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Stem cell cryopreservation liquid and cryopreservation method

A cryopreservation method and stem cell technology, which is applied in the field of stem cell cryopreservation solution and cryopreservation, can solve problems such as differences between serum batches, virus infection, immune rejection, etc., and achieve consistent morphology, high safety, and survival rate high effect

Inactive Publication Date: 2020-07-17
山东万能干细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, animal-derived serum has the risk of infection with pathogens such as viruses and bacteria, and some of the proteins in it may cause immune rejection
In addition, serum has problems such as batch-to-batch variation and instability

Method used

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  • Stem cell cryopreservation liquid and cryopreservation method
  • Stem cell cryopreservation liquid and cryopreservation method
  • Stem cell cryopreservation liquid and cryopreservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The main component of the stem cell cryopreservation medium is DMEM-F12 basal medium, and the added components are human serum albumin, stem cell growth factor, γ-polyglutamic acid and glycerol;

[0026] The concentration of the added components in the freezing solution is as follows:

[0027]

[0028]

Embodiment 2

[0030] The main component of the stem cell cryopreservation medium is DMEM-F12 basal medium, and the added components are human serum albumin, stem cell growth factor, γ-polyglutamic acid and glycerol;

[0031] The concentration of the added components in the freezing solution is as follows:

[0032]

Embodiment 3

[0034] The main component of the stem cell cryopreservation solution is physiological saline, and the added components are human serum albumin, stem cell growth factor, γ-polyglutamic acid and glycerol;

[0035] The concentration of the added components in the freezing solution is as follows:

[0036]

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Abstract

The invention belongs to the field of cells and bioengineering, and particularly relates to a stem cell cryopreservation liquid and a cryopreservation method. The stem cell cryopreservation liquid iscomposed of a main component and an additive component, the main component is PBS, normal saline or a basal culture medium, and the additive component is human serum albumin, stem cell growth factors,gamma-polyglutamic acid and glycerin. A stem cell suspension is centrifuged, supernatant is removed, the product is mixed with the stem cell cryopreservation liquid, and subpackaging and cryopreserving are carried. According to the invention, DMSO and FBS are not contained; gamma-polyglutamic acid glycerol is matched differently; the cryopreservation liquid can obtain better freezing effect by combining with human serum albumin, has strong water molecule complexing capacity, can reduce the formation of ice crystals in cells in the freezing process, can be used for cryopreservation of cells, and has the positive effects of consistent forms before and after cell cryopreservation, high survival rate after cell resuscitation and good proliferation capacity of cells after cryopreservation resuscitation.

Description

technical field [0001] The invention belongs to the field of cell and bioengineering, and in particular relates to a stem cell cryopreservation solution and a cryopreservation method. Background technique [0002] Stem cells have been widely used in scientific research and clinical treatment due to their self-renewal and multi-directional differentiation potential. Such as embryonic stem cells, induced pluripotent stem cells and mesenchymal stem cells. Stem cells have a variety of uses and functions, and can be used to study the growth and development of tissues and organs in vitro, establish disease models, drug screening, and clinical treatment. Mesenchymal stem cells in stem cells have been widely used in therapeutic research of animal disease models and human clinical trials. Diseases that mesenchymal stem cells can treat include diabetes, liver cirrhosis, skin damage, myocardial infarction, and nerve damage. In addition, mesenchymal stem cells also have the functions...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 李昱儒李研
Owner 山东万能干细胞生物技术有限公司
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