Abstract

The invention relates to a fluorescent test strip for quantitatively detecting thyroglobulin and a preparation method of the fluorescent test strip. The test strip sequentially comprises a PVC plate,a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad from bottom to top, wherein the combination pad is coated with an anti-thyroglobulin antibody labeled by nano quantum dot fluorescent microspheres, the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is coated with another anti-thyroglobulin antibody, and the quality control line is coated with goat anti-mouse IgG. The preparation method is simple, steps easy to operate, the prepared fluorescent test strip can be used for rapidly detecting the Tg valueof thyroglobulin of suspicious lymph nodes on site; the test strip can also be used for lymph node fine needle puncture, elution and Tg value measurement of preoperative, intraoperative and postoperative follow-up visits, and assists in clear diagnosis of suspicious neck lymph node thyroid papillary cancer metastasis. The test strip provided by the invention is simple and convenient in use method,high in detection speed and strong in specificity.

Description

technical field [0001] The invention relates to a fluorescent test strip for detecting thyroglobulin and a preparation method thereof, belonging to the technical field of biological reagents. Background technique [0002] The surgical methods, follow-up treatment, and follow-up of patients with thyroid cancer with cervical lymph node metastasis are completely different. If routine preventive dissection of cervical lymph nodes is performed, the disadvantages outweigh the advantages. In the postoperative follow-up of thyroid cancer, the recurrence rate of lymph node metastasis is 3%-30%, and the diagnosis rate is 27%-46%. Therefore, early identification of cervical lymph node metastasis is very important. Although US-FNA is very specific for the diagnosis of cervical metastatic lymph nodes, its sensitivity is low, especially for samples with low cellularity. Therefore, early detection of cervical lymph node metastasis is one of the key points and difficulties in the individu...

Claims

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Application Information

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IPC IPC(8): G01N33/58G01N33/68
CPCG01N33/588G01N33/582G01N33/68
Inventor 周锋盛丁炎张雨刘元端木家喜吴鹏西
Owner WUXI PEOPLES HOSPITAL
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