Method for detecting novel cardiovascular disease risk marker by liquid chromatography-tandem mass spectrometry

A high-performance liquid chromatography and mass spectrometer technology, applied in the field of liquid chromatography tandem mass spectrometry detection of new cardiovascular disease risk markers, can solve the problems of complex processing methods, inability to automate, and high cost, and achieves simple processing methods and convenient clinical promotion. , easy to automate effects

Pending Publication Date: 2020-08-07
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE +1
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Problems solved by technology

[0009] 1. Most methods can only detect TMAO, which cannot meet clinical needs;
[0010] 2. The pretreatment method of biological samples is too complicated to be automated, and it is difficult to promote it to clinical application;
[0011] 3. The clinical significance of the detection markers is not clear, and there is not enough literature and data support
[0012] 4. There are too many irrelevant markers and the cost is too high

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  • Method for detecting novel cardiovascular disease risk marker by liquid chromatography-tandem mass spectrometry
  • Method for detecting novel cardiovascular disease risk marker by liquid chromatography-tandem mass spectrometry
  • Method for detecting novel cardiovascular disease risk marker by liquid chromatography-tandem mass spectrometry

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Embodiment Construction

[0047] 1. In order to better illustrate the technical features and effects of the present invention, the following uses specific embodiments to describe the present invention in detail, but the present invention is not limited thereto.

[0048] (1) Calibration of standard solution

[0049] (a) Preparation of standard working solution:

[0050] Accurately weigh 10.00mg of trimethylamine oxide, choline, betaine, creatinine and L-carnitine standard substances respectively in a 10ml volumetric flask with an analytical balance, and dilute to the mark with 50% methanol to obtain a standard stock solution with a concentration of 1.00mg / mL, use 50% methanol solution to dilute from the standard stock solution to obtain the standard intermediate solution with a concentration of 100 μg / mL;

[0051] Take 20μL, 200μL, 200μL, 200μL and 200μL of the above standard intermediate solution respectively, then add 180μL of 50% methanol solution, mix well, dilute with aqueous solution, in the sol...

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Abstract

The invention belongs to the technical field of clinical biological sample detection, and particularly relates to a method for detecting trimethylamine oxide, choline, betaine, creatinine and L-carnitine in a biological sample through high-throughput liquid chromatography-tandem mass spectrometry. The method can simultaneously detect five substances in a biological sample, and has the advantages of high accuracy, high sensitivity, high specificity, small required sample volume, simple sample pretreatment, good tolerance and the like.

Description

technical field [0001] The invention relates to a method for detecting trimethylamine oxide, choline, betaine, L-carnitine and creatinine in biological samples by high-throughput liquid chromatography tandem mass spectrometry. Background technique [0002] The prevalence and mortality of cardiovascular disease (CVD) in China continue to rise, and the number of patients is about 290 million. CVD mortality ranks first among all causes, accounting for more than 40% of residents' disease deaths. At the same time, the burden of cardiovascular disease in China is increasing day by day and has become a major public health problem. With the development of social economy, the national lifestyle has undergone profound changes. Under the background of social aging and accelerated urbanization, and the prevalence of unhealthy lifestyles among residents, national CVD risk factors are generally exposed, and the prevalence of hypertension, dyslipidemia, and diabetes continues to increase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/32G01N30/34G01N30/72G01N2030/045G01N2030/324
Inventor 周洲王恺隽武红梅赵丽平高小晶栗琳
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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