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Composite microbial agent with biocontrol and nutrition effects

A technology of compound microbial agent and nutrition, which is applied in the direction of biocides, chemicals for biological control, biocides, etc., can solve problems such as difficult dissolution, low sub-utilization rate, excessive drug residues in agricultural products, etc., and achieve improvement The effect of loosening the soil, increasing the utilization rate, and inhibiting the occurrence of pests

Inactive Publication Date: 2020-08-07
山东土木启生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the low utilization rate of calcium ions in plants and the difficulty in dissolving; the improper use of pesticides has caused excessive drug residues in agricultural products and serious environmental pollution. Screening and isolation of some beneficial bacteria with high control effect on plant diseases and insect pests, to obtain dominant strains, through complementary advantages to ferment a variety of beneficial bacteria according to a certain process, combined with some plant medium elements and biosynergists, It has a good control effect on plant diseases and pests while supplementing nutrition, and also has the effect of improving soil. It has the advantages of simple preparation method, obvious effect, and improved economic benefits, so as to solve the defects in the prior art.

Method used

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  • Composite microbial agent with biocontrol and nutrition effects
  • Composite microbial agent with biocontrol and nutrition effects
  • Composite microbial agent with biocontrol and nutrition effects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Weigh 60g of molasses, 50g of brown sugar, 10g of peptone, 55g of bean cake powder, 0.8g of potassium dihydrogen phosphate, 0.4g of magnesium sulfate, and 4g of yeast powder. After mixing the above materials evenly, add them to a 5L triangle flask, add water After dissolving, add water until the solvent volume is 1L, sterilize at 121°C for 30 minutes, cool down to 37°C, inoculate plantaractobacillus plantarum into a 5L Erlenmeyer flask at a temperature of 37°C and a speed of 120rpm , inoculate Lactobacillus casei according to the inoculation amount of 140g / L in the Erlenmeyer flask of 5L after cultivating 12h;

[0041] Step 2: After inoculating the Lactobacillus casei in the culture medium, cultivate it for 8 hours at a temperature of 37°C and a stirring speed of 120rpm, and start to add a calcium carbonate suspension with a concentration of 20%, and raise the stirring speed to 180rpm, and the carbonic acid The concentration of calcium carbonate in the mixture o...

Embodiment 2

[0047] Step 1: Weigh 60g of molasses, 50g of brown sugar, 10g of peptone, 55g of bean cake powder, 0.8g of potassium dihydrogen phosphate, 0.4g of magnesium sulfate, and 4g of yeast powder. After mixing the above materials evenly, add them to a 5L triangle flask, add water After dissolving, add water until the volume of the solvent is 1L, sterilize at 121°C for 30 minutes, cool down to 37°C, add Lactobacillus plantarum into a 5L Erlenmeyer flask at a rate of 180g / L, at a temperature of 36°C and a speed of 120rpm After cultivating for 12 hours, add Lactobacillus casei in the 5L Erlenmeyer flask according to the addition of 170g / L;

[0048] Step 2: After inoculating Lactobacillus casei in the culture medium, at a temperature of 37°C and a stirring speed of 120rpm, cultivate it for 8h, start to add a calcium carbonate suspension with a concentration of 22%, and raise the stirring speed to 170rpm, when When the concentration of calcium carbonate in the mixture of calcium carbonate...

Embodiment 3

[0056] Step 1: Weigh 60g of molasses, 50g of brown sugar, 10g of peptone, 55g of bean cake powder, 0.8g of potassium dihydrogen phosphate, 0.4g of magnesium sulfate, and 4g of yeast powder. After mixing the above materials evenly, add them to a 5L triangular flask and add water to dissolve Then add water until the volume of the solvent is 1L, sterilize at 121°C for 30min, cool down to 37°C, add 140g of plant Lactobacillus to a 5L triangular flask, cultivate it for 12h at a temperature of 36°C and a speed of 120rpm, and then transfer it to a 5L triangular flask Add 180g Lactobacillus casei to the bottle;

[0057] Step 2: After inoculating the Lactobacillus casei in the culture medium, at a temperature of 36°C and a stirring speed of 120rpm, cultivate for 8h, start to add a calcium carbonate suspension with a concentration of 23%, and raise the stirring speed to 160rpm, when the carbonic acid When the concentration of calcium carbonate in the mixture of the calcium suspension an...

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Abstract

The invention relates to a compound microbial agent with biocontrol and nutrition effects. The compound microbial agent comprises the following substances with the concentration: 1-10 billion / mL of bacillus subtilis, 1-10 billion / mL of bacillus thuringiensis, 0.01-0.1 billion / mL of lactobacilluus paracase, 0.01-0.1 billion / mL of lactobacillus plantarum, 200-300 g / L of calcium lactate, 7-10 g / L ofchitosan, 30-50 g / L of fulvic acid, and the balance being water. The compound microbial agent is a soluble high-calcium nutrient fertilizer and also contains four biocontrol nutrient bacteria, so thatplant diseases and insect pests can be effectively prevented and treated as soon as possible in the early stage of disease attack, and the effect of supplementing nutrient elements and the biocontrolbacteria at the same time is achieved.

Description

technical field [0001] The invention belongs to the field of agricultural bio-control nutrient fertilizer, in particular to a compound microbial bacterial agent with bio-control nutrition function. Background technique [0002] Our country has a vast land area and feeds nearly 20% of the population with 9% of the world's arable land. In order to continuously increase the output of food, vegetables and fruits, a large amount of chemical fertilizers and pesticides are invested every year. Ecological problems are becoming more and more prominent. We urgently need to find a method that can A new planting model that reduces environmental pressure requires us to reduce the input of chemical fertilizers, increase the utilization rate of fertilizers, reduce the use of pesticides, and gradually restore the soil environment to a good state. [0003] Nitrogen, phosphorus, potassium, calcium, magnesium, sulfur, boron, iron, zinc and other large, medium and trace elements in chemical fer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05G3/80C05G5/20A01N63/22A01N63/23A01N63/20A01N61/00A01N43/16A01P1/00A01P3/00A01P7/00A01P21/00
CPCA01N43/16A01N61/00A01N63/20A01N63/22A01N63/23C05F11/00C05G3/60C05G3/80C05G5/20C05F11/08C05F11/10
Inventor 郑安科张俊庆孙亚敏赵宇茜尹迎春
Owner 山东土木启生物科技有限公司
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