Method for preparing human placenta extract supernate

Abstract

The invention provides a method for preparing human placenta extract supernate. The method comprises the following steps: removing excess blood vessels and tissue elements from a full-term eutocous placenta, and preparing placenta tissue homogenate by virtue of mechanical method; cleaning placenta tissue homogenate, and carrying out deep-low-temperature repeated freezing and thawing; carrying outcentrifugation on the repeatedly frozen and thawed placenta tissue homogenate, carrying out filtration, and extracting supernate; and inspecting the supernate, detecting the content of growth factors,and meanwhile, detecting germs, fungi, mycoplasmas and endotoxin, so as to obtain a final supernate product. According to the method, four growth factors are released and highly expressed in the prepared placenta tissue homogenate, and a supernate product with high content of growth factors can be acquired by regulating the volume of the final supernate product; and pollution risks of pathogenicmicroorganisms, the germs, the fungi, the mycoplasmas and the endotoxin are avoided in the preparation process of the supernate, so that the safety and effectiveness are guaranteed and improved.

Description

technical field [0001] The invention belongs to the field of extraction, preparation and application of placenta; in particular, it relates to a preparation method of human placenta extract supernatant. Background technique [0002] The placenta is a special tissue for mammalian viviparous animals to provide nutrients for the fetus during pregnancy and allow the embryo to grow. It is excreted during childbirth. Because of its special barrier function and endocrine function, it has been valued by people since ancient times. Modern biological and medical research has proved that the placenta contains immunoglobulins that can enhance the body's immunity and inhibit virus from invading cells; it contains 17 kinds of amino acids, including 7 kinds of essential amino acids, and most of the amino acids in the extract exist in the form of polypeptides. It is easy to be absorbed and utilized by the body; it contains a variety of hormones including human chorionic gonadotropin, prolac...

AI Technical Summary

Problems solved by technology

[0003] At present, in the research of placenta extracts, most of the studies on placenta extracts are mainly based on the enzymes, polypeptides, amino acids, and collagen in the placenta, and various chemical additives are added to achieve the application effect, but due to its The extraction method is complicated, and many chemical and biological reagents are used in the extraction and preparation, resulting in a large change in the active ingredients, and the extract contains more impurities and complex ingredients, so it is not suitable for skin care products.

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