Application of porphyra haitanensis polysaccharide in invigorating stomach and preventing or repairing gastrointestinal injury

A technology of laver and polysaccharides, which is applied in the field of polysaccharides of laver, to achieve the effects of enhancing adhesion, improving nutritional value, and repairing gastrointestinal damage

Active Publication Date: 2020-07-24
SHANTOU MEIHENG TETRA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Previous studies have shown that squid ink polysaccharides have a protective effect on intestinal tight junctions and adherens junctions under chemotherapy conditions, but the application of Porphyra laver polysaccharides (PHP) in the repair and protection of gastrointestinal tract has not been reported

Method used

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  • Application of porphyra haitanensis polysaccharide in invigorating stomach and preventing or repairing gastrointestinal injury
  • Application of porphyra haitanensis polysaccharide in invigorating stomach and preventing or repairing gastrointestinal injury
  • Application of porphyra haitanensis polysaccharide in invigorating stomach and preventing or repairing gastrointestinal injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Preparation of Porphyra haitanensis polysaccharide

[0026] Porphyra haitanensis polysaccharides are prepared by the following method: take dried Porphyra chinensis as a raw material, pulverize, add distilled water 4 times the mass of the raw material for extraction, (temperature 90℃, stirring for 2h), filter the filtrate, and add 3 times the volume to the filtrate 95% ethanol, placed in a refrigerator at 4°C for 12-24 hours, and filtered; the precipitate was dissolved in distilled water and lyophilized to obtain the crude Porphyra polysaccharide; the crude Porphyra polysaccharide was purified by DEAE ion exchange resin and chlorinated at 0.2M The sodium solution is eluted, the component liquid is collected, dialyzed, and lyophilized to obtain purified Porphyra haitanensis polysaccharide PHP. The sugar content of the Porphyra haitanensis polysaccharide prepared by the above method is 72.4%, protein content is 0.58%, and uronic acid content is 9.37%.

Embodiment 2

[0027] Example 2: The effect of Porphyra haitanensis polysaccharide on cell proliferation

[0028] The polysaccharide solution of Porphyra haitanensis was prepared at 25μg / mL, 50μg / mL, 100μg / mL, 200μg / mL, 400μg / mL in DMEM medium, and cultured with IEC-6 cells for 24h and 48h; The cells of the laver polysaccharide solution culture were counted and analyzed, and the results were as follows figure 1 As shown, the polysaccharide of Porphyra haitanensis has a certain proliferation effect on IEC-6, and the effect of proliferation is related to the concentration of polysaccharide and the time of culture. Under 24h of culture, 100μg / mL Porphyra haitanensis polysaccharide PHP has performance on IEC-6. Significant proliferation (*p <0.05).

Embodiment 3

[0029] Example 3: Repairing effect of Porphyra haitanensis polysaccharide PHP on scratches

[0030] At 6.25×10 4 Inoculate IEC-6 cells into a six-well culture plate at a cell density of one cell per square centimeter, and culture for 24 hours. After the cells reach 90% confluency, use a 200 μL pipette tip to scratch the cells in five places and use PBS The sloughed cells were washed away with buffer solution. The experimental group was cultured with a medium containing 100μg / mL Porphyra haitan polysaccharide PHP, and the control group was cultured with medium only. The three time points were 0, 12 and 24 hours. The healing of the scratches was examined and recorded under a microscope, and the area of ​​the scratches was statistically analyzed. The result is figure 2 As shown, after 12h and 24h incubation of the scratches, the scratch area of ​​the control group and the experimental group showed a decreasing trend, while the culture medium containing 100μg / mL Porphyra haitanensis...

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Abstract

The invention discloses application of porphyra haitanensis polysaccharide in invigorating stomach and preventing or repairing gastrointestinal injury. The porphyra haitanensis polysaccharide disclosed by the invention can play a role in proliferation and repair of injured IEC-6 cells, can effectively enhance the adhesion of IEC-6 cells, and up-regulate the expression of 8 proteins such as Cdc-42,Rac-1, Par-3, aPKC, PKC beta II, paxillin, FAK and GAPDH in IEC-6 cells. When being used as a raw material of food such as rice porridge, the porphyra haitanensis polysaccharide not only can improvethe nutritional value of the rice porridge and enrich the taste and flavor of the rice porridge, but also has the effects of invigorating stomach and preventing and repairing gastrointestinal injury.

Description

Technical field [0001] The invention relates to the application field of Porphyra haitanensis polysaccharides, in particular to the application of Porphyra haitanensis polysaccharides in strengthening the stomach, preventing or repairing gastrointestinal injuries. Background technique [0002] As one of the important economic seaweeds for large-scale cultivation in my country, Porphyra yezoensis belongs to the genus Rhodophyta, Laveraceae, and Porphyra. It is abundant in coastal areas such as Guangdong, Fujian and Zhejiang. Porphyra haitanensis polysaccharide is one of the active ingredients in Porphyra haitanensis. It is a multi-component water-soluble polysaccharide, which belongs to galactose polysulfate, 1,3-β-D-galactose and 1,4-α-L- The 3,6-internal ether galactose consists of disaccharide repeating units alternately linked, sometimes substituted by β-D-galactose-6-methoxy and α-L-galactose-6-sulfate groups. Studies have shown that Porphyra haitanensis polysaccharide has m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/125A23L7/10A23L19/10A23L25/00A23L31/00C08B37/00A21D2/18
CPCA23L33/125A23L7/198A23L19/10A23L25/30A23L31/00C08B37/006C08B37/0003A23V2002/00A21D2/181A23V2200/32A23V2250/51A23V2300/10A23V2300/20
Inventor 张杰良邱华迈苏雷什
Owner SHANTOU MEIHENG TETRA BIOTECH CO LTD
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