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Dressing for promoting wound healing and preparation method of dressing

A wound healing and mixed solution technology, applied in the field of biomedical engineering, can solve the problems of poor barrier effect, poor wound healing effect, exogenous infection, etc., and achieve the effect of repairing wounds, maintaining factor activity, and inhibiting protease activity

Active Publication Date: 2020-07-10
江苏奥普莱医疗用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional dressings also have obvious disadvantages. For example, the dressing has poor barrier function after being penetrated by wound fluid, which is easy to cause exogenous infection and has poor wound healing effect.

Method used

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  • Dressing for promoting wound healing and preparation method of dressing

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0021] alpha 2 The preparation method of M crude extract:

[0022] Add 0.1% (w / v) soybean trypsin inhibitor to fresh porcine plasma at -5°C, and add EDTA (ethylenediaminetetraacetic acid) solution to make the final concentration to 10mmol / L. 18% Polyethylene Glycol 6000 fractional precipitation, after fully stirring for 60min, centrifuge at 10000r / min for 30min at 4°C, dissolve the precipitate in 50mmol / L Tris-HCl (pH7. .4) The buffer solution is fully dialyzed to obtain α 2 M crude extract.

Embodiment 1

[0024] 1. Take α 2 After the M crude extract is concentrated, through Sephacryl S-300 gel chromatography column, eluent is the 100mmol / L Tris-HCl (pH8.0) damping fluid that contains 0.5mol / LNaCl, detects effluent under absorption peak 280nm, The first peak effluent was collected and concentrated using an ultrafiltration membrane with a molecular weight cut-off of 400kDa to obtain α 2 Macroglobulin solution, whose protein concentration was determined to be 90 μg / L by BCA protein quantification kit;

[0025] 2. Mix 0.42g blue copper peptide, 0.12g copper sulfate, 2.2g leupeptin, and 5g viscosity-average molecular weight to 5.0×10 5 , carboxymethyl chitosan with a degree of deacetylation of 82.5% and 15g of average molecular weight of 1.0×10 6 Dissolve polyethylene oxide in 1L of water, stir evenly to obtain a mixed solution, then add 1.25mL of α 2 Macroglobulin solution to obtain spinning solution;

[0026] 3. Electrospinning is carried out with spinning solution, the used s...

Embodiment 2

[0028] 1. Take α 2 After the M crude extract is concentrated, through Sephacryl S-300 gel chromatography column, eluent is the 100mmol / L Tris-HCl (pH8.0) damping fluid that contains 0.5mol / LNaCl, detects effluent under absorption peak 280nm, The first peak effluent was collected and concentrated using an ultrafiltration membrane with a molecular weight cut-off of 300kDa to obtain α 2 Macroglobulin solution, whose protein concentration was determined to be 80 μg / L by BCA protein quantification kit;

[0029] 2. Mix 0.17g blue copper peptide, 0.08g copper sulfate, 1.1g leupeptin, and 5g viscosity-average molecular weight to 5.0×10 5 , the degree of deacetylation is 80% carboxymethyl chitosan and 5g average molecular weight is 1.0×10 6 Dissolve polyethylene oxide in 1L of water, stir evenly to obtain a mixed solution, then add 1mL of the α prepared in step 1 2 Macroglobulin solution to obtain spinning solution;

[0030] 3. Electrospinning is carried out with spinning solution,...

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Abstract

The invention relates to the field of biomedical engineering, and discloses a dressing for promoting wound healing and a preparation method of the dressing. The method includes the following steps: (1) purifying alpha2 macroglobulin; (2) dissolving GHK-Cu, copper sulfate, leupeptin, carboxymethyl chitosan and polyethylene oxide in water, performing uniform stirring, and adding the alpha2 macroglobulin solution to obtain a spinning solution; and (3) performing electrospinning by using the spinning solution to obtain the dressing for promoting wound healing. The dressing for promoting wound healing prepared by the method is a medical material that can induce wound healing for a long time, can accelerate wound healing, and is very effective in treating wounds and skin injury.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a dressing for promoting wound healing and a preparation method thereof. Background technique [0002] After skin injury, medical dressings are usually required to protect the wound, prevent wound infection and severe dehydration, provide an environment conducive to wound healing, and promote wound healing. At present, traditional dressings such as medical absorbent cotton gauze, cotton pads and vaseline gauze are the most widely used dressings for skin wounds in clinical practice. The traditional dressing has a net-like weaving structure, which is cheap, relatively simple in production process, wide in source of raw materials, soft in texture, has strong absorption capacity, can prevent wound exudate from accumulating, and has a certain degree of protective effect on wound healing. Widely used in skin wounds. However, traditional dressings also have obvious disadvantages....

Claims

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Application Information

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IPC IPC(8): A61L15/18A61L15/26A61L15/28A61L15/32A61L15/42A61L15/44
CPCA61L15/18A61L15/26A61L15/28A61L15/32A61L15/42A61L15/44A61L2300/102A61L2300/252A61L2300/412C08L89/00C08L5/08C08L71/02
Inventor 汤佳鹏吴小梅葛彦朱俐万峥
Owner 江苏奥普莱医疗用品有限公司
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