Large-view-field microscope objective lens

A technology of microscope objective lens and large field of view, which is applied in the field of large field of view microscope objective lens, which can solve the problems of large objective lens volume and achieve the effects of reduced volume, shortened length and high numerical aperture of the objective lens

Pending Publication Date: 2020-07-31
苏州溢博伦光电仪器有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The object of the present invention is to provide a kind of objective lens of large field of view microscope, to solve the problem that the objective lens of existing large field of view microscope is bulky

Method used

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  • Large-view-field microscope objective lens
  • Large-view-field microscope objective lens
  • Large-view-field microscope objective lens

Examples

Experimental program
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Embodiment approach 1

[0021] as attached figure 1 Shown: the large field of view microscope objective lens of the present embodiment comprises lens 1, lens 2, lens 3, lens 4, lens 5 and scanner 6, and scanner 6 is a single uniaxial scanner or a single biaxial scanner, The scanner 6 is used to focus the reflected excitation beam in the sample, collect the emitted optical signal excited by the sample, and enter the optical fiber for transmitting the optical signal or the photodetector for detecting the optical signal after being coupled by the focusing lens. The back focal plane of the objective lens calculated according to the wavelength of the excitation light is located outside the lens body of the objective lens, and the scanner 6 is located on the back focal plane calculated according to the wavelength of the excitation light of this embodiment. The lens 1 has a surface S11 on the opposite image side and a surface S12 on the opposite object side, the lens 2 has a surface S21 on the opposite imag...

Embodiment approach 2

[0034] The difference between this embodiment and Embodiment 1 is that in this embodiment, the scanner adopts a set of two single-axis scanners, and the rear focal plane of the objective lens is located in the middle of the set of two single-axis scanners.

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Abstract

The invention belongs to the technical field of optical microscopic imaging, and particularly discloses a large-view-field microscope objective lens. The objective lens comprises a scanner and a plurality of lenses. A back focal plane calculated by the objective lens according to excitation light wavelength is positioned outside an objective lens body. When the scanner is a single single-axis scanner or a single double-axis scanner, the single single-axis scanner or the single double-axis scanner is positioned on the rear focal plane of the objective lens. When the scanners are two single-axisscanners in one group, the rear focal plane of the objective lens is positioned in the middle of the two single-axis scanners in one group. According to the invention, the design concepts that the diameter of an incident light beam is kept unchanged, the field angle is increased and the rear focal plane of the objective lens is externally arranged are adopted, and the high-numerical-aperture large-field imaging microscope objective lens with low cost and small size is provided for a standard desk type multi-photon microscope.

Description

technical field [0001] The invention relates to the technical field of optical microscopic imaging, in particular to a large field of view microscope objective lens. Background technique [0002] Traditional microscope objective lens is an optical system with large aperture angle and small field angle. Since the microscope objective lens is used to provide extremely high resolution (about 200nm, regardless of super-resolution technology), its high magnification results in a small field of view. In 2001, Martin Oheim et al. ("Two-photon microscopy in brain tissue:parameters influencing the imaging depth", Journal of Neuroscience Methods.111: 29-37.) conducted a review on the performance of high-magnification objective lenses and low-magnification objective lenses with approximate numerical apertures. In contrast, it is pointed out that increasing the front aperture of the objective lens is of great benefit to improving the collection efficiency of fluorescence, especially fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/02G02B21/00
CPCG02B21/002G02B21/02
Inventor 不公告发明人
Owner 苏州溢博伦光电仪器有限公司
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