A kind of detection method of carbendazim

A detection method and technology for carbendazim, applied in the detection field of carbendazim, can solve the problems of high detection cost, small detection range and long processing time, and achieve the effects of high detection sensitivity, fast detection speed and low detection limit

Active Publication Date: 2020-11-24
CHONGQING UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a detection method for carbendazim, which solves the problems of long sample pretreatment time, complicated operation, low sensitivity, small detection range and high detection cost in the existing detection method

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  • A kind of detection method of carbendazim
  • A kind of detection method of carbendazim
  • A kind of detection method of carbendazim

Examples

Experimental program
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Effect test

Embodiment 1

[0037] 1) Add 0.5g m-phenylenediamine and 2.84g diethylenetriamine pentamethylidene phosphonic acid to 30ml of water to obtain a reaction solution, then ultrasonicate for 5min to make the reaction solution evenly mixed, and then pour the reaction solution into a 50ml reaction kettle After reacting at 200 °C for 5 h, take it out, naturally cool to room temperature, centrifuge at 10,000 rpm for 10 min to obtain the supernatant, then pour the supernatant into a 1000 MWCO dialysis bag and dialyze for 24 h to obtain the carbon dot stock solution. pH=6) diluted 800,000 times to obtain a carbon dot detection solution for detection and set aside.

[0038] 2) Add 2g of chloroauric acid tetrahydrate into 200ml of water, heat it to boiling, then add 6ml of freshly prepared 1% trisodium citrate aqueous solution, stir well, and when the color of the mixed solution changes from black to red, continue to heat for 30 minutes. Take out, cool to room temperature naturally to obtain nano-gold so...

Embodiment 2

[0042] 1) Add 0.5g m-phenylenediamine and 2.84g diethylenetriamine pentamethylidene phosphonic acid to 30ml of water to obtain a reaction solution, then ultrasonicate for 5min to make the reaction solution evenly mixed, then pour the reaction solution into a 50ml reactor at 200°C After 5 hours of reaction, take it out, cool to room temperature naturally, centrifuge at 10,000 rpm for 10 minutes to obtain the supernatant, then pour the supernatant into a 1000 MWCO dialysis bag and dialyze for 24 hours to obtain the carbon dot stock solution. The carbon dot stock solution is buffered with different pH values ​​B-R respectively. The solution was diluted 800,000 times to obtain a carbon dot detection solution for detection, and the pH values ​​were 4, 4.5, 5, 5.5, 6, 6.5, 7, 8 and 9, respectively.

[0043] 2) Add 2g of chloroauric acid tetrahydrate into 200ml of water, heat it to boiling, then add 6ml of freshly prepared 1% trisodium citrate aqueous solution, stir well, and when the...

Embodiment 3

[0047] The test method is the same as in Example 2, wherein the pH value of the B-R buffer solution is 6, and the incubation time is variable, which are 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 min respectively. Taking the fluorescence recovery intensity as the ordinate and the incubation time as the abscissa, draw a curve graph, and the results are as follows Figure 4 shown.

[0048] from Figure 4 It can be seen that under the same conditions of other factors, with the increase of incubation time, the fluorescence recovery intensity of carbon dots is also continuously enhanced. When the incubation time was 5 min, the carbendazim in the mixed solution fully reacted with the gold nanoparticles, and the fluorescence recovery intensity would not increase if the incubation time was increased.

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Abstract

The invention discloses a detection method of carbendazim. According to the detection method, the concentration of the carbendazim in an analyte solution is detected on the basis of green light-emitting fluorescent carbon dots and noble metal nanogold, wherein the nanogold can quench fluorescence of the carbon dots through fluorescence resonance energy transfer, and the carbendazim can be bonded to the nanogold so as to restore the quenched fluorescence of the carbon dots. During detection of the carbendazim, operation is simple, the detection specificity is good, the detection sensitivity ishigh, the limit of detection is low, the lower limit is 0.45ppb, the detection speed is high, the detection efficiency is greatly improved, the stability is good, the cost is low and real-time onlinefast and specific detection can be achieved; the detection method can be applied to simple and fast detection of carbendazim pesticide residues in fruits and vegetables. The detection method has a good application prospect and a potential application value in the field of detection analysis.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a detection method for carbendazim. Background technique [0002] Carbendazim, a benzimidazole drug, is also the main metabolite of benomyl in mammals and its degradation product in the environment. Carbendazim, as a broad-spectrum and high-efficiency fungicide, is widely used in the control of powdery mildew and downy mildew of various agricultural products such as vegetables and fruits. , resulting in chronic or acute toxicity and affect human health, can cause excitement, convulsions, trance, nausea and vomiting, dizziness, headache, chest tightness, epigastric tenderness and other poisoning symptoms through the esophagus. [0003] At present, the detection methods of carbendazim mostly rely on gas, liquid chromatography and its combination with mass spectrometry, fluorescence spectroscopy, electrochemical sensors, and chemical analysis methods. Although the ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 侯长军杨奕霞霍丹群侯经洲刘自山
Owner CHONGQING UNIV
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