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Four-color laser illumination fluorescence microscope

A fluorescence microscope and laser illumination technology, applied in the field of microscopy, can solve the problems of insufficient three-dimensional imaging capabilities of SPIM microscopes, and achieve the effects of optimizing real-time imaging performance, improving spatial resolution and optical tomography capabilities, and high integration.

Pending Publication Date: 2020-08-07
锘海生物科学仪器(上海)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem solved by this application is the defect of insufficient three-dimensional imaging capability of the existing SPIM microscope

Method used

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  • Four-color laser illumination fluorescence microscope

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Embodiment Construction

[0022] In order to make the above purpose, features and beneficial effects of the present application more obvious and understandable, specific embodiments of the present application will be described in detail below in conjunction with the accompanying drawings. Apparently, the described embodiments are some of the embodiments of the present application, but not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

[0023] In the description of this application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description...

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Abstract

The invention discloses a four-color laser illumination fluorescence microscope and relates to the technical field of microscopes, the four-color laser illumination fluorescence microscope comprises afour-color laser light source system, a dichroscope group, a first reflector, a beam expanding collimation system, a first half-wave plate, a spatial light modulation system, a 4f system, a scanninggalvanometer system, a triangular prism system, a left light emitting system, a right light emitting system, a left air objective lens, a right air objective lens, a microscope, a filter wheel, a camera and a 3D translation stage. The microscope is provided with the four-color laser light source system and the dichroscope set, four lasers are integrated, the microscope is suitable for imaging of different tissue samples, and an operator can select the proper laser wavelength for imaging according to the characteristics of the samples. The four-color laser is suitable for most samples. The contradiction among the spatial resolution, the optical tomography capability and the field of view is overcome, the spatial resolution and the optical tomography capability are improved, the real-time imaging performance is optimized, and the integration level is higher.

Description

technical field [0001] The present application relates to the technical field of microscopy, in particular to a four-color laser-illuminated fluorescence microscope. Background technique [0002] Due to the inherent three-dimensional characteristics of biological tissues, the research and exploration of life sciences (such as the study of brain nerve projections, blood vessel distribution, and tumor microenvironment) requires in-depth research based on three-dimensional spatial information of biological tissues. The traditional research on thinner section samples or a small number of cells can no longer meet the needs of researchers, and the corresponding traditional imaging methods (such as three-dimensional imaging of samples by confocal microscopy) are no longer applicable. [0003] In recent years, processing methods for large-scale biological samples (whole brain and spinal cord of mice, etc.) and three-dimensional microscopic imaging methods have gradually emerged. Se...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/17G01J3/44G01J3/10
CPCG01N21/6458G01N21/64G01N21/17G01J3/4406G01J3/10G01N2021/6417G01N2021/6467G01N2021/1785G01J2003/102
Inventor 殷明
Owner 锘海生物科学仪器(上海)有限公司