Hippophae rhamnoides seed oil extract and application thereof
A technology of seabuckthorn seed oil and extract, which is applied in the field of biomedicine, can solve problems such as excessive pigmentation, skin allergies, and affect appearance, and achieve good curative effect, high scientific research value, and good application prospects
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Embodiment 1
[0018] Example 1 Preparation method of sea buckthorn seed oil extract B1.
[0019] A preparation method of sea buckthorn seed oil extract, the specific steps are as follows.
[0020] The crude oil of sea buckthorn seeds and petroleum ether were mixed evenly in a volume ratio (1:2), and absolute ethanol (0.5 V) was added for extraction, and the liquid was separated. The upper layer was the petroleum ether phase, and the lower layer was the absolute ethanol phase. The water-ethanol phase is Fr.1. Take Fr.1 for saponification, shake and centrifuge at 5000 r / min for 15 min. The white colloids in the middle layer were combined, and 20% sulfuric acid was added according to the body to shake. After standing, petroleum ether (2 V) was added to shake, and centrifuged at 5000 r / min for 15 min. Take the upper petroleum ether phase, add distilled water (1 V), shake repeatedly, adjust the pH to neutral, centrifuge at 5000 r / min for 15 min, take the petroleum ether phase, add distilled wa...
Embodiment 2
[0021] Example 2 Pharmacological study on anti-atopic dermatitis of a topical preparation for the treatment of AD.
[0022] An external preparation for treating AD, comprising the following raw materials by weight: 5 parts of sea buckthorn seed oil extract B1, 7 parts of olive oil, 5 parts of glyceryl monostearate, 3 parts of silicone oil, 4 parts of Tween 80, 1.5 parts of lanolin, 5 parts of glycerin, 3 parts of cetyl alcohol, 66.5 parts of water.
[0023] A specific preparation method of an external preparation for treating AD: all raw materials are placed in a beaker and heated to 75° C. with stirring, then kept in a 75° C. water bath for heat preservation, and stirred at 1000 r / min for 40 minutes with a stirrer. After cooling, store at 4°C for later use.
[0024] A research experiment on the pharmacological action of a topical preparation for AD treatment.
[0025] In order to evaluate the therapeutic effect of sea buckthorn oil on AD inflammation, the inventors prepared...
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