Tracing method of probiotics in dairy product

A technology of probiotics and dairy products, applied in the field of detection, can solve the problems of not including probiotics, poor DNA extraction effect, unsatisfactory test results, etc., and achieve the effect of improving the effect
CN107058482AInactive Publication Date: 2017-08-18PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2017-08-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of detection, relates to a tracing method of probiotics in a dairy product, and particularly relates to a tracing method of probiotics in the dairy product on an Illumina Miseq sequencing platform. The method comprises the following steps: (1) sample pretreatment: adding sodium citrate and sodium hydroxide into a to-be-detected sample, mixing and centrifuging, discarding supernatant, and adding lysozyme into precipitates for incubation; (2) genomic DNA extraction of the pretreated sample; (3) 16S rRNA PCR amplification: aiming at v1-v3 regions and v5-v6 regions of bacterium 16s rDNA, and designing a primer for PCR amplification; and (4) Illumina Miseq sequencing: performing Illumina Miseq sequencing to the PCR product. Analysis on bacterial strain types and abundance of probiotics contained in food of market fermented dairy products, probiotic milk powder and the like is conducted by utilizing the Illumina Miseq sequencing platform, showing that the practical abundance of probiotics in the solid probiotic products is basically identical with the labeling, and the practical sequencing in the fermented type probiotic product has great difference from that of the labeled bacterial strain.
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Description

technical field

[0001] The invention relates to the technical field of detection, and relates to a traceability method for probiotics in dairy products, in particular to a traceability method for probiotics in dairy products by an Illumina Miseq sequencing platform. Background technique

[0002] The composition of intestinal flora is closely related to human nutrition, immunity, cancer and aging. It promotes the proliferation of beneficial bacteria in the intestine and inhibits the growth of harmful bacteria, which is of great significance for preventing diseases caused by poor living habits. The application of probiotics has also become more and more extensive with the deepening of research. At present, the microorganisms used as probiotics are all derived from humans or animals. Probiotic products mostly use Lactobacillus, Streptococcus, and Bifidobacterium in lactic acid bacteria. strains. With the progress of probiotic research and the popularization of knowledge, the p...

Claims

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